cDNA Clone UBCH5a

• Synonyms: x
• Plasmid: pET28a(+) UBE2D1 (UBCH5a)
• Parent Plasmid: pET28a original
• Expressed: His-UBE2D1 (UBCH5a)
• Genbank: NM_003338 Gene 7321
• Tag: 6His
• Species: Human

Sequence of Insert

ATGGGCAGCAGCCATCATCATCATCATCACAGCAGCGGCCTGGTGCCGCG
CGGCAGCCATATGGCTAGCATGACTGGTGGACAGCAAATGGGTCGCGGAT
CCGCGCTGAAGAGGATTCAGAAAGAATTGAGTGATCTACAGCGCGATCCA
CCTGCTCACTGTTCAGCTGGACCTGTGGGAGATGACTTGTTCCACTGGCA
AGCCACTATTATGGGGCCTCCTGATAGCGCATATCAAGGTGGAGTCTTCT
TTCTCACTGTACATTTTCCGACAGATTATCCTTTTAAACCACCAAAGATT
GCTTTCACAACAAAAATTTACCATCCAAACATAAACAGTAATGGAAGTAT
TTGTCTCGATATTCTGAGGTCACAATGGTCACCAGCTCTGACTGTATCAA
AAGTTTTATTGTCCATATGTTCTCTACTTTGTGATCCTAATCCAGATGAC
CCCTTAGTACCAGATATTGCACAAATCTATAAATCAGACAAAGAAAAATA
CAACAGACATGCAAGAGAATGGACTCAGAAATATGCAATGTAAGCGGCCG
C

Amino Acid Sequence

MGSSHHHHHHSSGLVPRGSHMASMTGGQQMGRGSALKRIQKELSDLQRDP
PAHCSAGPVGDDLFHWQATIMGPPDSAYQGGVFFLTVHFPTDYPFKPPKI
AFTTKIYHPNINSNGSICLDILRSQWSPALTVSKVLLSICSLLCDPNPDD
PLVPDIAQIYKSDKEKYNRHAREWTQKYAM*

• Vector Type: Bacterial
• Antibiotic: Kan
• Full Snapgene Map: pET28a(+) UBE2D1 (UBCH5a)47.1 KB Snapgene files can be opened using software available here: www.snapgene.com
• DU Number: DU4315
• Unit Source: Protein Phosphorylation and Ubiquitylation Unit
• Location: PPU
• MTA: Dundee
• Notes: Bacterial expression with N term 6H.; isoform 1
• Low Temp: No