cDNA Clone Speg (mouse)

• Plasmid: pcDNA5-FRT/TO-GFP Speg (mouse) (protein ID ENSMUSP00000045263)
• Parent Plasmid: pcDNA5 FRT/TO GFP
• Expressed: GFP-Speg (mouse)
• Genbank: Ensembl protein ID ENSMUSP00000045263, transcript ID ENSMUST00000038230*
• Tag: GFP
• Species: Mouse

Sequence of Insert

AGATCTATGAAGAAGCTGTGGGTGAAGAAGCGTTTCCAGAAAACCGGCCA
TTCCCGCCGGGCCTTTGGCCGACTTACCCATGACTCGGAGACTGCCGAGG
ATGACATCAGCGATGTGCCGGGGACCCAGCGCCTGGAGCTTCGGGATGAC
AGGGCCTTCAGCACCCCCACGGGGGGCTCTGATACCCTGGTGGGTACCTC
CCTGGACACACCCCCGACCTCCGTGACAGGCACCTCAGAGGAGCAAGTGA
GCTGGTGGGGCAGCGGGCAGACGGTCCTGGAGCAGGAAGCGGGCAGCGGG
GGTGGCACCCGCCCCCTCCCAGGCAGCCCAAGGCAAGCACAGACAACCGG
GGCCGGGCCACGGCACCTGGGGGTGGAGCCGCTGGTGCGGGCATCGCGAG
CTAATCTGGTGGGCGCAAGCTGGGGGTCAGAGGATAGCCTTTCGGTGGCC
AGTGACCTCTATGGCAGCGCGTTCAGTCTGTACAGAGGACGGGCGCTCTC
GATCCACGTCAGCATTCCTCCGAGTGGATTGCACAGAGAGGAGCCTGACC
TTCAGCCTCAGCCAGCCAGTGATGCCCTTCGTCCACGCCCTGCCCTTCCA
CCTCCCTCCAAATCCGCTTTGCTTCCCCCACCATCTCCTCGGGTGGGAAA
GAGGGCTCTGCCAGGACCTAGTACCCAGCCCCCAGCTACTCCCACTTCGC
CCCATCGGCGTGCTCAGGAGCCCTCGCTGCCTGAGGACATCACCACCACG
GAAGAGAAGCGAGGGAAAAAGCCCAAGTCATCCGGGCCGTCGCTGGCGGG
CACAGTGGAGTCCCGGCCCCAGACGCCACTGAGCGAGGCTTCGGGTCGCC
TGTCAGCATTGGGCCGCTCGCCCCGGCTGGTGCGCGCGGGGTCCCGCATC
CTGGACAAGCTACAGTTCTTCGAAGAGCGGCGACGCAGCCTGGAGCGCAG
CGACTCGCCGCCAGCGCCCCTGCGGCCCTGGGTGCCCCTGCGCAAGGCTC
GCTCGCTGGAGCAGCCGAAGTCCGAGGGCGGTGCGGCGTGGGGCACACCC
GAGGCCTCGCAGGAGGAGCTGCGGTCACCTCGGGGCAGTGTGGCAGAGCG
GCGTCGCCTGTTCCAGCAAAAGGCGGCCTCGTTGGATGAACGCACGCGAC
AACGCAGTGCAACCTCGGACCTCGAACTCCGCTTCGCCCAGGAGCTGGGT
CGCATCCGCCGATCTACGTCGCGGGAGGAGCTGGTGCGTTCGCACGAGTC
CCTGCGTGCCACGCTGCAGCGCGCCCCATCCCCTCGGGAGCCCGGCGAGC
CCCCACTCTTCTCCCGGCCTTCCACACCCAAGACCTCACGGGCTGTGAGC
CCGGCTGCCACCCAGCCGCCGCCTCCTAGTGGTGCGGGCAAATCTGGGGA
