cDNA Clone DAZAP1-b

• Plasmid: pEBG6P-1 Dazap1-b T269D
• Parent Plasmid: pEBG6P
• Expressed: GST-DAZAP1-b T269D
• Genbank: NM_018959
• Tag: GST
• Species: Human

Sequence of Insert

ATGGCCCCTATACTAGGTTATTGGAAAATTAAGGGCCTTGTGCAACCCAC
TCGACTTCTTTTGGAATATCTTGAAGAAAAATATGAAGAGCATTTGTATG
AGCGCGATGAAGGTGATAAATGGCGAAACAAAAAGTTTGAATTGGGTTTG
GAGTTTCCCAATCTTCCTTATTATATTGATGGTGATGTTAAATTAACACA
GTCTATGGCCATCATACGTTATATAGCTGACAAGCACAACATGTTGGGTG
GTTGTCCAAAAGAGCGTGCAGAGATTTCAATGCTTGAAGGAGCGGTTTTG
GATATTAGATACGGTGTTTCGAGAATTGCATATAGTAAAGACTTTGAAAC
TCTCAAAGTTGATTTTCTTAGCAAGCTACCTGAAATGCTGAAAATGTTCG
AAGATCGTTTATGTCATAAAACATATTTAAATGGTGATCATGTAACCCAT
CCTGACTTCATGTTGTATGACGCTCTTGATGTTGTTTTATACATGGACCC
AATGTGCCTGGATGCGTTCCCAAAATTAGTTTGTTTTAAAAAACGTATTG
AAGCTATCCCACAAATTGATAAGTACTTGAAATCCAGCAAGTATATAGCA
TGGCCTTTGCAGGGCTGGCAAGCCACGTTTGGTGGTGGCGACCATCCTCC
AAAATCGGATCTGGTTCCGCGTGGATCTCTGGAAGTTCTGTTCCAGGGGC
CCCTGGGATCCAACAACTCGGGCGCCGACGAGATCGGGAAGCTCTTCGTG
GGCGGTCTTGACTGGAGCACGACCCAAGAGACTCTGCGCAGCTACTTTTC
CCAATATGGAGAAGTCGTAGATTGTGTTATCATGAAAGATAAAACCACCA
ACCAGTCTCGAGGCTTTGGGTTTGTCAAATTTAAAGACCCAAACTGTGTG
GGGACGGTGCTGGCCAGCAGACCGCACACGCTAGATGGCCGAAACATCGA
CCCCAAGCCATGCACACCCCGGGGGATGCAGCCGGAGAGAACACGGCCGA
AGGAAGGATGGCAGAAAGGACCCAGGAGCGATAACAGTAAATCAAATAAG
ATATTTGTCGGTGGAATTCCTCACAATTGTGGTGAGACAGAGCTCAGGGA
ATACTTCAAGAAGTTCGGAGTGGTCACGGAGGTAGTCATGATCTATGACG
CCGAGAAGCAGAGGCCCCGAGGTTTTGGATTTATTACTTTCGAGGACGAA
CAATCAGTGGACCAGGCTGTCAACATGCATTTTCACGACATCATGGGCAA
AAAAGTGGAAGTTAAACGAGCTGAGCCACGGGACAGCAAGAGCCAAGCGC
CGGGACAGCCAGGTGCCAGCCAGTGGGGGAGCCGGGTTGTGCCCAACGCT
GCCAATGGCTGGGCAGGCCAGCCCCCGCCCACGTGGCAGCAAGGATATGG
CCCGCAAGGAATGTGGGTGCCGGCAGGACAGGCGATTGGTGGCTATGGAC
CGCCCCCTGCAGGAAGAGGAGCCCCCCCGCCACCCCCACCGTTCACCTCC
TACATCGTGTCCGACCCTCCTGGAGGCTTTCCCCCTCCCCAGGGCTTCCC
TCAGGGCTACGGTGCCCCGCCACAGTTCAGTTTTGGCTACGGGCCTCCAC
CTCCACCGCCAGATCAGTTTGCCCCTCCGGGGGTTCCTCCTCCACCAGCC
ACTCCCGGGGCAGCACCTCTGGCTTTCCCACCGCCTCCGTCTCAGGCTGC
CCCGGACATGAGCAAGCCCCCGACAGCTCAGCCAGACTTCCCCTATGGTC
AGTATGCAGGTTACGGGCAGGACTTGAGTGGCTTCGGACAGGGCTTCTCA
GACCCCAGCCAGCAGCCTCCTTCCTACGGGGGTCCCTCCGTGCCAGGGTC
GGGGGGCCCCCCCGCCGGCGGCAGCGGCTTTGGACGAGGGCAGAACCACA
ACGTGCAAGGGTTCCACCCCTACCGACGCTAGGCGGCCGCG

Amino Acid Sequence

MAPILGYWKIKGLVQPTRLLLEYLEEKYEEHLYERDEGDKWRNKKFELGL
EFPNLPYYIDGDVKLTQSMAIIRYIADKHNMLGGCPKERAEISMLEGAVL
DIRYGVSRIAYSKDFETLKVDFLSKLPEMLKMFEDRLCHKTYLNGDHVTH
PDFMLYDALDVVLYMDPMCLDAFPKLVCFKKRIEAIPQIDKYLKSSKYIA
WPLQGWQATFGGGDHPPKSDLVPRGSLEVLFQGPLGSNNSGADEIGKLFV
GGLDWSTTQETLRSYFSQYGEVVDCVIMKDKTTNQSRGFGFVKFKDPNCV
GTVLASRPHTLDGRNIDPKPCTPRGMQPERTRPKEGWQKGPRSDNSKSNK
IFVGGIPHNCGETELREYFKKFGVVTEVVMIYDAEKQRPRGFGFITFEDE
QSVDQAVNMHFHDIMGKKVEVKRAEPRDSKSQAPGQPGASQWGSRVVPNA
ANGWAGQPPPTWQQGYGPQGMWVPAGQAIGGYGPPPAGRGAPPPPPPFTS
YIVSDPPGGFPPPQGFPQGYGAPPQFSFGYGPPPPPPDQFAPPGVPPPPA
TPGAAPLAFPPPPSQAAPDMSKPPTAQPDFPYGQYAGYGQDLSGFGQGFS
DPSQQPPSYGGPSVPGSGGPPAGGSGFGRGQNHNVQGFHPYRR*

• Vector Type: Mammalian
• Antibiotic: Amp
• DU Number: DU4299
• Unit Source: Protein Phosphorylation and Ubiquitylation Unit
• Location: PPU
• MTA: Dundee
• Notes: Mammalian expression with N terminal GST tag.