cDNA Clone HDAC3

• Plasmid: pEGFP-C1-HDAC3
• Parent Plasmid: pEGFPC1
• Expressed: GFP-HDAC3
• Genbank: NM_003883
• Tag: GFP
• Species: Human

Sequence of Insert

ggatccATGGCCAAGACCGTGGCCTATTTCTACGACCCCGACGTGGGCAA
CTTCCACTACGGAGCTGGACACCCTATGAAGCCCCATCGCCTGGCATTGA
CCCATAGCCTGGTCCTGCATTACGGTCTCTATAAGAAGATGATCGTCTTC
AAGCCATACCAGGCCTCCCAGCATGACATGTGCCGCTTCCACTCCGAGGA
CTACATTGACTTCCTGCAGAGAGTCAGCCCCACCAATATGCAAGGCTTCA
CCAAGAGTCTTAATGCCTTCAACGTAGGCGATGACTGCCCAGTGTTTCCC
GGGCTCTTTGAGTTCTGCTCGCGTTACACAGGCGCATCTCTGCAAGGAGC
AACCCAGCTGAACAACAAGATCTGTGATATTGCCATTAACTGGGCTGGTG
GTCTGCACCATGCCAAGAAGTTTGAGGCCTCTGGCTTCTGCTATGTCAAC
GACATTGTGATTGGCATCCTGGAGCTGCTCAAGTACCACCCTCGGGTGCT
CTACATTGACATTGACATCCACCATGGTGACGGGGTTCAAGAAGCTTTCT
ACCTCACTGACCGGGTCATGACGGTGTCCTTCCACAAATACGGAAATTAC
TTCTTCCCTGGCACAGGTGACATGTATGAAGTCGGGGCAGAGAGTGGCCG
CTACTACTGTCTGAACGTGCCCCTGCGGGATGGCATTGATGACCAGAGTT
ACAAGCACCTTTTCCAGCCGGTTATCAACCAGGTAGTGGACTTCTACCAA
CCCACGTGCATTGTGCTCCAGTGTGGAGCTGACTCTCTGGGCTGTGATCG
ATTGGGCTGCTTTAACCTCAGCATCCGAGGGCATGGGGAATGCGTTGAAT
ATGTCAAGAGCTTCAATATCCCTCTACTCGTGCTGGGTGGTGGTGGTTAT
ACTGTCCGAAATGTTGCCCGCTGCTGGACATATGAGACATCGCTGCTGGT
AGAAGAGGCCATTAGTGAGGAGCTTCCCTATAGTGAATACTTCGAGTACT
TTGCCCCAGACTTCACACTTCATCCAGATGTCAGCACCCGCATCGAGAAT
CAGAACTCACGCCAGTATCTGGACCAGATCCGCCAGACAATCTTTGAAAA
CCTGAAGATGCTGAACCATGCACCTAGTGTCCAGATTCATGACGTGCCTG
CAGACCTCCTGACCTATGACAGGACTGATGAGGCTGATGCAGAGGAGAGG
GGTCCTGAGGAGAACTATAGCAGGCCAGAGGCACCCAATGAGTTCTATGA
TGGAGACCATGACAATGACAAGGAAAGCGATGTGGAGATTTAAggatcc

Amino Acid Sequence

MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTLKFICT
TGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIF
FKDDGNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHN
VYIMADKQKNGIKVNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNH
YLSTQSALSKDPNEKRDHMVLLEFVTAAGITLGMDELYKSGLRSRAQASN
SAVDGTAGPGSMAKTVAYFYDPDVGNFHYGAGHPMKPHRLALTHSLVLHY
GLYKKMIVFKPYQASQHDMCRFHSEDYIDFLQRVSPTNMQGFTKSLNAFN
VGDDCPVFPGLFEFCSRYTGASLQGATQLNNKICDIAINWAGGLHHAKKF
EASGFCYVNDIVIGILELLKYHPRVLYIDIDIHHGDGVQEAFYLTDRVMT
VSFHKYGNYFFPGTGDMYEVGAESGRYYCLNVPLRDGIDDQSYKHLFQPV
INQVVDFYQPTCIVLQCGADSLGCDRLGCFNLSIRGHGECVEYVKSFNIP
LLVLGGGGYTVRNVARCWTYETSLLVEEAISEELPYSEYFEYFAPDFTLH
PDVSTRIENQNSRQYLDQIRQTIFENLKMLNHAPSVQIHDVPADLLTYDR
TDEADAEERGPEENYSRPEAPNEFYDGDHDNDKESDVEI

• Vector Type: Mammalian
• Antibiotic: Kan
• DU Number: DU7141
• Unit Source: Protein Phosphorylation and Ubiquitylation Unit
• Location: PPU
• MTA: Dundee
• Notes: Construct may rearrange in DH5∝ cells, but higher stability is obtained in the E.coli strain SCS110 (Stratagene).