cDNA Clone ROCK2

• Synonyms: ROCKII
• Plasmid: pCMV5-FLAG-1 ROCK2
• Parent Plasmid: pCMV5 FLAG1
• Expressed: FLAG-ROCK2
• Genbank: NP_004841.2
• Tag: FLAG
• Species: Human

Sequence of Insert

GGATCCATGAGCCGGCCCCCGCCGACGGGGAAAATGCCCGGCGCCCCCGA
GACCGCGCCGGGGGACGGGGCAGGCGCGAGCCGCCAGAGGAAGCTGGAGG
CGCTGATCCGAGACCCTCGCTCCCCCATCAACGTGGAGAGCTTGCTGGAT
GGCTTAAATTCCTTGGTCCTTGATTTAGATTTTCCTGCTTTGAGGAAAAA
CAAGAACATAGATAATTTCTTAAATAGATATGAGAAAATTGTGAAAAAAA
TCAGAGGTCTACAGATGAAGGCAGAAGACTATGATGTTGTAAAAGTTATT
GGAAGAGGTGCTTTTGGTGAAGTGCAGTTGGTTCGTCACAAGGCATCGCA
GAAGGTTTATGCTATGAAGCTTCTTAGTAAGTTTGAAATGATAAAAAGAT
CAGATTCTGCCTTTTTTTGGGAAGAAAGAGATATTATGGCCTTTGCCAAT
AGCCCCTGGGTGGTTCAGCTTTTTTATGCCTTTCAAGATGATAGGTATCT
GTACATGGTAATGGAGTACATGCCTGGTGGAGACCTTGTAAACCTTATGA
GTAATTATGATGTGCCTGAAAAATGGGCCAAATTTTACACTGCTGAAGTT
GTTCTTGCTCTGGATGCAATACACTCCATGGGTTTAATACACAGAGATGT
GAAGCCTGACAACATGCTCTTGGATAAACATGGACATCTAAAATTAGCAG
ATTTTGGCACGTGTATGAAGATGGATGAAACAGGCATGGTACATTGTGAT
ACAGCAGTTGGAACACCGGATTATATATCACCTGAGGTTCTGAAATCACA
AGGGGGTGATGGTTTCTATGGGCGAGAATGTGATTGGTGGTCTGTAGGTG
TTTTCCTTTATGAGATGCTAGTGGGGGATACTCCATTTTATGCGGATTCA
CTTGTAGGAACATATAGCAAAATTATGGATCATAAGAATTCACTGTGTTT
CCCTGAAGATGCAGAAATTTCCAAACATGCAAAGAATCTCATCTGTGCTT
TCTTAACAGATAGGGAGGTACGACTTGGGAGAAATGGGGTGGAAGAAATC
AGACAGCATCCTTTCTTTAAGAATGATCAGTGGCATTGGGATAACATAAG
AGAAACGGCAGCTCCTGTAGTACCTGAACTCAGCAGTGACATAGACAGCA
GCAATTTCGATGACATTGAAGATGACAAAGGAGATGTAGAAACCTTCCCA
ATTCCTAAAGCTTTTGTTGGAAATCAGCTGCCTTTCATCGGATTTACCTA
CTATAGAGAAAATTTATTATTAAGTGACTCTCCATCTTGTAGAGAAACTG
ATTCCATACAATCAAGGAAAAATGAAGAAAGTCAAGAGATTCAGAAAAAA
CTGTATACATTAGAAGAACATCTTAGCAATGAGATGCAAGCCAAAGAGGA
ACTGGAACAGAAGTGCAAATCTGTTAATACTCGCCTAGAAAAAACAGCAA
AGGAGCTAGAAGAGGAGATTACCTTACGGAAAAGTGTGGAATCAGCATTA
AGACAGTTAGAAAGAGAAAAGGCGCTTCTTCAGCACAAAAATGCAGAATA
TCAGAGGAAAGCTGATCATGAAGCAGACAAAAAACGAAATTTGGAAAATG
ATGTTAACAGCTTAAAAGATCAACTTGAAGATTTGAAAAAAAGAAATCAA
AACTCTCAAATATCCACTGAGAAAGTGAATCAACTCCAGAGACAACTGGA
TGAAACCAATGCTTTACTGCGAACAGAGTCTGATACTGCAGCCCGGTTAA
GGAAAACCCAGGCAGAAAGTTCAAAACAGATTCAGCAGCTGGAATCTAAC
AATAGAGATCTACAAGATAAAAACTGCCTGCTGGAGACTGCCAAGTTAAA
ACTTGAAAAGGAATTTATCAATCTTCAGTCAGCTCTAGAATCTGAAAGGA
GGGATCGAACCCATGGATCAGAGATAATTAATGATTTACAAGGTAGAATA
TGTGGCCTAGAAGAAGATTTAAAGAACGGCAAAATCTTACTAGCGAAAGT
