cDNA Clone ROCK2

• Plasmid: pFBHTb ROCK2 1-542 L47Q E418G
• Parent Plasmid: pFastBac HTb
• Expressed: His-ROCK2 1-542 L47Q E418G
• Genbank: NM_004850
• Tag: 6His
• Species: Human

Sequence of Insert

ATGTCGTACTACCATCACCATCACCATCACGATTACGATATCCCAACGAC
CGAAAACCTGTATTTTCAGGGCGCCATGGGATCCATGAGCCGGCCCCCGC
CGACGGGGAAAATGCCCGGCGCCCCCGAGACCGCGCCGGGGGACGGGGCA
GGCGCGAGCCGCCAGAGGAAGCTGGAGGCGCTGATCCGAGACCCTCGCTC
CCCCATCAACGTGGAGAGCTTGCAGGATGGCTTAAATTCCTTGGTCCTTG
ATTTAGATTTTCCTGCTTTGAGGAAAAACAAGAACATAGATAATTTCTTA
AATAGATATGAGAAAATTGTGAAAAAAATCAGAGGTCTACAGATGAAGGC
AGAAGACTATGATGTTGTAAAAGTTATTGGAAGAGGTGCTTTTGGTGAAG
TGCAGTTGGTTCGTCACAAGGCATCGCAGAAGGTTTATGCTATGAAGCTT
CTTAGTAAGTTTGAAATGATAAAAAGATCAGATTCTGCCTTTTTTTGGGA
AGAAAGAGATATTATGGCCTTTGCCAATAGCCCCTGGGTGGTTCAGCTTT
TTTATGCCTTTCAAGATGATAGGTATCTGTACATGGTAATGGAGTACATG
CCTGGTGGAGACCTTGTAAACCTTATGAGTAATTATGATGTGCCTGAAAA
ATGGGCCAAATTTTACACTGCTGAAGTTGTTCTTGCTCTGGATGCAATAC
ACTCCATGGGTTTAATACACAGAGATGTGAAGCCTGACAACATGCTCTTG
GATAAACATGGACATCTAAAATTAGCAGATTTTGGCACGTGCATGAAGAT
GGATGAAACAGGCATGGTACATTGTGATACAGCAGTTGGAACACCGGATT
ATATATCACCTGAGGTTCTGAAATCACAAGGGGGTGATGGTTTCTATGGG
CGAGAATGTGATTGGTGGTCTGTAGGTGTTTTCCTTTATGAGATGCTAGT
GGGGGATACTCCATTTTATGCGGATTCACTTGTAGGAACATATAGCAAAA
TTATGGATCATAAGAATTCACTGTGTTTCCCTGAAGATGCAGAAATTTCC
AAACATGCAAAGAATCTCATCTGTGCTTTCTTAACAGATAGGGAGGTACG
ACTTGGGAGAAATGGGGTGGAAGAAATCAGACAGCATCCTTTCTTTAAGA
ATGATCAGTGGCATTGGGATAACATAAGAGAAACGGCAGCTCCTGTAGTA
CCTGAACTCAGCAGTGACATAGACAGCAGCAATTTCGATGACATTGAAGA
TGACAAAGGAGATGTAGAAACCTTCCCAATTCCTAAAGCTTTTGTTGGAA
ATCAGCTGCCTTTCATCGGATTTACCTACTATAGAGGAAATTTATTATTA
AGTGACTCTCCATCTTGTAGAGAAACTGATTCCATACAATCAAGGAAAAA
TGAAGAAAGTCAAGAGATTCAGAAAAAACTGTATACATTAGAAGAACATC
TTAGCAATGAGATGCAAGCCAAAGAGGAACTGGAACAGAAGTGCAAATCT
GTTAATACTCGCCTAGAAAAAACAGCAAAGGAGCTAGAAGAGGAGATTAC
CTTACGGAAAAGTGTGGAATCAGCATTAAGACAGTTAGAAAGAGAAAAGG
CGCTTCTTCAGCACAAAAATGCAGAATATCAGAGGAAAGCTGATCATGAA
GCAGACAAAAAACGAAATTTGGAAAATGATGTTAACAGCTTAAAAGATCA
ACTTGAAGATTTGAAAAAAAGAAATCAAAACTCTCAAATATAAGCGGCCG
C

Amino Acid Sequence

MSYYHHHHHHDYDIPTTENLYFQGAMGSMSRPPPTGKMPGAPETAPGDGA
GASRQRKLEALIRDPRSPINVESLQDGLNSLVLDLDFPALRKNKNIDNFL
NRYEKIVKKIRGLQMKAEDYDVVKVIGRGAFGEVQLVRHKASQKVYAMKL
LSKFEMIKRSDSAFFWEERDIMAFANSPWVVQLFYAFQDDRYLYMVMEYM
PGGDLVNLMSNYDVPEKWAKFYTAEVVLALDAIHSMGLIHRDVKPDNMLL
DKHGHLKLADFGTCMKMDETGMVHCDTAVGTPDYISPEVLKSQGGDGFYG
RECDWWSVGVFLYEMLVGDTPFYADSLVGTYSKIMDHKNSLCFPEDAEIS
KHAKNLICAFLTDREVRLGRNGVEEIRQHPFFKNDQWHWDNIRETAAPVV
PELSSDIDSSNFDDIEDDKGDVETFPIPKAFVGNQLPFIGFTYYRGNLLL
SDSPSCRETDSIQSRKNEESQEIQKKLYTLEEHLSNEMQAKEELEQKCKS
VNTRLEKTAKELEEEITLRKSVESALRQLEREKALLQHKNAEYQRKADHE
ADKKRNLENDVNSLKDQLEDLKKRNQNSQI*

• Vector Type: Baculovirus
• Antibiotic: Amp
• DU Number: DU4817
• Unit Source: Protein Phosphorylation and Ubiquitylation Unit
• Location: PPU
• MTA: Dundee
• Notes: Baculovirus expression with N term 6H.