cDNA Clone NCC

• Plasmid: pCMV5-FLAG SLC12A3 (hNCC) T55A T60A
• Parent Plasmid: pSG5
• Expressed: FLAG-NCC T55A T60A
• Genbank: P55017
• Tag: FLAG
• Species: Human

Sequence of Insert

GCCACCATGGACTACAAGGACGATGACGATAAGGGATCCATGGCAGAACT
GCCCACAACAGAGACGCCTGGGGACGCCACTTTGTGCAGCGGGCGCTTCA
CCATCAGCACACTGCTGAGCAGTGATGAGCCCTCTCCACCAGCTGCCTAT
GACAGCAGCCACCCCAGCCACCTGACCCACAGCAGCACCTTCTGCATGCG
CGCCTTTGGCTATAACGCGATCGATGTGGTGCCCACATATGAGCACTATG
CCAACAGCACCCAGCCTGGTGAGCCCCGGAAGGTCCGGCCCACACTGGCT
GACCTGCACTCCTTCCTCAAGCAGGAAGGCAGACACCTGCATGCCCTGGC
CTTTGACAGCCGGCCCAGCCACGAGATGACTGATGGGCTGGTGGAGGGCG
AGGCAGGCACCAGCAGCGAGAAGAACCCCGAGGAGCCAGTGCGCTTCGGC
TGGGTCAAGGGGGTGATGATTCGTTGCATGCTCAACATTTGGGGCGTGAT
CCTCTACCTGCGGCTGCCCTGGATTACGGCCCAGGCAGGCATCGTCCTGA
CCTGGATCATCATCCTGCTGTCGGTCACGGTGACCTCCATCACAGGCCTC
TCCATCTCAGCCATCTCCACCAATGGCAAGGTCAAGTCAGGTGGCACCTA
CTTCCTCATCTCCCGGAGTCTGGGCCCAGAGCTTGGGGGCTCCATCGGCC
TCATTTTCGCTTTCGCCAATGCCGTGGGTGTGGCCATGCACACGGTGGGC
TTTGCAGAGACCGTGCGGGACCTGCTCCAGGAGTATGGGGCACCCATCGT
GGACCCCATTAACGACATCCGCATCATTGGCGTGGTCTCGGTCACTGTGC
TGCTGGCCATCTCCCTGGCTGGCATGGAGTGGGAGTCCAAGGCCCAGGTG
CTGTTCTTCCTTGTCATCATGGTCTCCTTTGCCAACTATTTAGTGGGGAC
GCTGATCCCCCCATCTGAGGACAAGGCCTCCAAAGGCTTCTTCAGCTACC
GGGCGGACATTTTTGTCCAAAACTTGGTGCCTGACTGGCGGGGTCCAGAT
GGCACCTTCTTCGGAATGTTCTCCATCTTCTTCCCCTCGGCCACAGGCAT
CCTGGCAGGGGCCAACATATCTGGTGACCTCAAGGACCCTGCTATAGCCA
TCCCCAAGGGGACCCTCATGGCCATTTTCTGGACGACCATTTCCTACCTG
GCCATCTCAGCCACCATTGGCTCCTGCGTGGTGCGTGATGCCTCTGGGGT
CCTGAATGACACAGTGACCCCTGGCTGGGGTGCCTGCGAGGGGCTGGCCT
GCAGCTATGGCTGGAACTTCACCGAGTGCACCCAGCAGCACAGCTGCCAC
TACGGCCTCATCAACTATTACCAGACCATGAGCATGGTGTCAGGCTTCGC
GCCCCTGATCACGGCTGGCATCTTCGGGGCCACCCTCTCCTCTGCCCTGG
CCTGCCTTGTCTCTGCTGCCAAAGTCTTCCAGTGCCTTTGCGAGGACCAG
CTGTACCCACTGATCGGCTTCTTCGGCAAAGGCTATGGCAAGAACAAGGA
GCCCGTGCGTGGCTACCTGCTGGCCTACGCCATCGCTGTGGCCTTCATCA
TCATCGCTGAGCTCAACACCATAGCCCCCATCATTTCCAACTTCTTCCTC
TGCTCCTATGCCCTCATCAACTTCAGCTGCTTCCACGCCTCCATCACCAA
CTCGCCTGGGTGGAGACCTTCATTCCAATACTACAACAAGTGGGCGGCGC
TGTTTGGGGCTATCATCTCCGTGGTCATCATGTTCCTCCTCACCTGGTGG
GCGGCCCTCATCGCCATTGGCGTGGTGCTCTTCCTCCTGCTCTATGTCAT
CTACAAGAAGCCAGAGGTAAATTGGGGCTCCTCGGTACAGGCTGGCTCCT
ACAACCTGGCCCTCAGCTACTCGGTGGGCCTCAATGAGGTGGAAGACCAC
ATCAAGAACTACCGCCCCCAGTGCCTGGTGCTCACGGGGCCCCCCAACTT
CCGCCCGGCCCTGGTGGACTTTGTGGGCACCTTCACCCGGAACCTCAGCC
TGATGATCTGTGGCCACGTGCTCATCGGACCCCACAAGCAGAGGATGCCT
GAGCTCCAGCTCATCGCCAACGGGCACACCAAGTGGCTGAACAAGAGGAA
GATCAAGGCCTTCTACTCGGATGTCATTGCTGAGGACCTCCGCAGAGGCG
TCCAGATCCTCATGCAGGCCGCAGGTCTCGGGAGAATGAAGCCCAACATT
CTGGTGGTTGGGTTCAAGAAGAACTGGCAGTCGGCTCACCCGGCCACAGT
GGAAGACTACATTGGCATCCTCCATGATGCCTTTGATTTCAACTATGGCG
TGTGTGTCATGAGGATGCGGGAGGGACTCAACGTGTCCAAGATGATGCAG
GCGCACATTAACCCCGTGTTTGACCCAGCGGAGGACGGGAAGGAAGCCAG
CGCCAGAGTGGACCCCAAGGCCCTGGTGAAGGAGGAGCAGGCCACCACCA
TCTTCCAGTCGGAGCAGGGCAAGAAGACCATAGACATCTACTGGCTCTTT
GACGATGGAGGCCTCACCCTCCTCATTCCCTATCTCCTTGGCCGCAAGAG
GAGGTGGAGCAAATGCAAGATCCGTGTGTTCGTAGGTGGCCAGATTAACA
GGATGGACCAGGAGAGAAAGGCGATCATTTCTCTGCTGAGCAAGTTCCGA
CTGGGATTCCATGAAGTCCACATCCTCCCTGACATCAACCAGAACCCTCG
GGCTGAGCACACCAAGAGGTTTGAGGACATGATTGCACCCTTCCGTCTGA
ATGATGGCTTCAAGGATGAGGCCACTGTCAACGAGATGCGGCGGGACTGC
CCCTGGAAGATCTCAGATGAGGAGATTACGAAGAACAGAGTCAAGTCCCT
TCGGCAGGTGAGGCTGAATGAGATTGTGCTGGATTACTCCCGAGACGCTG
CTCTCATCGTCATCACTTTGCCCATAGGGAGGAAGGGGAAGTGCCCCAGC
TCGCTGTACATGGCCTGGCTGGAGACCCTGTCCCAGGACCTCAGACCTCC
AGTCATCCTGATCCGAGGAAACCAGGAAAACGTGCTCACCTTTTACTGCC
AGTAAGCGGCCGCG