AGAACTGGAGAAGAGACAACTTCAGGAGAGATTTACTGATTTGGAAAAGG
AAAAAAGCAACATGGAAATAGATATGACATACCAACTAAAAGTTATACAG
CAGAGCCTAGAACAAGAAGAAGCTGAACATAAGGCCACAAAGGCACGACT
AGCAGATAAAAATAAGATCTATGAGTCCATCGAAGAAGCCAAATCAGAAG
CCATGAAAGAAATGGAGAAGAAGCTCTTGGAGGAAAGAACTTTAAAACAG
AAAGTGGAGAACCTATTGCTAGAAGCTGAGAAAAGATGTTCTCTATTAGA
CTGTGACCTCAAACAGTCACAGCAGAAAATAAATGAGCTCCTTAAACAGA
AAGATGTGCTAAATGAGGATGTTAGAAACCTGACATTAAAAATAGAGCAA
GAAACTCAGAAGCGCTGCCTTACACAAAATGACCTGAAGATGCAAACACA
ACAGGTTAACACACTAAAAATGTCAGAAAAGCAGTTAAAGCAAGAAAATA
ACCATCTCATGGAAATGAAAATGAACTTGGAAAAACAAAATGCTGAACTT
CGAAAAGAACGTCAGGATGCAGATGGGCAAATGAAAGAGCTCCAGGATCA
GCTCGAAGCAGAACAGTATTTCTCAACCCTTTATAAAACACAAGTTAGGG
AGCTTAAAGAAGAATGTGAAGAAAAGACCAAACTTGGTAAAGAATTGCAG
CAGAAGAAACAGGAATTACAGGATGAACGGGACTCTTTGGCTGCCCAACT
GGAGATCACCTTGACCAAAGCAGATTCTGAGCAACTGGCTCGTTCAATTG
CTGAAGAACAATATTCTGATTTGGAAAAAGAGAAGATCATGAAAGAGCTG
GAGATCAAAGAGATGATGGCTAGACACAAACAGGAACTTACGGAAAAAGA
TGCTACAATTGCTTCTCTTGAGGAAACTAATAGGACACTAACTAGTGATG
TTGCCAATCTTGCAAATGAGAAAGAAGAATTAAATAACAAATTGAAAGAT
GTTCAAGAGCAACTGTCAAGATTGAAAGATGAAGAAATAAGCGCAGCAGC
TATTAAAGCACAGTTTGAGAAGCAGCTATTAACAGAAAGAACACTCAAAA
CTCAAGCTGTGAATAAGTTGGCTGAGATCATGAATCGAAAAGAACCTGTC
AAGCGTGGTAATGACACAGATGTGCGGAGAAAAGAGAAGGAGAATAGAAA
GCTACATATGGAGCTTAAATCTGAACGTGAGAAATTGACCCAGCAGATGA
TCAAGTATCAGAAAGAACTGAATGAAATGCAGGCACAAATAGCTGAAGAG
AGCCAGATTCGAATTGAACTGCAGATGACATTGGACAGTAAAGACAGTGA
CATTGAGCAGCTGCGGTCACAACTCCAAGCCTTGCATATTGGTCTGGATA
GTTCCAGTATAGGCAGTGGACCAGGGGATGCTGAGGCAGATGATGGGTTT
CCAGAATCAAGATTAGAAGGATGGCTTTCATTGCCTGTACGAAACAACAC
TAAGAAATTTGGATGGGTTAAAAAGTATGTGATTGTAAGCAGTAAGAAGA
TTCTTTTCTATGACAGTGAACAAGATAAAGAACAATCCAATCCTTACATG
GTTTTAGATATAGACAAGTTATTTCATGTCCGACCAGTTACACAGACAGA
TGTGTATAGAGCAGATGCTAAAGAAATTCCAAGGATATTCCAGATTCTGT
ATGCCAATGAAGGAGAAAGTAAGAAGGAACAAGAATTTCCAGTGGAGCCA
GTTGGAGAAAAATCTAATTATATTTGCCACAAGGGACATGAGTTTATTCC
TACTCTTTATCATTTCCCAACCAACTGTGAGGCTTGTATGAAGCCCCTGT
GGCACATGTTTAAGCCTCCTCCTGCTTTGGAGTGCCGCCGTTGCCACATT
AAGTGTCATAAAGATCATATGGACAAAAAGGAGGAGATTATAGCACCTTG
CAAAGTATATTATGATATTTCAACGGCAAAGAATCTGTTATTACTAGCAA
ATTCTACAGAAGAGCAGCAGAAGTGGGTTAGTCGGTTGGTGAAAAAGATA
CCTAAAAAGCCCCCAGCTCCAGACCCTTTTGCCCGATCATCTCCTAGAAC
TTCAATGAAGATACAGCAAAACCAGTCTATTAGACGGCCAAGTCGACAGC
TTGCCCCAAACAAACCTAGCTAAGCGGCCGCG