CGAGCCTGGGAGGCCCCGAAGCAGAGGGCCGGTGGGCAGGACTGAACCGG
GGGAAGGCCCGCAGCAGGAGATCAAGCGTCGGGACCAATTCCCGCTAACC
AGGAGCAGAGCCATCCAGGAGTGCAGGAGCCCTGTGCCGCCCTACACCGC
GGATCCCCCGGAGAGCAGGACAAAAGCCCCCTCCGGTCGCAAGCGGGAAC
CCCCTGCTCAAGCGGTGCGCTTTCTGCCCTGGGCCACTCCGGGAGTGGAG
GACTCTGTTCTGCCCCAAACCTTGGAGAAGAATAGAGCGGGACCCGAGGC
TGAGAAGAGGCTTCGCAGAGGACCTGAGGAGGATGGCCCCTGGGGGCCCT
GGGACCGCAGAGGGACCCGCAGCCAAGGCAAAGGTCGCCGTGCTCGGCCT
ACTTCCCCCGAGCTCGAGTCCTCAGACGACTCCTATGTGTCCGCTGGGGA
AGAGCCCCTGGAGGCACCCGTGTTTGAGATCCCTCTGCAGAATATGGTGG
TGGCGCCAGGAGCTGACGTGCTACTTAAGTGTATCATCACCGCCAACCCC
CCACCCCAAGTGTCCTGGAAAAAGGATGGGTCCATGTTGCACAGCGAGGG
TCGTCTTCTCATCCGGGCTGAAGGTGAACGGCACACACTGCTGCTCAGAG
AGGCCCAGGCTGCCGATGCTGGGAGCTACACAGCCACTGCCACCAACGAA
CTGGGCCAAGCTACCTGTGCTTCTTCACTGGCTGTGAGACCTGGCGGCTC
CACATCCCCTTTCAGCAGCCCCATCACCTCTGATGAGGAGTACCTGAGCC
CCCCAGAGGAGTTCCCAGAGCCTGGGGAGACCTGGCCCCGAACCCCTACC
ATGAAGCTCAGTCCCAGCCAGGATCATGATTCCTCCGACTCTTCTTCCAA
GGCACCCCCAACGTTCAAGGTCTCACTCATGGACCAATCGGTGAGAGAAG
GTCAAGATGTCATTATGAGCATCCGTGTGCAGGGAGAGCCCAAGCCTGTG
GTTTCCTGGCTGAGGAATCGACAGCCCGTGCGCCCAGACCAGCGGCGCTT
TGCAGAGGAGGCCGAGGGTGGGCTCTGCCGCTTGAGGATCCTGGCTGCTG
AACGGGGCGATGCTGGTTTCTACACATGCAAGGCGGTCAACGAATATGGC
GCTCGGCAGTGCGAGGCCCGCCTGGAGGTCCGAGCACACCCTGAAAGCCG
GTCCCTGGCCGTGCTGGCCCCCCTGCAGGACGTGGACGTGGGGGCCGGGG
AGATGGCGCTGTTTGAGTGCCTGGTGGCAGGTCCAGCTGACGTCGAGGTG
GACTGGCTGTGCCGGGGCCGCCTGCTGCAGCCTGCGCTGCTCAAATGCAA
GATGCATTTTGATGGCCGCAAGTGCAAGCTGCTGCTCACCTCTGTGCACG
AGGACGACAGTGGTGTCTACACCTGCAAGCTGAGCACAGCCAAAGATGAA
CTGACCTGCAGTGCCCGGCTGACGGTTCGGCCGTCGCTGGCGCCTCTCTT
CACCCGGTTGCTGGAAGATGTGGAGGTGTTGGAAGGCCGTGCTGCCCGCT
TGGATTGCAAGATCAGCGGTACCCCGCCTCCCTCCGTTACCTGGACTCAT
TTTGGCCACCCCGTGAATGAGGGTGACAACTTGCGGCTTCAACAGGATGG
AGGTCTACACTCACTGCACATTGCCCGGGTGGGCAGTGAGGACGAAGGAC
