cDNA Clone HDAC3

• Plasmid: pCMV-TAG3b HDAC3
• Parent Plasmid: pCMV tag3b
• Expressed: Myc-HDAC3
• Genbank: NM_003883
• Tag: DAC
• Species: Human

Sequence of Insert

ggatccATGGCCAAGACCGTGGCCTATTTCTACGACCCCGACGTGGGCAA
CTTCCACTACGGAGCTGGACACCCTATGAAGCCCCATCGCCTGGCATTGA
CCCATAGCCTGGTCCTGCATTACGGTCTCTATAAGAAGATGATCGTCTTC
AAGCCATACCAGGCCTCCCAGCATGACATGTGCCGCTTCCACTCCGAGGA
CTACATTGACTTCCTGCAGAGAGTCAGCCCCACCAATATGCAAGGCTTCA
CCAAGAGTCTTAATGCCTTCAACGTAGGCGATGACTGCCCAGTGTTTCCC
GGGCTCTTTGAGTTCTGCTCGCGTTACACAGGCGCATCTCTGCAAGGAGC
AACCCAGCTGAACAACAAGATCTGTGATATTGCCATTAACTGGGCTGGTG
GTCTGCACCATGCCAAGAAGTTTGAGGCCTCTGGCTTCTGCTATGTCAAC
GACATTGTGATTGGCATCCTGGAGCTGCTCAAGTACCACCCTCGGGTGCT
CTACATTGACATTGACATCCACCATGGTGACGGGGTTCAAGAAGCTTTCT
ACCTCACTGACCGGGTCATGACGGTGTCCTTCCACAAATACGGAAATTAC
TTCTTCCCTGGCACAGGTGACATGTATGAAGTCGGGGCAGAGAGTGGCCG
CTACTACTGTCTGAACGTGCCCCTGCGGGATGGCATTGATGACCAGAGTT
ACAAGCACCTTTTCCAGCCGGTTATCAACCAGGTAGTGGACTTCTACCAA
CCCACGTGCATTGTGCTCCAGTGTGGAGCTGACTCTCTGGGCTGTGATCG
ATTGGGCTGCTTTAACCTCAGCATCCGAGGGCATGGGGAATGCGTTGAAT
ATGTCAAGAGCTTCAATATCCCTCTACTCGTGCTGGGTGGTGGTGGTTAT
ACTGTCCGAAATGTTGCCCGCTGCTGGACATATGAGACATCGCTGCTGGT
AGAAGAGGCCATTAGTGAGGAGCTTCCCTATAGTGAATACTTCGAGTACT
TTGCCCCAGACTTCACACTTCATCCAGATGTCAGCACCCGCATCGAGAAT
CAGAACTCACGCCAGTATCTGGACCAGATCCGCCAGACAATCTTTGAAAA
CCTGAAGATGCTGAACCATGCACCTAGTGTCCAGATTCATGACGTGCCTG
CAGACCTCCTGACCTATGACAGGACTGATGAGGCTGATGCAGAGGAGAGG
GGTCCTGAGGAGAACTATAGCAGGCCAGAGGCACCCAATGAGTTCTATGA
TGGAGACCATGACAATGACAAGGAAAGCGATGTGGAGATTTAAggatcc

Amino Acid Sequence

MEQKLISEEDLSPGGSMAKTVAYFYDPDVGNFHYGAGHPMKPHRLALTHS
LVLHYGLYKKMIVFKPYQASQHDMCRFHSEDYIDFLQRVSPTNMQGFTKS
LNAFNVGDDCPVFPGLFEFCSRYTGASLQGATQLNNKICDIAINWAGGLH
HAKKFEASGFCYVNDIVIGILELLKYHPRVLYIDIDIHHGDGVQEAFYLT
DRVMTVSFHKYGNYFFPGTGDMYEVGAESGRYYCLNVPLRDGIDDQSYKH
LFQPVINQVVDFYQPTCIVLQCGADSLGCDRLGCFNLSIRGHGECVEYVK
SFNIPLLVLGGGGYTVRNVARCWTYETSLLVEEAISEELPYSEYFEYFAP
DFTLHPDVSTRIENQNSRQYLDQIRQTIFENLKMLNHAPSVQIHDVPADL
LTYDRTDEADAEERGPEENYSRPEAPNEFYDGDHDNDKESDVEI

• Vector Type: Mammalian
• Antibiotic: Kan
• DU Number: DU7142
• Unit Source: Protein Phosphorylation and Ubiquitylation Unit
• Location: PPU
• MTA: Dundee
• Notes: Construct may rearrange in DH5∝ cells, but higher stability is obtained in the E.coli strain SCS110 (Stratagene).; ; Estimated MW of expressed protein : 50548 Daltons