Amino Acid Sequence

MDYKDDDDKGSMSRPPPTGKMPGAPETAPGDGAGASRQRKLEALIRDPRS
PINVESLLDGLNSLVLDLDFPALRKNKNIDNFLNRYEKIVKKIRGLQMKA
EDYDVVKVIGRGAFGEVQLVRHKASQKVYAMKLLSKFEMIKRSDSAFFWE
ERDIMAFANSPWVVQLFYAFQDDRYLYMVMEYMPGGDLVNLMSNYDVPEK
WAKFYTAEVVLALDAIHSMGLIHRDVKPDNMLLDKHGHLKLADFGTCMKM
DETGMVHCDTAVGTPDYISPEVLKSQGGDGFYGRECDWWSVGVFLYEMLV
GDTPFYADSLVGTYSKIMDHKNSLCFPEDAEISKHAKNLICAFLTDREVR
LGRNGVEEIRQHPFFKNDQWHWDNIRETAAPVVPELSSDIDSSNFDDIED
DKGDVETFPIPKAFVGNQLPFIGFTYYRENLLLSDSPSCRETDSIQSRKN
EESQEIQKKLYTLEEHLSNEMQAKEELEQKCKSVNTRLEKTAKELEEEIT
LRKSVESALRQLEREKALLQHKNAEYQRKADHEADKKRNLENDVNSLKDQ
LEDLKKRNQNSQISTEKVNQLQRQLDETNALLRTESDTAARLRKTQAESS
KQIQQLESNNRDLQDKNCLLETAKLKLEKEFINLQSALESERRDRTHGSE
IINDLQGRICGLEEDLKNGKILLAKVELEKRQLQERFTDLEKEKSNMEID
MTYQLKVIQQSLEQEEAEHKATKARLADKNKIYESIEEAKSEAMKEMEKK
LLEERTLKQKVENLLLEAEKRCSLLDCDLKQSQQKINELLKQKDVLNEDV
RNLTLKIEQETQKRCLTQNDLKMQTQQVNTLKMSEKQLKQENNHLMEMKM
NLEKQNAELRKERQDADGQMKELQDQLEAEQYFSTLYKTQVRELKEECEE
KTKLGKELQQKKQELQDERDSLAAQLEITLTKADSEQLARSIAEEQYSDL
EKEKIMKELEIKEMMARHKQELTEKDATIASLEETNRTLTSDVANLANEK
EELNNKLKDVQEQLSRLKDEEISAAAIKAQFEKQLLTERTLKTQAVNKLA
EIMNRKEPVKRGNDTDVRRKEKENRKLHMELKSEREKLTQQMIKYQKELN
EMQAQIAEESQIRIELQMTLDSKDSDIEQLRSQLQALHIGLDSSSIGSGP
GDAEADDGFPESRLEGWLSLPVRNNTKKFGWVKKYVIVSSKKILFYDSEQ
DKEQSNPYMVLDIDKLFHVRPVTQTDVYRADAKEIPRIFQILYANEGESK
KEQEFPVEPVGEKSNYICHKGHEFIPTLYHFPTNCEACMKPLWHMFKPPP
ALECRRCHIKCHKDHMDKKEEIIAPCKVYYDISTAKNLLLLANSTEEQQK
WVSRLVKKIPKKPPAPDPFARSSPRTSMKIQQNQSIRRPSRQLAPNKPS*

• Vector Type: Mammalian
• Antibiotic: Amp
• DU Number: DU14364
• Unit Source: Protein Phosphorylation and Ubiquitylation Unit
• Location: PPU
• MTA: Dundee
• Notes: N ter FLAG for mammalian expression.; ; Estimated MW of expressed protein : 162165 Daltons