cDNA Clone DAZAP1-b

• Plasmid: pEBG6P-1 Dazap1-b T315D
• Parent Plasmid: pEBG6P
• Expressed: GST-DAZAP1-b T315D
• Genbank: NM_018959
• Tag: GST
• Species: Human

Sequence of Insert

ATGTCCCCTATACTAGGTTATTGGAAAATTAAGGGCCTTGTGCAACCCAC
TCGACTTCTTTTGGAATATCTTGAAGAAAAATATGAAGAGCATTTGTATG
AGCGCGATGAAGGTGATAAATGGCGAAACAAAAAGTTTGAATTGGGTTTG
GAGTTTCCCAATCTTCCTTATTATATTGATGGTGATGTTAAATTAACACA
GTCTATGGCCATCATACGTTATATAGCTGACAAGCACAACATGTTGGGTG
GTTGTCCAAAAGAGCGTGCAGAGATTTCAATGCTTGAAGGAGCGGTTTTG
GATATTAGATACGGTGTTTCGAGAATTGCATATAGTAAAGACTTTGAAAC
TCTCAAAGTTGATTTTCTTAGCAAGCTACCTGAAATGCTGAAAATGTTCG
AAGATCGTTTATGTCATAAAACATATTTAAATGGTGATCATGTAACCCAT
CCTGACTTCATGTTGTATGACGCTCTTGATGTTGTTTTATACATGGACCC
AATGTGCCTGGATGCGTTCCCAAAATTAGTTTGTTTTAAAAAACGTATTG
AAGCTATCCCACAAATTGATAAGTACTTGAAATCCAGCAAGTATATAGCA
TGGCCTTTGCAGGGCTGGCAAGCCACGTTTGGTGGTGGCGACCATCCTCC
AAAATCGGATCTGGTTCCGCGTGGATCTCTGGAAGTTCTGTTCCAGGGGC
CCCTGGGATCCAACAACTCGGGCGCCGACGAGATCGGGAAGCTCTTCGTG
GGCGGTCTTGACTGGAGCACGACCCAAGAGACTCTGCGCAGCTACTTTTC
CCAATATGGAGAAGTCGTAGATTGTGTTATCATGAAAGATAAAACCACCA
ACCAGTCTCGAGGCTTTGGGTTTGTCAAATTTAAAGACCCAAACTGTGTG
GGGACGGTGCTGGCCAGCAGACCGCACACGCTAGATGGCCGAAACATCGA
CCCCAAGCCATGCACACCCCGGGGGATGCAGCCGGAGAGAACACGGCCGA
AGGAAGGATGGCAGAAAGGACCCAGGAGCGATAACAGTAAATCAAATAAG
ATATTTGTCGGTGGAATTCCTCACAATTGTGGTGAGACAGAGCTCAGGGA
ATACTTCAAGAAGTTCGGAGTGGTCACGGAGGTAGTCATGATCTATGACG
CCGAGAAGCAGAGGCCCCGAGGTTTTGGATTTATTACTTTCGAGGACGAA
CAATCAGTGGACCAGGCTGTCAACATGCATTTTCACGACATCATGGGCAA
AAAAGTGGAAGTTAAACGAGCTGAGCCTCGGGACAGCAAGAGCCAAGCGC
CGGGACAGCCAGGTGCCAGCCAGTGGGGGAGCCGGGTTGTGCCCAACGCT
GCCAATGGCTGGGCAGGCCAGCCCCCGCCCACGTGGCAGCAAGGATATGG
CCCGCAAGGAATGTGGGTGCCGGCAGGACAGGCGATTGGTGGCTATGGAC
CGCCCCCTGCAGGAAGAGGAGCCCCCCCGCCACCCCCACCGTTCACCTCC
TACATCGTGTCCACCCCTCCTGGAGGCTTTCCCCCTCCCCAGGGCTTCCC
TCAGGGCTACGGTGCCCCGCCACAGTTCAGTTTTGGCTACGGGCCTCCAC
CTCCACCGCCAGATCAGTTTGCCCCTCCGGGGGTTCCTCCTCCACCAGCC
GCTCCCGGGGCAGCACCTCTGGCTTTCCCACCGCCTCCGTCTCAGGCTGC
CCCGGACATGAGCAAGCCCCCGACAGCTCAGCCAGACTTCCCCTATGGTC
AGTATGCAGGTTACGGGCAGGACTTGAGTGGCTTCGGACAGGGCTTCTCA
GACCCCAGCCAGCAGCCTCCTTCCTACGGGGGTCCCTCCGTGCCAGGGTC
GGGGGGCCCCCCCGCCGGCGGCAGCGGCTTTGGACGAGGGCAGAACCACA
ACGTGCAAGGGTTCCACCCCTACCGACGCTAGGCGGCCGCGACTCTAGAG
TGAGGGTCCCCACCTGGGACCCTTGAGAGTATCAGGTCTCCCACGTGGGA
GACAAGAAATCCCTGTTTAATATAAGAGTCGGCCC

Amino Acid Sequence

MSPILGYWKIKGLVQPTRLLLEYLEEKYEEHLYERDEGDKWRNKKFELGL
EFPNLPYYIDGDVKLTQSMAIIRYIADKHNMLGGCPKERAEISMLEGAVL
DIRYGVSRIAYSKDFETLKVDFLSKLPEMLKMFEDRLCHKTYLNGDHVTH
PDFMLYDALDVVLYMDPMCLDAFPKLVCFKKRIEAIPQIDKYLKSSKYIA
WPLQGWQATFGGGDHPPKSDLVPRGSLEVLFQGPLGSNNSGADEIGKLFV
GGLDWSTTQETLRSYFSQYGEVVDCVIMKDKTTNQSRGFGFVKFKDPNCV
GTVLASRPHTLDGRNIDPKPCTPRGMQPERTRPKEGWQKGPRSDNSKSNK
IFVGGIPHNCGETELREYFKKFGVVTEVVMIYDAEKQRPRGFGFITFEDE
QSVDQAVNMHFHDIMGKKVEVKRAEPRDSKSQAPGQPGASQWGSRVVPNA
ANGWAGQPPPTWQQGYGPQGMWVPAGQAIGGYGPPPAGRGAPPPPPPFTS
YIVSTPPGGFPPPQGFPQGYGAPPQFSFGYGPPPPPPDQFAPPGVPPPPA
APGAAPLAFPPPPSQAAPDMSKPPTAQPDFPYGQYAGYGQDLSGFGQGFS
DPSQQPPSYGGPSVPGSGGPPAGGSGFGRGQNHNVQGFHPYRR*

• Vector Type: Mammalian
• Antibiotic: Amp
• DU Number: DU4300
• Unit Source: Protein Phosphorylation and Ubiquitylation Unit
• Location: PPU
• MTA: Dundee
• Notes: Mammalian expression with N terminal GST tag.