TCTATGAAGTCAGTGCCACCAACACTCATGGCCAGGCCCACTGCTCCGCC
CAGCTGTATGTGGAGGAGCCTCGGACAGCTGCCTCTGGCCCCAGCTCGAA
GCTGGAGAAGATGCCATCTATCCCTGAGGAGCCGGAGCACGGAGACCTAG
AGAGGCTGTCTATTCCTGACTTCCTACGGCCACTGCAAGACCTGGAAGTG
GGACTAGCTAAGGAGGCCATGCTGGAGTGCCAAGTGACCGGCCTGCCCTA
TCCCACCATCAGCTGGTTCCACAATGGCCACCGCATTCAAAGCAGTGACG
ACCGACGCATGACACAGTACAGGGACATACATCGCCTGGTGTTCCCAGCT
GTGGGGCCTCAGCATGCTGGTGTCTATAAGAGTGTAATCGCCAACAAGCT
GGGCAAAGCGGCCTGCTACGCCCATCTCTATGTCACAGATGTGGTTCCAG
GCCCTCCAGATGGTGCTCCGGAAGTAGTAGCTGTGACCGGAAGGATGGTC
ACACTATCGTGGAACCCACCCAGGAGTCTGGACATGGCTATTGACCCAGA
TTCCCTAACATACACTGTGCAGCACCAGGTGTTGGGCTCGGACCAGTGGA
CGGCACTGGTCACAGGCCTGCGGGAGCCTGCGTGGGCAGCCACAGGGCTG
AAGAAGGGGATCCAGCACATCTTCCGGGTCCTCAGCTCCAGTGGCAAGAG
CAGCAGCAAGCCCTCAGCACCGTCGGAACCCGTGCAGCTGCTAGAGCATG
GCCCACCCCTGGAAGAGGCGCCTGCTGTGCTGGACAAGCAGGACATTGTG
TATGTGGTAGAGGGCCAGCCTGCCTGTGTCACCGTCACTTTTAACCATGT
GGAGGCCCAGGTTGTCTGGAGGAGCTGCCGGGGAGCCCTCCTCGAGGCGC
GCACAGGTGTGTATGAACTGAGTCAGCCAGATGACGACCAGTATTGTCTG
CGGATCTGCCGGGTGAGCCGACGAGACCTGGGACCCCTTACTTGCAGTGC
CCGAAACCGCCACGGCACAAAGGCCTGCTCGGTCACCCTGGAGCTGGCAG
AGGCACCTCGGTTTGAGTCTATCATGGAAGATGTGGAGGTGGGGCCTGGA
GAAACCGCTCGCTTTGCTGTGGTAGTTGAGGGGAAACCACTGCCAGACAT
CATGTGGTACAAGGATGAGGTGCTGCTGGCCGAGAGCAACCATGTGAGCT
TCGTGTATGAGGAGAACGAGTGTTCCCTAGTGCTGCTCAGCGCAGGAAGC
CAGGATGGAGGCGTCTATACCTGTACCGCCCGGAACCTGGCAGGCGAAGT
CTCCTGCAAGGCGGAGCTGTCTGTGCTTTCAGCTCAGACAGCCATGGAGG
TCGAGGGGGTCGGAGAGGATGAGGAACACCGAGGAAGAAGACTTAGCGAC
TACTACGACATCCACCAAGAGATTGGCAGGGGTGCCTTCTCCTACCTGCG
GCGTGTAGTGGAGAGGAGCTCTGGCCTGGAGTTTGCAGCCAAGTTCATCC
CTAGCCAGGCTAAGCCTAAAGCATCAGCAAGACGCGAGGCGCGCTTGCTG
GCCCGGCTCCAGCATGGCTGTGTGCTCTACTTCCACGAAGCTTTTGAGAG
GCGCAGGGGACTTGTCATAGTCACGGAGCTCTGCACAGAAGAGCTTCTAG
AGCGCATGGCCAGGAAGCCCACCGTGTGTGAGTCTGAGACTCGGACCTAT
