cDNA Clone NCC

• Plasmid: pNEBRX1 Hygro FLAG SLC12A3 (hNCC)
• Parent Plasmid: pNEBRX1
• Expressed: FLAG-SLC12A3 (hNCC)
• Genbank: P55017
• Tag: FLAG
• Species: Human

Sequence of Insert

CACATCGGGGCGGGGATATTGTCCCTCCGAGCGGAGTACTGTCCTCCGAG
CGGAGTACTGTCCTCCGAGCGGAGTACTGTCCTCCGAGCGGAGTTCTGTC
CTCCGAGCGGAGACTCTAGAGGGTATATAATGGGGGCCGTCAGCTACTAC
CAGAGCTCATGGGCCCGTGCAATTGAAGCCGGCTGGCGCCAAGCTTCTCT
GCAGGATATCTGGATCCGCCACCATGGACTACAAGGACGACGATGACAAG
ATGGCAGAACTGCCCACAACAGAGACGCCTGGGGACGCCACTTTGTGCAG
CGGGCGCTTCACCATCAGCACACTGCTGAGCAGTGATGAGCCCTCTCCAC
CAGCTGCCTATGACAGCAGCCACCCCAGCCACCTGACCCACAGCAGCACC
TTCTGCATGCGCACCTTTGGCTACAACACGATCGATGTGGTGCCCACATA
TGAGCACTATGCCAACAGCACCCAGCCTGGTGAGCCCCGGAAGGTCCGGC
CCACACTGGCTGACCTGCACTCCTTCCTCAAGCAGGAAGGCAGACACCTG
CATGCCCTGGCCTTTGACAGCCGGCCCAGCCACGAGATGACTGATGGGCT
GGTGGAGGGCGAGGCAGGCACCAGCAGCGAGAAGAACCCCGAGGAGCCAG
TGCGCTTCGGCTGGGTCAAGGGGGTGATGATTCGTTGCATGCTCAACATT
TGGGGCGTGATCCTCTACCTGCGGCTGCCCTGGATTACGGCCCAGGCAGG
CATCGTCCTGACCTGGATCATCATCCTGCTGTCGGTCACGGTGACCTCCA
TCACAGGCCTCTCCATCTCAGCCATCTCCACCAATGGCAAGGTCAAGTCA
GGTGGCACCTACTTCCTCATCTCCCGGAGTCTGGGCCCAGAGCTTGGGGG
CTCCATCGGCCTCATTTTCGCTTTCGCCAATGCCGTGGGTGTGGCCATGC
ACACGGTGGGCTTTGCAGAGACCGTGCGGGACCTGCTCCAGGAGTATGGG
GCACCCATCGTGGACCCCATTAACGACATCCGCATCATTGGCGTGGTCTC
GGTCACTGTGCTGCTGGCCATCTCCCTGGCTGGCATGGAGTGGGAGTCCA
AGGCCCAGGTGCTGTTCTTCCTTGTCATCATGGTCTCCTTTGCCAACTAT
TTAGTGGGGACGCTGATCCCCCCATCTGAGGACAAGGCCTCCAAAGGCTT
CTTCAGCTACCGGGCGGACATTTTTGTCCAAAACTTGGTGCCTGACTGGC
GGGGTCCAGATGGCACCTTCTTCGGAATGTTCTCCATCTTCTTCCCCTCG
GCCACAGGCATCCTGGCAGGGGCCAACATATCTGGTGACCTCAAGGACCC
TGCTATAGCCATCCCCAAGGGGACCCTCATGGCCATTTTCTGGACGACCA
TTTCCTACCTGGCCATCTCAGCCACCATTGGCTCCTGCGTGGTGCGTGAT
GCCTCTGGGGTCCTGAATGACACAGTGACCCCTGGCTGGGGTGCCTGCGA
GGGGCTGGCCTGCAGCTATGGCTGGAACTTCACCGAGTGCACCCAGCAGC
ACAGCTGCCACTACGGCCTCATCAACTATTACCAGACCATGAGCATGGTG
TCAGGCTTCGCGCCCCTGATCACGGCTGGCATCTTCGGGGCCACCCTCTC
CTCTGCCCTGGCCTGCCTTGTCTCTGCTGCCAAAGTCTTCCAGTGCCTTT
GCGAGGACCAGCTGTACCCACTGATCGGCTTCTTCGGCAAAGGCTATGGC
AAGAACAAGGAGCCCGTGCGTGGCTACCTGCTGGCCTACGCCATCGCTGT
GGCCTTCATCATCATCGCTGAGCTCAACACCATAGCCCCCATCATTTCCA
ACTTCTTCCTCTGCTCCTATGCCCTCATCAACTTCAGCTGCTTCCACGCC
TCCATCACCAACTCGCCTGGGTGGAGACCTTCATTCCAATACTACAACAA
GTGGGCGGCGCTGTTTGGGGCTATCATCTCCGTGGTCATCATGTTCCTCC
TCACCTGGTGGGCGGCCCTCATCGCCATTGGCGTGGTGCTCTTCCTCCTG
CTCTATGTCATCTACAAGAAGCCAGAGGTAAATTGGGGCTCCTCGGTACA
GGCTGGCTCCTACAACCTGGCCCTCAGCTACTCGGTGGGCCTCAATGAGG
TGGAAGACCACATCAAGAACTACCGCCCCCAGTGCCTGGTGCTCACGGGG
CCCCCCAACTTCCGCCCGGCCCTGGTGGACTTTGTGGGCACCTTCACCCG
GAACCTCAGCCTGATGATCTGTGGCCACGTGCTCATCGGACCCCACAAGC
AGAGGATGCCTGAGCTCCAGCTCATCGCCAACGGGCACACCAAGTGGCTG
AACAAGAGGAAGATCAAGGCCTTCTACTCGGATGTCATTGCTGAGGACCT
CCGCAGAGGCGTCCAGATCCTCATGCAGGCCGCAGGTCTCGGGAGAATGA
AGCCCAACATTCTGGTGGTTGGGTTCAAGAAGAACTGGCAGTCGGCTCAC
CCGGCCACAGTGGAAGACTACATTGGCATCCTCCATGATGCCTTTGATTT
CAACTATGGCGTGTGTGTCATGAGGATGCGGGAGGGACTCAACGTGTCCA
AGATGATGCAGGCGCACATTAACCCCGTGTTTGACCCAGCGGAGGACGGG
AAGGAAGCCAGCGCCAGAGTGGACCCCAAGGCCCTGGTGAAGGAGGAGCA
GGCCACCACCATCTTCCAGTCGGAGCAGGGCAAGAAGACCATAGACATCT
ACTGGCTCTTTGACGATGGAGGCCTCACCCTCCTCATTCCCTATCTCCTT
GGCCGCAAGAGGAGGTGGAGCAAATGCAAGATCCGTGTGTTCGTAGGTGG
CCAGATTAACAGGATGGACCAGGAGAGAAAGGCGATCATTTCTCTGCTGA
GCAAGTTCCGACTGGGATTCCATGAAGTCCACATCCTCCCTGACATCAAC
CAGAACCCTCGGGCTGAGCACACCAAGAGGTTTGAGGACATGATTGCACC
CTTCCGTCTGAATGATGGCTTCAAGGATGAGGCCACTGTCAACGAGATGC
GGCGGGACTGCCCCTGGAAGATCTCAGATGAGGAGATTACGAAGAACAGA
GTCAAGTCCCTTCGGCAGGTGAGGCTGAATGAGATTGTGCTGGATTACTC
CCGAGACGCTGCTCTCATCGTCATCACTTTGCCCATAGGGAGGAAGGGGA
AGTGCCCCAGCTCGCTGTACATGGCCTGGCTGGAGACCCTGTCCCAGGAC
CTCAGACCTCCAGTCATCCTGATCCGAGGAAACCAGGAAAACGTGCTCAC
CTTTTACTGCCAGTAAGCGGCCGCTAGCGATCGCGGCGCGCCACGCGTCT
CCGGATGTACAGGCATGCGTCGACCCTCTAGTCAAGGCCTATAGTGAGTC
GTATTACGGACTATCGTCGTTTACAACGTCGTGACTGGAAAACCCTGACG
TACCCAACTAATCGCTGCAGCACATCCCCTTCGCAGCTGGCGTAATAGCG
AGAGCGGCACGATCGCCTTCAACAGTGGCCAGCTGATGGGCATGACCTCG
CTGTAATAAGCCGCTCCGGCGGGCCTTTTTGGTA

