cDNA Clone Cortactin

• Plasmid: pCMV5-HA Cortactin
• Parent Plasmid: pCMV5
• Expressed: HA-Cortactin
• Genbank: NP_612632
• Tag: HA
• Species: Human

Sequence of Insert

GAATTCGCCACCATGTACCCATACGATGTGCCAGATTACGCCTGGAAAGC
TTCAGCAGGCCACGCTGTGTCCATCGCCCAGGATGACGCGGGGGCCGATG
ACTGGGAGACCGACCCTGATTTTGTGAATGATGTGAGTGAGAAGGAGCAA
AGATGGGGTGCCAAGACGGTGCAGGGCTCCGGGCACCAGGAGCATATCAA
CATACACAAGCTGAGGGAGAATGTCTTTCAAGAGCATCAGACCCTTAAGG
AGAAGGAACTTGAAACAGGACCAAAAGCTTCCCATGGCTATGGAGGGAAA
TTTGGTGTGGAACAAGACCGAATGGATAAGTCAGCTGTCGGCCACGAATA
TCAGTCGAAACTTTCCAAGCACTGCTCGCAGGTGGACTCGGTCCGTGGCT
TCGGAGGCAAGTTTGGTGTCCAGATGGACAGAGTTGATCAGTCTGCTGTA
GGCTTTGAATACCAGGGGAAGACTGAGAAGCATGCCTCCCAGAAAGACTA
CTCCAGTGGTTTTGGCGGCAAGTATGGCGTGCAGGCCGACCGAGTAGACA
AGAGCGCGGTGGGCTTCGACTACCAGGGCAAGACGGAGAAGCACGAGTCA
CAGAGAGATTACTCCAAAGGTTTCGGCGGCAAATACGGTATCGACAAGGA
CAAAGTGGATAAGAGCGCCGTTGGCTTTGAGTATCAAGGCAAAACGGAGA
AGCACGAGTCCCAGAAAGACTATGTGAAAGGGTTTGGAGGAAAATTTGGT
GTGCAGACAGACAGACAAGACAAATGTGCCCTTGGCTGGGATCACCAGGA
GAAATTGCAGCTGCATGAATCCCAAAAAGACTACTCCAAAGGATTCGGCG
GGAAGTATGGGGTGCAGAAGGATCGGATGGATAAGAATGCGTCAACCTTT
GAGGATGTCACCCAGGTGTCCTCTGCCTACCAGAAGACAGTACCTGTCGA
AGCTGTGACCAGCAAAACAAGTAACATCAGAGCTAACTTTGAAAACCTCG
CTAAGGAGAAAGAGCAGGAGGACAGGCGGAAGGCGGAGGCGGAGAGAGCC
CAGCGGATGGCCAAGGAGCGGCAGGAGCAGGAAGAGGCCAGGAGGAAGCT
GGAGGAGCAAGCCAGAGCCAAAACGCAAACGCCCCCTGTGTCGCCCGCAC
CTCAGCCAACCGAGGAGAGGCTGCCCTCGAGCCCCGTCTATGAGGATGCG
GCTTCCTTCAAGGCAGAGCTGAGCTACAGAGGCCCTGTGAGTGGGACGGA
GCCGGAGCCCGTGTACAGCATGGAGGCCGCTGACTACCGAGAGGCCAGCA
GCCAGCAGGGCCTGGCCTATGCCACAGAGGCTGTCTATGAAAGCGCAGAG
GCCCCGGGCCACTATCCCGCAGAGGACAGCACCTATGATGAGTACGAGAA
CGATCTGGGGATCACAGCCGTCGCCCTGTACGACTACCAGGCTGCGGGCG
ATGATGAGATCTCATTTGACCCTGATGACATCATCACCAACATCGAGATG
ATTGACGACGGCTGGTGGCGCGGGGTGTGCAAGGGCCGGTACGGGCTCTT
CCCAGCCAACTATGTGGAGCTGCGGCAGTAGGGATAAGGGCGAATTC

Amino Acid Sequence

MYPYDVPDYAWKASAGHAVSIAQDDAGADDWETDPDFVNDVSEKEQRWGA
KTVQGSGHQEHINIHKLRENVFQEHQTLKEKELETGPKASHGYGGKFGVE
QDRMDKSAVGHEYQSKLSKHCSQVDSVRGFGGKFGVQMDRVDQSAVGFEY
QGKTEKHASQKDYSSGFGGKYGVQADRVDKSAVGFDYQGKTEKHESQRDY
SKGFGGKYGIDKDKVDKSAVGFEYQGKTEKHESQKDYVKGFGGKFGVQTD
RQDKCALGWDHQEKLQLHESQKDYSKGFGGKYGVQKDRMDKNASTFEDVT
QVSSAYQKTVPVEAVTSKTSNIRANFENLAKEKEQEDRRKAEAERAQRMA
KERQEQEEARRKLEEQARAKTQTPPVSPAPQPTEERLPSSPVYEDAASFK
AELSYRGPVSGTEPEPVYSMEAADYREASSQQGLAYATEAVYESAEAPGH
YPAEDSTYDEYENDLGITAVALYDYQAAGDDEISFDPDDIITNIEMIDDG
WWRGVCKGRYGLFPANYVELRQ*

• Vector Type: Mammalian
• Antibiotic: Amp
• DU Number: DU4067
• Unit Source: Protein Phosphorylation and Ubiquitylation Unit
• Location: PPU
• MTA: Dundee
• Notes: Mammalian expression with N terminal HA tag.