ATGCGTCAAGTGCTAGAGGGGATATGCTATCTGCATCAGAGCCATGTGCT
GCACCTGGATGTCAAGCCGGAGAACCTGCTTGTGTGGGATGGTGCAGGGG
GTGAAGAGCAGGTGCGCATCTGTGACTTCGGGAACGCCCAGGAACTCACT
CCGGGAGAGCCCCAGTACTGTCAGTATGGCACGCCAGAGTTTGTAGCACC
TGAGATTGTCAACCAGAGCCCTGTGTCTGGAGTCACTGACATCTGGCCCG
TGGGCGTCGTTGCCTTTCTCTGTTTGACGGGCATCTCCCCATTTGTTGGG
GAGAATGACCGGACAACACTCATGAATATCCGAAACTACAACGTGGCATT
TGAGGAGACTACGTTCCTGAGTCTGAGCAGGGAGGCCCGGGGCTTCCTCA
TCAAAGTGCTGGTGCAGGATCGACTGAGACCTACTGCAGAAGAGACATTG
GAACATCCTTGGTTCAAAACAGAAGCAAAGGGTGCAGAGGTGAGCACAGA
TCACCTGAAGCTCTTCCTGTCACGGAGGAGGTGGCAGCGCTCTCAAATCA
GCTACAAGTGCCACCTGGTGCTGCGCCCCATTCCGGAACTGCTACGGGCT
CCCCCGGAGCGGGTGTGGGTGGCCATGCCTAGAAGACAACCCCCGAGTGG
GGGACTCTCATCCTCTTCAGACTCAGAAGAGGAAGAGTTGGAGGAACTTC
CCTCAGTACCCCGCCCCCTGCAGCCTGAGTTCTCAGGTTCTCGGGTATCC
CTCACCGACATCCCCACCGAAGATGAGGCTCTGGGGACCCCGGAAGCTGG
GGCTGCCACCCCCATGGACTGGCAAGAGCAGGAAAGGACTCCCTCTAAGG
ACCAGGAGGCCCCCAGCCCTGAGGCACTCCCCTCTCCAGGCCAGGAGTCC
CCTGATGGGCCTAGCCCCAGGCGGCCAGAGCTCCGCAGGGGTAGCTCTGC
TGAGAGTGCCCTGCCCCGAGTCGGGTCGCGGGAACCGGGCCGGAGCTTGC
ACAAGGCAGCATCCGTGGAGCTGCCGCAGCGCAGGAGCCCCAGCCCGGGT
GCCACCCGCCTGACTCGGGGAGGCCTGGGTGAGGGCGAGTATGCCCAGAG
GCTGCAGGCCCTGCGTCAGCGGCTGCTGAGGGGAGGCCCCGAGGATGGCA
AGGTCAGCGGCCTCAGGGGACCCCTGCTGGAGAGCCTGGGGGGCCGTGCA
CGGGATCCCAGGATGGCAAGGGCTGCCTCCAGCGAGGCTGCCCCCCACCA
CCAGCCCCCTCCGGAGTCCCGGGGTCTGCAAAAGAGCAGCAGCTTCTCTC
AGGGCGAGGCAGAGCCCCGGGGTCGGCACCGCCGCGCGGGGGCACCCCTC
GAAATCCCCGTGGCCAGACTTGGGGCTCGCAGGCTGCAGGAGTCTCCCTC
CTTATCTGCCCTTAGCGAGACCCAGCCACCGAGCCCTGCGAGGCCCAGCG
TCCCCAAACTCAGTATCACCAAGTCCCCGGAACCTTCCGCTGTCACATCC
AGAGACTCTCCCCAGCCCCCGGAACCTCAGCCTGTCCCAGAAAAGGTCCC
TGAGCCCAAACCAGAACCAGTCCGAGCTGCAAAACCAGCACAACCCCCTT
TGGCTCTGCAAATGCCAACGCAGCCTCTCACCCCCTATGCCCAGATCATG
CAGTCGCTCCAGCTGTCCAGTCCCACCCTGTCCCCACAGGACCCCGCTGT