Amino Acid Sequence

MDYKDDDDKGSMAELPTTETPGDATLCSGRFTISTLLSSDEPSPPAAYDS
SHPSHLTHSSTFCMRAFGYNAIDVVPTYEHYANSTQPGEPRKVRPTLADL
HSFLKQEGRHLHALAFDSRPSHEMTDGLVEGEAGTSSEKNPEEPVRFGWV
KGVMIRCMLNIWGVILYLRLPWITAQAGIVLTWIIILLSVTVTSITGLSI
SAISTNGKVKSGGTYFLISRSLGPELGGSIGLIFAFANAVGVAMHTVGFA
ETVRDLLQEYGAPIVDPINDIRIIGVVSVTVLLAISLAGMEWESKAQVLF
FLVIMVSFANYLVGTLIPPSEDKASKGFFSYRADIFVQNLVPDWRGPDGT
FFGMFSIFFPSATGILAGANISGDLKDPAIAIPKGTLMAIFWTTISYLAI
SATIGSCVVRDASGVLNDTVTPGWGACEGLACSYGWNFTECTQQHSCHYG
LINYYQTMSMVSGFAPLITAGIFGATLSSALACLVSAAKVFQCLCEDQLY
PLIGFFGKGYGKNKEPVRGYLLAYAIAVAFIIIAELNTIAPIISNFFLCS
YALINFSCFHASITNSPGWRPSFQYYNKWAALFGAIISVVIMFLLTWWAA
LIAIGVVLFLLLYVIYKKPEVNWGSSVQAGSYNLALSYSVGLNEVEDHIK
NYRPQCLVLTGPPNFRPALVDFVGTFTRNLSLMICGHVLIGPHKQRMPEL
QLIANGHTKWLNKRKIKAFYSDVIAEDLRRGVQILMQAAGLGRMKPNILV
VGFKKNWQSAHPATVEDYIGILHDAFDFNYGVCVMRMREGLNVSKMMQAH
INPVFDPAEDGKEASARVDPKALVKEEQATTIFQSEQGKKTIDIYWLFDD
GGLTLLIPYLLGRKRRWSKCKIRVFVGGQINRMDQERKAIISLLSKFRLG
FHEVHILPDINQNPRAEHTKRFEDMIAPFRLNDGFKDEATVNEMRRDCPW
KISDEEITKNRVKSLRQVRLNEIVLDYSRDAALIVITLPIGRKGKCPSSL
YMAWLETLSQDLRPPVILIRGNQENVLTFYCQ*

• Vector Type: Mammalian
• Antibiotic: Amp
• DU Number: DU4942
• Unit Source: Protein Phosphorylation and Ubiquitylation Unit
• Location: PPU
• MTA: Dundee
• Notes: Mammalian expression with FLAG tag.