cDNA Clone GBL

• Plasmid: pGEX6P-GBL-beta
• Parent Plasmid: pGEX6P1
• Expressed: GST-GBL
• Genbank: NM_022372
• Tag: GST
• Species: Human

Sequence of Insert

ggatccATGAACACCTCCCCAGGCACGGTGGGCAGTGACCCGGTCATCCT
GGCCACTGCAGGCTACGACCACACCGTGCGCTTCTGGCAGGCCCACAGCG
GCATCTGCACCCGGACGGTGCAGCACCAGGACTCCCAGGTGAATGCCTTG
GAGGTCACACCGGACCGCAGCATGATTGCTGCTGCAGGTTACCAGCACAT
CCGCATGTATGATCTCAACTCCAATAACCCTAACCCCATCATCAGCTACG
ACGGCGTCAACAAGAACATCGCGTCTGTGGGCTTCCACGAAGACGGCCGC
TGGATGTACACGGGCGGCGAGGACTGCACAGCCAGGATCTGGGACCTCAG
GTCCCGGAACCTGCAGTGCCAGCGGATCTTCCAGGTGAACGCACCCATTA
ACTGCGTGTGCCTGCACCCGAACCAGGCAGAGCTCATCGTGGGTGACCAG
AGCGGGGCTATCCACATCTGGGACTTGAAAACAGACCACAACGAGCAGCT
GATCCCTGAGCCCGAGGTCTCCATCACGTCCGCCCACATCGATCCCGACG
CCAGCTACATGGCAGCTGTCAATAGCACCGGAAACTGCTATGTCTGGAAT
CTGACGGGGGGCATTGGTGACGAGGTGACCCAGCTCATCCCCAAGACTAA
GATCCCTGCCCACACGCGCTACGCCCTGCAGTGTCGCTTCAGCCCCGACT
CCACGCTCCTCGCCACCTGCTCGGCTGATCAGACGTGCAAGATCTGGAGG
ACGTCCAACTTCTCCCTGATGACGGAGCTGAGCATCAAGAGCGGCAACCC
CGGGGAGTCCTCCCGCGGCTGGATGTGGGGCTGCGCCTTCTCGGGGGACT
CCCAGTACATCGTCACTGGTGAGCCCCGCCCTGGCCTCCCCCATCCCTGG
CCCCCGGCGCTGGCCTCCAGAGCCAGCCCACCTCGGCTGCAGCTTCCCCT
CTGCTGGGGCCGCCTGCTTGGCCTGCACCTGCGCTCTTAGgcggccgc

Amino Acid Sequence

MSPILGYWKIKGLVQPTRLLLEYLEEKYEEHLYERDEGDKWRNKKFELGL
EFPNLPYYIDGDVKLTQSMAIIRYIADKHNMLGGCPKERAEISMLEGAVL
DIRYGVSRIAYSKDFETLKVDFLSKLPEMLKMFEDRLCHKTYLNGDHVTH
PDFMLYDALDVVLYMDPMCLDAFPKLVCFKKRIEAIPQIDKYLKSSKYIA
WPLQGWQATFGGGDHPPKSDLEVLFQGPLGSMNTSPGTVGSDPVILATAG
YDHTVRFWQAHSGICTRTVQHQDSQVNALEVTPDRSMIAAAGYQHIRMYD
LNSNNPNPIISYDGVNKNIASVGFHEDGRWMYTGGEDCTARIWDLRSRNL
QCQRIFQVNAPINCVCLHPNQAELIVGDQSGAIHIWDLKTDHNEQLIPEP
EVSITSAHIDPDASYMAAVNSTGNCYVWNLTGGIGDEVTQLIPKTKIPAH
TRYALQCRFSPDSTLLATCSADQTCKIWRTSNFSLMTELSIKSGNPGESS
RGWMWGCAFSGDSQYIVTGEPRPGLPHPWPPALASRASPPRLQLPLCWGR
LLGLHLRS

• Vector Type: Bacterial
• Antibiotic: Amp
• DU Number: DU7053
• Unit Source: Protein Phosphorylation and Ubiquitylation Unit
• Location: PPU
• MTA: Dundee
• Notes: Construct for bacterial expression of GST N-terminal fusion (with PreScission Protease cleavage site).; ; Estimated MW of expressed protein : 62861 Daltons