GCCTCCATCAGAGCCCAAGCCCCACGCGGCTGTGTTTGCCAGGGTGGCTT
CCCCACCACCCGGAGTCTCTGAGAAGCGCGTGCCTTCGGCCAGAACTCCC
CCAGTGCTAGCCGAGAAAGCCCGAGTCCCCACGGTGCCCCCCAGGCCAGG
CAGCAGCCTCAGCGGCAGCATCGAAAACCTTGAATCAGAGGCTGTGTTCG
AGGCTAAGTTCAAACGCAGCCGCGAATCGCCACTGTCGCGGGGCCTGAGG
CTACTGAGCCGCTCGCGCTCGGAGGAGCGCGGTCCCTTCCGTGGGGCGGA
GGACGACGGCATATACCGGCCCAGTCCGGCAGGAACGCCGCTAGAGCTGG
TGCGACGGCCCGAGCGCTCGCGCTCCGTGCAGGACCTCAGGGTAGCCGGA
GAGCCGGGCCTTGTGCGTCGCCTTTCGCTGTCCCTGTCGCAGAAGCTGCG
GCGGACACCGCCGGGACAGCGACACCCTGCCTGGGAGTCCCGCAGCGGAG
ACGGGGAGAGCTCCGAGGGCGGCAGCTCAGCTCGGGCATCCCCAGTGCTG
GCGGTACGCAGGAGACTCAGCTCCACGCTGGAGCGTCTGTCCAGCCGTTT
GCAGCGCAGCGGCAGCAGCGAGGACTCCGGGGGCGCCTCGGGTCGCAGCA
CGCCGCTGTTTGGACGGCTGCGCAGGGCTACTTCGGAAGGGGAGAGCCTG
CGACGCCTCGGTGTTCCGCACAATCAGCTGGGCTCTCAGACTGGTGCCAC
TACACCTTCCGCTGAGTCTCTGGGCTCAGAGGCCAGTGGCACATCGGGTT
CTTCAGCTCCTGGAGAAAGCCGAAGCCGGCACCGGTGGGGCCTTTCCAGG
CTGCGCAAGGACAAGGGATTATCTCAGCCAAACCTCTCCTCCAGTGTCCA
AGAGGACTTGGGTCACCAGTATGTGCCCAGTGAGTCAGATTTCCCCCCAG
TCTTCCACATCAAACTCAAGGACCAGGTGCTGTTGGAGGGGGAAGCAGCC
ACCCTGCTCTGCCTACCCGCAGCCTGCCCAGCACCCCGCATCTCCTGGAT
GAAAGATAAGCAGTCCCTGCGTTCAGAGCCCTCAGTGGTCATTGTGTCCT
GCAAAGATGGACGGCAGCTGCTGAGCATACCCCGGGCCGGCAAGCGGCAT
GCTGGGCTCTATGAGTGCTCAGCCACCAATGTCCTGGGCAGCATTACCAG
CTCCTGTACTGTGGCCGTGGCCCGCATTCCAGGGAAGCTAGCTCCTCCAG
AGGTGCCCCAGACCTACCATGACACAGCACTAGTAGTGTGGAAGCCAGGA
GACGGTCGGGCACCTTGCACATACACTCTGGAGCGGCGGGTGGATGGGGA
ATCTGTCTGGCACCCTGTGAGCTCAGGCATCCCCGACTGTTATTACAATG
TGACTCAGCTGCCTGTCGGCGTGACCGTGCGGTTCCGTGTGGCCTGTTCC
AATCGTGCTGGGCAGGGGCCCTTCAGCAACCCTTCTGAGAAGGTCTTCAT
CCGAGGCACTCCAGATTCTCCTGCTCAGCCAGCTGCTGCTCCCCGAGATG
CCCCTGTTACCTCAGGGCCAACCAGGGCCCCACCGCCTGACTCTCCTACC
TCACTGGCCCCCACCCCCGCTCTTGCGCCCCCAGCCTCCCAGGCATCCAC
TCTCAGCCCCTCAACTTCCTCCATGTCTGCCAACCAGGCCTTGTCCTCAC
