cDNA Clone UBCH2

• Synonyms: UBE2H
• Plasmid: pGEX6P-1-UBCH2
• Parent Plasmid: pGEX6P1
• Expressed: GST-UBCH2
• Genbank: Z29328
• Tag: GST
• Species: Human

Sequence of Insert

ATGTCCCCTATACTAGGTTATTGGAAAATTAAGGGCCTTGTGCAACCCAC
TCGACTTCTTTTGGAATATCTTGAAGAAAAATATGAAGAGCATTTGTATG
AGCGCGATGAAGGTGATAAATGGCGAAACAAAAAGTTTGAATTGGGTTTG
GAGTTTCCCAATCTTCCTTATTATATTGATGGTGATGTTAAATTAACACA
GTCTATGGCCATCATACGTTATATAGCTGACAAGCACAACATGTTGGGTG
GTTGTCCAAAAGAGCGTGCAGAGATTTCAATGCTTGAAGGAGCGGTTTTG
GATATTAGATACGGTGTTTCGAGAATTGCATATAGTAAAGACTTTGAAAC
TCTCAAAGTTGATTTTCTTAGCAAGCTACCTGAAATGCTGAAAATGTTCG
AAGATCGTTTATGTCATAAAACATATTTAAATGGTGATCATGTAACCCAT
CCTGACTTCATGTTGTATGACGCTCTTGATGTTGTTTTATACATGGACCC
AATGTGCCTGGATGCGTTCCCAAAATTAGTTTGTTTTAAAAAACGTATTG
AAGCTATCCCACAAATTGATAAGTACTTGAAATCCAGCAAGTATATAGCA
TGGCCTTTGCAGGGCTGGCAAGCCACGTTTGGTGGTGGCGACCATCCTCC
AAAATCGGATCTGGAAGTTCTGTTCCAGGGGCCCCTGGGATCCTCATCTC
CCAGTCCGGGCAAGAGGCGGATGGACACGGACGTGGTCAAGCTCATCGAG
AGTAAACATGAGGTTACGATCCTGGGAGGACTTAATGAATTTGTAGTGAA
GTTTTATGGACCACAAGGAACACCATATGAAGGCGGAGTATGGAAAGTTA
GAGTGGACCTACCTGATAAATACCCTTTCAAATCTCCATCTATAGGATTC
ATGAATAAAATTTTCCATCCCAACATTGATGAAGCGTCAGGAACTGTGTG
TCTAGATGTAATTAATCAAACTTGGACAGCTCTCTATGATCTTACCAATA
TATTTGAGTCCTTCCTGCCTCAGTTATTGGCCTATCCTAACCCCATAGAT
CCTCTCAATGGTGACGCTGCAGCCATGTACCTCCACCGACCAGAAGAATA
CAAGCAGAAAATTAAAGAGTACATCCAGAAATACGCCACGGAGGAGGCGC
TGAAAGAACAGGAAGAGGGTACCGGGGACAGCTCATCGGAGAGCTCTATG
TCTGACTTTTCCGAAGATGAGGCCCAGGATATGGAGTTGTAGGCGGCCGC

Amino Acid Sequence

MSPILGYWKIKGLVQPTRLLLEYLEEKYEEHLYERDEGDKWRNKKFELGL
EFPNLPYYIDGDVKLTQSMAIIRYIADKHNMLGGCPKERAEISMLEGAVL
DIRYGVSRIAYSKDFETLKVDFLSKLPEMLKMFEDRLCHKTYLNGDHVTH
PDFMLYDALDVVLYMDPMCLDAFPKLVCFKKRIEAIPQIDKYLKSSKYIA
WPLQGWQATFGGGDHPPKSDLEVLFQGPLGSSSPSPGKRRMDTDVVKLIE
SKHEVTILGGLNEFVVKFYGPQGTPYEGGVWKVRVDLPDKYPFKSPSIGF
MNKIFHPNIDEASGTVCLDVINQTWTALYDLTNIFESFLPQLLAYPNPID
PLNGDAAAMYLHRPEEYKQKIKEYIQKYATEEALKEQEEGTGDSSSESSM
SDFSEDEAQDMEL*

• Vector Type: Bacterial
• Antibiotic: Amp
• DU Number: DU4161
• Unit Source: Protein Phosphorylation and Ubiquitylation Unit
• Location: PPU
• MTA: Dundee
• Notes: Bacterial expression with N terminal GST.