cDNA Clone BOULE

• Synonyms: BOLL
• Plasmid: pGEX6P-1-BOULE
• Parent Plasmid: pGEX6P1
• Expressed: GST-BOULE
• Genbank: AF272858
• Tag: GST
• Species: Human

Sequence of Insert

ATGTCCCCTATACTAGGTTATTGGAAAATTAAGGGCCTTGTGCAACCCAC
TCGACTTCTTTTGGAATATCTTGAAGAAAAATATGAAGAGCATTTGTATG
AGCGCGATGAAGGTGATAAATGGCGAAACAAAAAGTTTGAATTGGGTTTG
GAGTTTCCCAATCTTCCTTATTATATTGATGGTGATGTTAAATTAACACA
GTCTATGGCCATCATACGTTATATAGCTGACAAGCACAACATGTTGGGTG
GTTGTCCAAAAGAGCGTGCAGAGATTTCAATGCTTGAAGGAGCGGTTTTG
GATATTAGATACGGTGTTTCGAGAATTGCATATAGTAAAGACTTTGAAAC
TCTCAAAGTTGATTTTCTTAGCAAGCTACCTGAAATGCTGAAAATGTTCG
AAGATCGTTTATGTCATAAAACATATTTAAATGGTGATCATGTAACCCAT
CCTGACTTCATGTTGTATGACGCTCTTGATGTTGTTTTATACATGGACCC
AATGTGCCTGGATGCGTTCCCAAAATTAGTTTGTTTTAAAAAACGTATTG
AAGCTATCCCACAAATTGATAAGTACTTGAAATCCAGCAAGTATATAGCA
TGGCCTTTGCAGGGCTGGCAAGCCACGTTTGGTGGTGGCGACCATCCTCC
AAAATCGGATCTGGAAGTTCTGTTCCAGGGGCCCCTGGGATCCCCGGAAT
TCCAAACAGATTCATTATCTCCATCCCCTAATCCTGTGTCACCTGTGCCT
TTGAATAACCCAACAAGTGCCCCAAGATATGGAACAGTGATCCCTAATCG
CATCTTTGTAGGAGGAATTGATTTTAAGACAAACGAAAGTGATTTAAGAA
AATTTTTTTCCCAGTATGGGTCTGTGAAAGAAGTGAAGATTGTAAATGAC
AGAGCTGGAGTATCCAAAGGGTATGGTTTCGTCACTTTTGAAACACAAGA
AGATGCACAAAAAATTTTACAAGAGGCTGAAAAACTTAATTATAAGGATA
AGAAGCTGAACATTGGTCCAGCAATAAGAAAACAACAAGTAGGGATCCCT
CGTTCTAGTATAATGCCAGCAGCTGGAACAATGTATCTAACAACTTCAAC
TGGATATCCTTATACTTACCATAATGGTGTTGCTTATTTTCATACTCCAG
AGGTAACTTCGGTCCCACCGCCTTGGCCTTCACGTTCTGTATGTAGCTCC
CCTGTGATGGTAGCTCAGCCCATTTATCAGCAACCTGCATATCACTACCA
GGCCACCACACAGTATTTACCAGGACAGTGGCAGTGGAGTGTTCCTCAGC
CTTCTGCCTCTTCTGCTCCATTCTTATACCTGCAACCTTCTGAGGTTATT
TATCAACCAGTGGAAATTGCACAGGATGGTGGATGTGTTCCTCCTCCACT
GTCTCTGATGGAAACTTCAGTTCCAGAGCCTTATTCTGATCATGGAGTTC
AAGCAACATATCACCAGGTTTATGCTCCAAGTGCCATCACTATGCCTGCG
CCTGTGATGCAGCCTGAGCCAATTAAAACAGTGTGGAGCATTCATTATTA
AGTCGAC

Amino Acid Sequence

MSPILGYWKIKGLVQPTRLLLEYLEEKYEEHLYERDEGDKWRNKKFELGL
EFPNLPYYIDGDVKLTQSMAIIRYIADKHNMLGGCPKERAEISMLEGAVL
DIRYGVSRIAYSKDFETLKVDFLSKLPEMLKMFEDRLCHKTYLNGDHVTH
PDFMLYDALDVVLYMDPMCLDAFPKLVCFKKRIEAIPQIDKYLKSSKYIA
WPLQGWQATFGGGDHPPKSDLEVLFQGPLGSPEFQTDSLSPSPNPVSPVP
LNNPTSAPRYGTVIPNRIFVGGIDFKTNESDLRKFFSQYGSVKEVKIVND
RAGVSKGYGFVTFETQEDAQKILQEAEKLNYKDKKLNIGPAIRKQQVGIP
RSSIMPAAGTMYLTTSTGYPYTYHNGVAYFHTPEVTSVPPPWPSRSVCSS
PVMVAQPIYQQPAYHYQATTQYLPGQWQWSVPQPSASSAPFLYLQPSEVI
YQPVEIAQDGGCVPPPLSLMETSVPEPYSDHGVQATYHQVYAPSAITMPA
PVMQPEPIKTVWSIHY*

• Vector Type: Bacterial
• Antibiotic: Amp
• DU Number: DU4231
• Unit Source: Protein Phosphorylation and Ubiquitylation Unit
• Location: PPU
• MTA: Dundee
• Notes: Bacterial expression plasmid with N terminal GST tag.