TCAAGGCTGTGGGTCCACCACCCGCAACCCCTCCACGAAAGCACAGGGGC
CTTCTGGCCACCCAGCAAGCAGAGCCATCCCCACCCAGTATCGTGGTCAC
CCCAAGTGAACCCAGGTCTTTTGTCCCTGACACTGGAACCCTGACCCCAA
CCTCTAGTCCTCAAGGGGTTAAACCAGCACCTTCCTCAACTTCCTTGTAT
ATGGTGACTTCCTTCGTGTCTGCACCTCCAGCCCCCCAGGCCCCAGCCCC
TGAGCCCCCTCCTGAGCCCACCAAGGTGACTGTGCGGAGCCTCAGCCCAG
CCAAGGAGGTGGTCAGCTCCCCAACTCCTGAGAGCACCACTCTTCGGCAG
GGTCCCCCTCAGAAACCCTACACCTTCCTGGAGGAGAAGGCCAGGGGCCG
CTTTGGCGTTGTGCGGTCATGCCGGGAGAATGCTACGGGCCGAACGTTTG
TCGCCAAGATCGTGCCGTACGCCGCGGAGGGCAAGCGACGCGTGCTGCAG
GAATACGAGGTGCTTCGGACGCTGCACCACGAGCGGCTCATGTCCCTGCA
CGAGGCCTACATCACCCCTCGGTACCTCGTGCTTATCGCTGAGAGCTGTG
GCAACCGGGAACTCCTCTGTGGGCTCAGCGACAGATTCCGGTATTCAGAA
GATGACGTGGCCACCTATGTGGTGCAGCTGCTACAAGGCCTGGACTACCT
CCACGGCCACCACGTGCTACACCTGGACATCAAGCCGGACAATCTACTGT
TGGCCGCTGACAATGCCCTTAAAATTGTGGACTTCGGCAGTGCTCAGCCC
TATAACCCTCAGGCCCTGAAGCCACTTGGCCACCGCACAGGCACTCTGGA
GTTCATGGCTCCTGAGATGGTGAAGGGAGACCCCATTGGCTCTGCCACGG
ACATCTGGGGAGCGGGTGTGCTCACCTACATCATGCTTAGTGGGTACTCC
CCATTCTATGAGCCAGACCCCCAGGAAACAGAGGCTCGGATTGTTGGGGG
TCGCTTTGATGCCTTCCAGTTGTATCCTAACACATCCCAGAGTGCCACCC
TCTTCTTGAGAAAGGTCCTCTCAGTACATCCCTGGAGCCGGCCCTCTCTG
CAGGACTGCTTGGCCCACCCATGGCTGCAAGATGCCTACCTGATGAAGCT
GCGCCGCCAGACACTCACCTTCACCACCAACCGGCTCAAGGAATTCCTGG
GCGAGCAGCGGCGACGTCGGGCTGAGGCTGCTACCCGTCACAAGGTGCTG
CTCCGCTCCTACCCTGGCAGCCCCTAGCGGCCGC

Amino Acid Sequence

MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTLKFICT
TGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIF
FKDDGNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHN
VYIMADKQKNGIKVNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNH
YLSTQSALSKDPNEKRDHMVLLEFVTAAGITLGMDELYKSGLGSMKKLWV
KKRFQKTGHSRRAFGRLTHDSETAEDDISDVPGTQRLELRDDRAFSTPTG
