cDNA Clone BRCA1

• Plasmid: pGEX6P-3-BRCA1
• Parent Plasmid: pGEX6P3
• Expressed: GST-BRCA1
• Genbank: AAA73985.1
• Tag: GST
• Species: Human

Sequence of Insert

GGATCCATGGATTTATCTGCTCTTCGCGTTGAAGAAGTACAAAATGTCAT
TAATGCTATGCAGAAAATCTTAGAGTGTCCCATCTGTCTGGAGTTGATCA
AGGAACCTGTCTCCACAAAGTGTGACCACATATTTTGCAAATTTTGCATG
CTGAAACTTCTCAACCAGAAGAAAGGGCCTTCACAGTGTCCTTTATGTAA
GAATGATATAACCAAAAGGAGCCTACAAGAAAGTACGAGATTTAGTCAAC
TTGTTGAAGAGCTATTGAAAATCATTTGTGCTTTTCAGCTTGACACAGGT
TTGGAGTATGCAAACAGCTATAATTTTGCAAAAAAGGAAAATAACTCTCC
TGAACATCTAAAAGATGAAGTTTCTATCATCCAAAGTATGGGCTACAGAA
ACCGTGCCAAAAGACTTCTACAGAGTGAACCCGAAAATCCTTCCTTGCAG
GAAACCAGTCTCAGTGTCCAACTCTCTAACCTTGGAACTGTGAGAACTCT
GAGGACAAAGCAGCGGATACAACCTCAAAAGACGTCTGTCTACATTGAAT
TGGGATCTGATTCTTCTGAAGATACCGTTAATAAGGCAACTTATTGCAGT
GTGGGAGATCAAGAATTGTTACAAATCACCCCTCAAGGAACCAGGGATGA
AATCAGTTTGGATTCTGCAAAAAAGGCTGCTTGTGAATTTTCTGAGACGG
ATGTAACAAATACTGAACATCATCAACCCAGTAATAATGATTTGAACACC
ACTGAGAAGCGTGCAGCTGAGAGGCATCCAGAAAAGTATCAGGGTAGTTC
TGTTTCAAACTTGCATGTGGAGCCATGTGGCACAAATACTCATGCCAGCT
CATTACAGCATGAGAACAGCAGTTTATTACTCACTAAAGACAGAATGAAT
GTAGAAAAGGCTGAATTCTGTAATAAAAGCAAACAGCCTGGCTTAGCAAG
GAGCCAACATAACAGATGGGCTGGAAGTAAGGAAACATGTAATGATAGGC
GGACTCCCAGCACAGAAAAAAAGGTAGATCTGAATGCTGATCCCCTGTGT
GAGAGAAAAGAATGGAATAAGCAGAAACTGCCATGCTCAGAGAATCCTAG
AGATACTGAAGATGTTCCTTGGATAACACTAAATAGCAGCATTCAGAAAG
TTAATGAGTGGTTTTCCAGAAGTGATGAACTGTTAGGTTCTGATGACTCA
CATGATGGGGAGTCTGAATCAAATGCCAAAGTAGCTGATGTATTGGACGT
TCTAAATGAGGTAGATGAATATTCTGGTTCTTCAGAGAAAATAGACTTAC
TGGCCAGTGATCCTCATGAGGCTTTAATATGTAAAAGTGAAAGAGTTCAC
TCCAAATCAGTAGAGAGTAATATTGAAGACAAAATATTTGGGAAAACCTA
TCGGAAGAAGGCAAGCCTCCCCAACTTAAGCCATGTAACTGAAAATCTAA
TTATAGGAGCATTTGTTACTGAGCCACAGATAATACAAGAGCGTCCCCTC
ACAAATAAATTAAAGCGTAAAAGGAGACCTACATCAGGCCTTCATCCTGA
GGATTTTATCAAGAAAGCAGATTTGGCAGTTCAAAAGACTCCTGAAATGA
TAAATCAGGGAACTAACCAAACGGAGCAGAATGGTCAAGTGATGAATATT
ACTAATAGTGGTCATGAGAATAAAACAAAAGGTGATTCTATTCAGAATGA
GAAAAATCCTAACCCAATAGAATCACTCGAAAAAGAATCTGCTTTCAAAA
CGAAAGCTGAACCTATAAGCAGCAGTATAAGCAATATGGAACTCGAATTA
AATATCCACAATTCAAAAGCACCTAAAAAGAATAGGCTGAGGAGGAAGTC
TTCTACCAGGCATATTCATGCGCTTGAACTAGTAGTCAGTAGAAATCTAA
GCCCACCTAATTGTACTGAATTGCAAATTGATAGTTGTTCTAGCAGTGAA
