cDNA Clone FAK (PTK2)

• Plasmid: pGEX6P-3-FAK (PTK2) isoform 1 delta V744-E789
• Parent Plasmid: pGEX6P3
• Expressed: GST-FAK isoform 1 delta V744-E789
• Genbank: NM_153831*
• Tag: GST
• Species: Human

Sequence of Insert

GGATCCATGGCAGCTGCTTACCTTGACCCCAACTTGAATCACACACCAAA
TTCGAGTACTAAGACTCACCTGGGTACTGGTATGGAACGTTCTCCTGGTG
CAATGGAGCGAGTATTAAAGGTCTTTCATTATTTTGAAAGCAATAGTGAG
CCAACCACCTGGGCCAGTATTATCAGGCATGGAGATGCTACTGATGTCAG
GGGCATCATTCAGAAGATAGTGGACAGTCACAAAGTAAAGCATGTGGCCT
GCTATGGATTCCGCCTCAGTCACCTGCGGTCAGAGGAGGTTCACTGGCTT
CACGTGGATATGGGCGTCTCCAGTGTGAGGGAGAAGTATGAGCTTGCTCA
CCCACCAGAGGAGTGGAAATATGAATTGAGAATTCGTTATTTGCCAAAAG
GATTTCTAAACCAGTTTACTGAAGATAAGCCAACTTTGAATTTCTTCTAT
CAACAGGTGAAGAGCGATTATATGTTAGAGATAGCTGATCAAGTGGACCA
GGAAATTGCTTTGAAGTTGGGTTGTCTAGAAATACGGCGATCATACTGGG
AGATGCGGGGCAATGCACTAGAAAAGAAGTCTAACTATGAAGTATTAGAA
AAAGATGTTGGTTTAAAGCGATTTTTTCCTAAGAGTTTACTGGATTCTGT
CAAGGCCAAAACACTAAGAAAACTGATCCAACAAACATTTAGACAATTTG
CCAACCTTAATAGAGAAGAAAGTATTCTGAAATTCTTTGAGATCCTGTCT
CCAGTCTACAGATTTGATAAGGAATGCTTCAAGTGTGCTCTTGGTTCAAG
CTGGATTATTTCAGTGGAACTGGCAATCGGCCCAGAAGAAGGAATCAGTT
ACCTAACGGACAAGGGCTGCAATCCCACACATCTTGCTGACTTCACTCAA
GTGCAAACCATTCAGTATTCAAACAGTGAAGACAAGGACAGAAAAGGAAT
GCTACAACTAAAAATAGCAGGTGCACCCGAGCCTCTGACAGTGACGGCAC
CATCCCTAACCATTGCGGAGAATATGGCTGACCTAATAGATGGGTACTGC
CGGCTGGTGAATGGAACCTCGCAGTCATTTATCATCAGACCTCAGAAAGA
AGGTGAACGGGCTTTGCCATCAATACCAAAGTTGGCCAACAGCGAAAAGC
AAGGCATGCGGACACACGCCGTCTCTGTGTCAGAAACAGATGATTATGCT
GAGATTATAGATGAAGAAGATACTTACACCATGCCCTCAACCAGGGATTA
TGAGATTCAAAGAGAAAGAATAGAACTTGGACGATGTATTGGAGAAGGCC
AATTTGGAGATGTACATCAAGGCATTTATATGAGTCCAGAGAATCCAGCT
TTGGCGGTTGCAATTAAAACATGTAAAAACTGTACTTCGGACAGCGTGAG
AGAGAAATTTCTTCAAGAAGCCTTAACAATGCGTCAGTTTGACCATCCTC
ATATTGTGAAGCTGATTGGAGTCATCACAGAGAATCCTGTCTGGATAATC
ATGGAGCTGTGCACACTTGGAGAGCTGAGGTCATTTTTGCAAGTAAGGAA
ATACAGTTTGGATCTAGCATCTTTGATCCTGTATGCCTATCAGCTTAGTA
CAGCTCTTGCATATCTAGAGAGCAAAAGATTTGTACACAGGGACATTGCT
GCTCGGAATGTTCTGGTGTCCTCAAATGATTGTGTAAAATTAGGAGACTT
TGGATTATCCCGATATATGGAAGATAGTACTTACTACAAAGCTTCCAAAG
GAAAATTGCCTATTAAATGGATGGCTCCAGAGTCAATCAATTTTCGACGT
TTTACCTCAGCTAGTGACGTATGGATGTTTGGTGTGTGTATGTGGGAGAT
ACTGATGCATGGTGTGAAGCCTTTTCAAGGAGTGAAGAACAATGATGTAA
TCGGTCGAATTGAAAATGGGGAAAGATTACCAATGCCTCCAAATTGTCCT
CCTACCCTCTACAGCCTTATGACGAAATGCTGGGCCTATGACCCCAGCAG
GCGGCCCAGGTTTACTGAACTTAAAGCTCAGCTCAGCACAATCCTGGAGG
AAGAGAAGGCTCAGCAAGAAGAGCGCATGAGGATGGAGTCCAGAAGACAG
GCCACAGTGTCCTGGGACTCCGGAGGGTCTGATGAAGCACCGCCCAAGCC
CAGCAGACCGGGTTATCCCAGTCCGAGGTCCAGCGAAGGATTTTATCCCA
GCCCACAGCACATGGTACAAACCAATCATTACCAGGACTCTACAGTATTG
GACCTGCGAGGGATTGGGCAAGTGTTGCCAACCCATCTGATGGAAGAGCG
TCTAATCCGACAGCAACAGGAAATGGAAGAAGATCAGCGCTGGCTGGAAA
AAGAGGAAAGATTTCTGAAACCTGATGTGAGACTCTCTCGAGGCAGTATT
GACAGGGAGGATGGAAGTCTTCAGGGTCCGATTGGAAACCAACATATATA
TCAGCCTGTGGGTAAACCAGATCCTGCAGCTCCACCAAAGAAACCGCCTC
GCCCTGGAGCTCCCGGTCATCTGGGAAGCCTTGCCAGCCTCAGCAGCCCT
GCTGACAGCTACAACGAGGGTGTCAAGCTTCAGCCCCAGGAAATCAGCCC
CCCTCCTACTGCCAACCTGGACCGGTCGAATGATAAGGTGTACGAGAATG
TGACGGGCCTGGTGAAAGCTGTCATCGAGATGTCCAGTAAAATCCAGCCA
GCCCCACCAGAGGAGTATGTCCCTATGGTGAAGGAAGTCGGCTTGGCCCT
GAGGACATTATTGGCCACTGTGGATGAGACCATTCCCCTCCTACCAGCCA
GCACCCACCGAGAGATTGAGATGGCACAGAAGCTATTGAACTCTGACCTG
GGTGAGCTCATCAACAAGATGAAACTGGCCCAGCAGTATGTCATGACCAG
CCTCCAGCAAGAGTACAAAAAGCAAATGCTGACTGCTGCTCACGCCCTGG
CTGTGGATGCCAAAAACTTACTCGATGTCATTGACCAAGCAAGACTGAAA
ATGCTTGGGCAGACGAGACCACACTGAGCGGCCGC