GSDTLVGTSLDTPPTSVTGTSEEQVSWWGSGQTVLEQEAGSGGGTRPLPG
SPRQAQTTGAGPRHLGVEPLVRASRANLVGASWGSEDSLSVASDLYGSAF
SLYRGRALSIHVSIPPSGLHREEPDLQPQPASDALRPRPALPPPSKSALL
PPPSPRVGKRALPGPSTQPPATPTSPHRRAQEPSLPEDITTTEEKRGKKP
KSSGPSLAGTVESRPQTPLSEASGRLSALGRSPRLVRAGSRILDKLQFFE
ERRRSLERSDSPPAPLRPWVPLRKARSLEQPKSEGGAAWGTPEASQEELR
SPRGSVAERRRLFQQKAASLDERTRQRSATSDLELRFAQELGRIRRSTSR
EELVRSHESLRATLQRAPSPREPGEPPLFSRPSTPKTSRAVSPAATQPPP
PSGAGKSGDEPGRPRSRGPVGRTEPGEGPQQEIKRRDQFPLTRSRAIQEC
RSPVPPYTADPPESRTKAPSGRKREPPAQAVRFLPWATPGVEDSVLPQTL
EKNRAGPEAEKRLRRGPEEDGPWGPWDRRGTRSQGKGRRARPTSPELESS
DDSYVSAGEEPLEAPVFEIPLQNMVVAPGADVLLKCIITANPPPQVSWKK
DGSMLHSEGRLLIRAEGERHTLLLREAQAADAGSYTATATNELGQATCAS
SLAVRPGGSTSPFSSPITSDEEYLSPPEEFPEPGETWPRTPTMKLSPSQD
HDSSDSSSKAPPTFKVSLMDQSVREGQDVIMSIRVQGEPKPVVSWLRNRQ
PVRPDQRRFAEEAEGGLCRLRILAAERGDAGFYTCKAVNEYGARQCEARL
EVRAHPESRSLAVLAPLQDVDVGAGEMALFECLVAGPADVEVDWLCRGRL
LQPALLKCKMHFDGRKCKLLLTSVHEDDSGVYTCKLSTAKDELTCSARLT
VRPSLAPLFTRLLEDVEVLEGRAARLDCKISGTPPPSVTWTHFGHPVNEG
DNLRLQQDGGLHSLHIARVGSEDEGLYEVSATNTHGQAHCSAQLYVEEPR
TAASGPSSKLEKMPSIPEEPEHGDLERLSIPDFLRPLQDLEVGLAKEAML
ECQVTGLPYPTISWFHNGHRIQSSDDRRMTQYRDIHRLVFPAVGPQHAGV
YKSVIANKLGKAACYAHLYVTDVVPGPPDGAPEVVAVTGRMVTLSWNPPR
SLDMAIDPDSLTYTVQHQVLGSDQWTALVTGLREPAWAATGLKKGIQHIF
RVLSSSGKSSSKPSAPSEPVQLLEHGPPLEEAPAVLDKQDIVYVVEGQPA
CVTVTFNHVEAQVVWRSCRGALLEARTGVYELSQPDDDQYCLRICRVSRR
DLGPLTCSARNRHGTKACSVTLELAEAPRFESIMEDVEVGPGETARFAVV
VEGKPLPDIMWYKDEVLLAESNHVSFVYEENECSLVLLSAGSQDGGVYTC
TARNLAGEVSCKAELSVLSAQTAMEVEGVGEDEEHRGRRLSDYYDIHQEI
GRGAFSYLRRVVERSSGLEFAAKFIPSQAKPKASARREARLLARLQHGCV
LYFHEAFERRRGLVIVTELCTEELLERMARKPTVCESETRTYMRQVLEGI
CYLHQSHVLHLDVKPENLLVWDGAGGEEQVRICDFGNAQELTPGEPQYCQ
YGTPEFVAPEIVNQSPVSGVTDIWPVGVVAFLCLTGISPFVGENDRTTLM
NIRNYNVAFEETTFLSLSREARGFLIKVLVQDRLRPTAEETLEHPWFKTE
