cDNA Clone CREB

• Plasmid: pCMV5-HA CREB1 S133E
• Parent Plasmid: pCMV5
• Expressed: HA-CREB1 S133E
• Genbank: NM_134442.2
• Tag: HA
• Species: Human

Sequence of Insert

GAATTCGCCACCATGTACCCATACGATGTGCCAGATTACGCCACCATGGA
ATCTGGAGCCGAGAACCAGCAGAGTGGAGATGCAGCTGTAACAGAAGCTG
AAAACCAACAAATGACAGTTCAAGCCCAGCCACAGATTGCCACATTAGCC
CAGGTATCTATGCCAGCAGCTCATGCAACATCATCTGCTCCCACCGTAAC
TCTAGTACAGCTGCCCAATGGGCAGACAGTTCAAGTCCATGGAGTCATTC
AGGCGGCCCAGCCATCAGTTATTCAGTCTCCACAAGTCCAAACAGTTCAG
TCTTCCTGTAAGGACTTAAAAAGACTTTTCTCCGGAACACAGATTTCAAC
TATTGCAGAAAGTGAAGATTCACAGGAGTCAGTGGATAGTGTAACTGATT
CCCAAAAGCGAAGGGAAATTCTTTCAAGGAGGCCTGAGTACAGGAAAATT
TTGAATGACTTATCTTCTGATGCACCAGGAGTGCCAAGGATTGAAGAAGA
GAAGTCTGAAGAGGAGACTTCAGCACCTGCCATCACCACTGTAACGGTGC
CAACTCCAATTTACCAAACTAGCAGTGGACAGTATATTGCCATTACCCAG
GGAGGAGCAATACAGCTGGCTAACAATGGTACCGATGGGGTACAGGGCCT
GCAAACATTAACCATGACCAATGCAGCAGCCACTCAGCCGGGTACTACCA
TTCTACAGTATGCACAGACCACTGATGGACAGCAGATCTTAGTGCCCAGC
AACCAAGTTGTTGTTCAAGCTGCCTCTGGAGACGTACAAACATACCAGAT
TCGCACAGCACCCACTAGCACTATTGCCCCTGGAGTTGTTATGGCATCCT
CCCCAGCACTTCCTACACAGCCTGCTGAAGAAGCAGCACGAAAGAGAGAG
GTCCGTCTAATGAAGAACAGGGAAGCAGCTCGAGAGTGTCGTAGAAAGAA
GAAAGAATATGTGAAATGTTTAGAAAACAGAGTGGCAGTGCTTGAAAATC
AAAACAAGACATTGATTGAGGAGCTAAAAGCACTTAAGGACCTTTACTGC
CACAAATCAGATTAAGGATCC

Amino Acid Sequence

MYPYDVPDYATMESGAENQQSGDAAVTEAENQQMTVQAQPQIATLAQVSM
PAAHATSSAPTVTLVQLPNGQTVQVHGVIQAAQPSVIQSPQVQTVQSSCK
DLKRLFSGTQISTIAESEDSQESVDSVTDSQKRREILSRRPEYRKILNDL
SSDAPGVPRIEEEKSEEETSAPAITTVTVPTPIYQTSSGQYIAITQGGAI
QLANNGTDGVQGLQTLTMTNAAATQPGTTILQYAQTTDGQQILVPSNQVV
VQAASGDVQTYQIRTAPTSTIAPGVVMASSPALPTQPAEEAARKREVRLM
KNREAARECRRKKKEYVKCLENRVAVLENQNKTLIEELKALKDLYCHKSD
*

• Vector Type: Mammalian
• Antibiotic: Amp
• DU Number: DU4108
• Unit Source: Protein Phosphorylation and Ubiquitylation Unit
• Location: PPU
• MTA: Dundee
• Notes: Mammalian expression with HA on the N terminus