Amino Acid Sequence

MDYKDDDDKMAELPTTETPGDATLCSGRFTISTLLSSDEPSPPAAYDSSH
PSHLTHSSTFCMRTFGYNTIDVVPTYEHYANSTQPGEPRKVRPTLADLHS
FLKQEGRHLHALAFDSRPSHEMTDGLVEGEAGTSSEKNPEEPVRFGWVKG
VMIRCMLNIWGVILYLRLPWITAQAGIVLTWIIILLSVTVTSITGLSISA
ISTNGKVKSGGTYFLISRSLGPELGGSIGLIFAFANAVGVAMHTVGFAET
VRDLLQEYGAPIVDPINDIRIIGVVSVTVLLAISLAGMEWESKAQVLFFL
VIMVSFANYLVGTLIPPSEDKASKGFFSYRADIFVQNLVPDWRGPDGTFF
GMFSIFFPSATGILAGANISGDLKDPAIAIPKGTLMAIFWTTISYLAISA
TIGSCVVRDASGVLNDTVTPGWGACEGLACSYGWNFTECTQQHSCHYGLI
NYYQTMSMVSGFAPLITAGIFGATLSSALACLVSAAKVFQCLCEDQLYPL
IGFFGKGYGKNKEPVRGYLLAYAIAVAFIIIAELNTIAPIISNFFLCSYA
LINFSCFHASITNSPGWRPSFQYYNKWAALFGAIISVVIMFLLTWWAALI
AIGVVLFLLLYVIYKKPEVNWGSSVQAGSYNLALSYSVGLNEVEDHIKNY
RPQCLVLTGPPNFRPALVDFVGTFTRNLSLMICGHVLIGPHKQRMPELQL
IANGHTKWLNKRKIKAFYSDVIAEDLRRGVQILMQAAGLGRMKPNILVVG
FKKNWQSAHPATVEDYIGILHDAFDFNYGVCVMRMREGLNVSKMMQAHIN
PVFDPAEDGKEASARVDPKALVKEEQATTIFQSEQGKKTIDIYWLFDDGG
LTLLIPYLLGRKRRWSKCKIRVFVGGQINRMDQERKAIISLLSKFRLGFH
EVHILPDINQNPRAEHTKRFEDMIAPFRLNDGFKDEATVNEMRRDCPWKI
SDEEITKNRVKSLRQVRLNEIVLDYSRDAALIVITLPIGRKGKCPSSLYM
AWLETLSQDLRPPVILIRGNQENVLTFYCQ*

• Vector Type: Other
• Antibiotic: Amp
• DU Number: DU4630
• Unit Source: Protein Phosphorylation and Ubiquitylation Unit
• Location: PPU
• MTA: Dundee
• Notes: Mammalian expression with N term FLAG.