cDNA Clone USP14

• Plasmid: pET156P USP14
• Parent Plasmid: pET15b 6His 3C
• Expressed: 6His-USP14
• Genbank: AAH03556.1
• Tag: 6His
• Species: Human

Sequence of Insert

GGATCCATGCCGCTCTACTCCGTTACTGTAAAATGGGGAAAGGAGAAATT
TGAAGGTGTAGAATTGAATACAGATGAACCTCCAATGGTATTCAAGGCTC
AGCTGTTTGCGTTGACTGGAGTCCAGCCTGCCAGACAGAAAGTTATGGTG
AAAGGAGGAACGCTAAAGGATGATGATTGGGGAAACATCAAAATAAAAAA
TGGAATGACTCTACTAATGATGGGGTCAGCAGATGCTCTTCCAGAAGAAC
CCTCAGCCAAAACTGTCTTCGTAGAAGACATGACAGAAGAACAGTTAGCA
TCTGCTATGGAGTTACCATGTGGATTGACAAACCTTGGTAACACTTGTTA
CATGAATGCCACAGTTCAGTGTATTCGTTCTGTGCCTGAACTCAAAGATG
CCCTTAAAAGGTATGCAGGTGCCTTGAGAGCTTCAGGGGAAATGGCTTCA
GCGCAGTATATTACTGCAGCCCTTAGAGATTTGTTTGATTCCATGGATAA
AACTTCTTCCAGTATTCCACCTATTATTCTACTGCAGTTTTTGCACATGG
CTTTCCCACAGTTTGCCGAGAAAGGTGAACAAGGACAGTATCTTCAACAG
GATGCTAATGAATGTTGGATACAAATGATGCGAGTATTGCAACAGAAATT
GGAAGCAATAGAGGATGATTCTGTTAAAGAGACAGACTCCTCATCTGCAT
CGGCAGCGACACCTTCTAAAAAGAAAAGTTTAATCGATCAGTTCTTCGGT
GTTGAGTTTGAAACTACCATGAAATGTACAGAATCTGAAGAAGAAGAAGT
CACCAAAGGAAAGGAAAATCAACTTCAGCTTAGCTGTTTTATCAATCAGG
AAGTCAAGTATCTTTTTACAGGACTTAAATTGCGACTTCAGGAAGAAATC
ACCAAACAGTCTCCAACGTTGCAAAGAAATGCCTTGTATATCAAATCTTC
CAAGATCAGCCGGCTGCCTGCTTACTTGACCATTCAGATGGTTCGATTTT
TTTATAAAGAGAAGGAATCTGTGAATGCCAAAGTTCTTAAGGATGTTAAA
TTTCCTCTTATGTTGGATATGTATGAACTGTGTACACCAGAACTTCAAGA
GAAAATGGTGTCTTTTCGATCCAAATTCAAGGATCTAGAAGATAAAAAAG
TGAATCAGCAGCCAAATACAAGTGACAAAAAGAGTAGTCCCCAGAAAGAA
GTTAAGTATGAACCCTTTTCTTTTGCTGATGATATTGGCTCCAATAATTG
TGGATACTATGACTTACAAGCAGTACTAACACACCAGGGAAGGTCTAGTT
CTTCAGGTCATTATGTATCATGGGTGAAAAGGAAACAAGATGAATGGATT
AAGTTTGATGATGACAAAGTCAGCATCGTAACACCAGAAGATATCTTACG
GCTTTCTGGTGGTGGAGACTGGCATATCGCTTACGTTCTACTCTATGGGC
CTCGCAGAGTTGAAATAATGGAAGAGGAAAGTGAACAGTAAGCGGCCGC

Amino Acid Sequence

MGSSHHHHHHSSGLEVLFQGPGSMPLYSVTVKWGKEKFEGVELNTDEPPM
VFKAQLFALTGVQPARQKVMVKGGTLKDDDWGNIKIKNGMTLLMMGSADA
LPEEPSAKTVFVEDMTEEQLASAMELPCGLTNLGNTCYMNATVQCIRSVP
ELKDALKRYAGALRASGEMASAQYITAALRDLFDSMDKTSSSIPPIILLQ
FLHMAFPQFAEKGEQGQYLQQDANECWIQMMRVLQQKLEAIEDDSVKETD
SSSASAATPSKKKSLIDQFFGVEFETTMKCTESEEEEVTKGKENQLQLSC
FINQEVKYLFTGLKLRLQEEITKQSPTLQRNALYIKSSKISRLPAYLTIQ
MVRFFYKEKESVNAKVLKDVKFPLMLDMYELCTPELQEKMVSFRSKFKDL
EDKKVNQQPNTSDKKSSPQKEVKYEPFSFADDIGSNNCGYYDLQAVLTHQ
GRSSSSGHYVSWVKRKQDEWIKFDDDKVSIVTPEDILRLSGGGDWHIAYV
LLYGPRRVEIMEEESEQ*

• Vector Type: Bacterial
• Antibiotic: Amp
• DU Number: DU14352
• Unit Source: Protein Phosphorylation and Ubiquitylation Unit
• Location: PPU
• MTA: Dundee
• Notes: N ter 6HIS for bacterial expression.; ; Estimated MW of expressed protein : 58512 Daltons