AKGAEVSTDHLKLFLSRRRWQRSQISYKCHLVLRPIPELLRAPPERVWVA
MPRRQPPSGGLSSSSDSEEEELEELPSVPRPLQPEFSGSRVSLTDIPTED
EALGTPEAGAATPMDWQEQERTPSKDQEAPSPEALPSPGQESPDGPSPRR
PELRRGSSAESALPRVGSREPGRSLHKAASVELPQRRSPSPGATRLTRGG
LGEGEYAQRLQALRQRLLRGGPEDGKVSGLRGPLLESLGGRARDPRMARA
ASSEAAPHHQPPPESRGLQKSSSFSQGEAEPRGRHRRAGAPLEIPVARLG
ARRLQESPSLSALSETQPPSPARPSVPKLSITKSPEPSAVTSRDSPQPPE
PQPVPEKVPEPKPEPVRAAKPAQPPLALQMPTQPLTPYAQIMQSLQLSSP
TLSPQDPAVPPSEPKPHAAVFARVASPPPGVSEKRVPSARTPPVLAEKAR
VPTVPPRPGSSLSGSIENLESEAVFEAKFKRSRESPLSRGLRLLSRSRSE
ERGPFRGAEDDGIYRPSPAGTPLELVRRPERSRSVQDLRVAGEPGLVRRL
SLSLSQKLRRTPPGQRHPAWESRSGDGESSEGGSSARASPVLAVRRRLSS
TLERLSSRLQRSGSSEDSGGASGRSTPLFGRLRRATSEGESLRRLGVPHN
QLGSQTGATTPSAESLGSEASGTSGSSAPGESRSRHRWGLSRLRKDKGLS
QPNLSSSVQEDLGHQYVPSESDFPPVFHIKLKDQVLLEGEAATLLCLPAA
CPAPRISWMKDKQSLRSEPSVVIVSCKDGRQLLSIPRAGKRHAGLYECSA
TNVLGSITSSCTVAVARIPGKLAPPEVPQTYHDTALVVWKPGDGRAPCTY
TLERRVDGESVWHPVSSGIPDCYYNVTQLPVGVTVRFRVACSNRAGQGPF
SNPSEKVFIRGTPDSPAQPAAAPRDAPVTSGPTRAPPPDSPTSLAPTPAL
APPASQASTLSPSTSSMSANQALSSLKAVGPPPATPPRKHRGLLATQQAE
PSPPSIVVTPSEPRSFVPDTGTLTPTSSPQGVKPAPSSTSLYMVTSFVSA
PPAPQAPAPEPPPEPTKVTVRSLSPAKEVVSSPTPESTTLRQGPPQKPYT
FLEEKARGRFGVVRSCRENATGRTFVAKIVPYAAEGKRRVLQEYEVLRTL
HHERLMSLHEAYITPRYLVLIAESCGNRELLCGLSDRFRYSEDDVATYVV
QLLQGLDYLHGHHVLHLDIKPDNLLLAADNALKIVDFGSAQPYNPQALKP
LGHRTGTLEFMAPEMVKGDPIGSATDIWGAGVLTYIMLSGYSPFYEPDPQ
ETEARIVGGRFDAFQLYPNTSQSATLFLRKVLSVHPWSRPSLQDCLAHPW
LQDAYLMKLRRQTLTFTTNRLKEFLGEQRRRRAEAATRHKVLLRSYPGSP
*

• Vector Type: Mammalian
• Antibiotic: Amp
• DU Number: DU36474
• Unit Source: Protein Phosphorylation and Ubiquitylation Unit
• Location: PPU
• MTA: Dundee
• Notes: With silent mutations to kill two internal BglII sites, and introduce HindIII site for 3-way cloning.; CAUTION! Large plasmid – grow at low temperature.