Amino Acid Sequence

MSPILGYWKIKGLVQPTRLLLEYLEEKYEEHLYERDEGDKWRNKKFELGL
EFPNLPYYIDGDVKLTQSMAIIRYIADKHNMLGGCPKERAEISMLEGAVL
DIRYGVSRIAYSKDFETLKVDFLSKLPEMLKMFEDRLCHKTYLNGDHVTH
PDFMLYDALDVVLYMDPMCLDAFPKLVCFKKRIEAIPQIDKYLKSSKYIA
WPLQGWQATFGGGDHPPKSDLEVLFQGPLGSMAAAYLDPNLNHTPNSSTK
THLGTGMERSPGAMERVLKVFHYFESNSEPTTWASIIRHGDATDVRGIIQ
KIVDSHKVKHVACYGFRLSHLRSEEVHWLHVDMGVSSVREKYELAHPPEE
WKYELRIRYLPKGFLNQFTEDKPTLNFFYQQVKSDYMLEIADQVDQEIAL
KLGCLEIRRSYWEMRGNALEKKSNYEVLEKDVGLKRFFPKSLLDSVKAKT
LRKLIQQTFRQFANLNREESILKFFEILSPVYRFDKECFKCALGSSWIIS
VELAIGPEEGISYLTDKGCNPTHLADFTQVQTIQYSNSEDKDRKGMLQLK
IAGAPEPLTVTAPSLTIAENMADLIDGYCRLVNGTSQSFIIRPQKEGERA
LPSIPKLANSEKQGMRTHAVSVSETDDYAEIIDEEDTYTMPSTRDYEIQR
ERIELGRCIGEGQFGDVHQGIYMSPENPALAVAIKTCKNCTSDSVREKFL
QEALTMRQFDHPHIVKLIGVITENPVWIIMELCTLGELRSFLQVRKYSLD
LASLILYAYQLSTALAYLESKRFVHRDIAARNVLVSSNDCVKLGDFGLSR
YMEDSTYYKASKGKLPIKWMAPESINFRRFTSASDVWMFGVCMWEILMHG
VKPFQGVKNNDVIGRIENGERLPMPPNCPPTLYSLMTKCWAYDPSRRPRF
TELKAQLSTILEEEKAQQEERMRMESRRQATVSWDSGGSDEAPPKPSRPG
YPSPRSSEGFYPSPQHMVQTNHYQDSTVLDLRGIGQVLPTHLMEERLIRQ
QQEMEEDQRWLEKEERFLKPDVRLSRGSIDREDGSLQGPIGNQHIYQPVG
KPDPAAPPKKPPRPGAPGHLGSLASLSSPADSYNEGVKLQPQEISPPPTA
NLDRSNDKVYENVTGLVKAVIEMSSKIQPAPPEEYVPMVKEVGLALRTLL
ATVDETIPLLPASTHREIEMAQKLLNSDLGELINKMKLAQQYVMTSLQQE
YKKQMLTAAHALAVDAKNLLDVIDQARLKMLGQTRPH*

• Vector Type: Bacterial
• Antibiotic: Amp
• DU Number: DU36421
• Unit Source: Protein Phosphorylation and Ubiquitylation Unit
• Location: PPU
• MTA: Dundee
• Notes: *Difference compared with NM_153831:; Delta V744-E789 – (derives from EST); Bacterial construct to express GST N terminal fusion ; ; Estimated MW of expressed protein : 141072 Daltons