GAGATAAAGAAAAAAAAGTACAACCAAATGCCAGTCAGGCACAGCAGAAA
CCTACAACTCATGGAAGGTAAAGAACCTGCAACTGGAGCCAAGAAGAGTA
ACAAGCCAAATGAACAGACAAGTAAAAGACATGACAGTGATACTTTCCCA
GAGCTGAAGTTAACAAATGCACCTGGTTCTTTTACTAAGTGTTCAAATAC
CAGTGAACTTAAAGAATTTGTCAATCCTAGCCTTCCAAGAGAAGAAAAAG
AAGAGAAACTAGAAACAGTTAAAGTGTCTAATAATGCTGAAGACCCCAAA
GATCTCATGTTAAGTGGAGAAAGGGTTTTGCAAACTGAAAGATCTGTAGA
GAGTAGCAGTATTTCACTGGTACCTGGTACTGATTATGGCACTCAGGAAA
GTATCTCGTTACTGGAAGTTAGCACTCTAGGGAAGGCAAAAACAGAACCA
AATAAATGTGTGAGTCAGTGTGCAGCATTTGAAAACCCCAAGGGACTAAT
TCATGGTTGTTCCAAAGATAATAGAAATGACACAGAAGGCTTTAAGTATC
CATTGGGACATGAAGTTAACCACAGTCGGGAAACAAGCATAGAAATGGAA
GAAAGTGAACTCGATGCTCAGTATTTGCAGAATACATTCAAGGTTTCAAA
GCGCCAGTCATTTGCTCTGTTTTCAAATCCAGGAAATGCAGAAGAGGAAT
GTGCAACATTCTCTGCCCACTCTGGGTCCTTAAAGAAACAAAGTCCAAAA
GTCACTTTTGAATGTGAACAAAAGGAAGAAAATCAAGGAAAGAATGAGTC
TAATATCAAGCCTGTACAGACAGTTAATATCACTGCAGGCTTTCCTGTGG
TTGGTCAGAAAGATAAGCCAGTTGATAATGCCAAATGTAGTATCAAAGGA
GGCTCTAGGTTTTGTCTATCATCTCAGTTCAGAGGCAACGAAACTGGACT
CATTACTCCAAATAAACATGGACTTTTACAAAACCCATATCGTATACCAC
CACTTTTTCCCATCAAGTCATTTGTTAAAACTAAATGTAAGAAAAATCTG
CTAGAGGAAAACTTTGAGGAACATTCAATGTCACCTGAAAGAGAAATGGG
AAATGAGAACATTCCAAGTACAGTGAGCACAATTAGCCGTAATAACATTA
GAGAAAATGTTTTTAAAGGAGCCAGCTCAAGCAATATTAATGAAGTAGGT
TCCAGTACTAATGAAGTGGGCTCCAGTATTAATGAAATAGGTTCCAGTGA
TGAAAACATTCAAGCAGAACTAGGTAGAAACAGAGGGCCAAAATTGAATG
CTATGCTTAGATTAGGGGTTTTGCAACCTGAGGTCTATAAACAAAGTCTT
CCTGGAAGTAATTGTAAGCATCCTGAAATAAAAAAGCAAGAATATGAAGA
AGTAGTTCAGACTGTTAATACAGATTTCTCTCCATATCTGATTTCAGATA
ACTTAGAACAGCCTATGGGAAGTAGTCATGCATCTCAGGTTTGTTCTGAG
ACACCTGATGACCTGTTAGATGATGGTGAAATAAAGGAAGATACTAGTTT
TGCTGAAAATGACATTAAGGAAAGTTCTGCTGTTTTTAGCAAAAGCGTCC
AGAGAGGAGAGCTTAGCAGGAGTCCTAGCCCTTTCACCCATACACATTTG
GCTCAGGGTTACCGAAGAGGGGCCAAGAAATTAGAGTCCTCAGAAGAGAA
CTTATCTAGTGAGGATGAAGAGCTTCCCTGCTTCCAACACTTGTTATTTG
GTAAAGTAAACAATATACCTTCTCAGTCTACTAGGCATAGCACCGTTGCT
ACCGAGTGTCTGTCTAAGAACACAGAGGAGAATTTATTATCATTGAAGAA
TAGCTTAAATGACTGCAGTAACCAGGTAATATTGGCAAAGGCATCTCAGG
AACATCACCTTAGTGAGGAAACAAAATGTTCTGCTAGCTTGTTTTCTTCA
CAGTGCAGTGAATTGGAAGACTTGACTGCAAATACAAACACCCAAGACCC
TTTCTTGATTGGTTCTTCCAAACAAATGAGGCATCAGTCTGAAAGCCAGG
GAGTTGGTCTGAGTGACAAGGAATTGGTTTCAGATGATGAAGAAAGAGGA
ACGGGCTTGGAAGAAAATAATCAAGAAGAGCAAAGCATGGATTCAAACTT
AGGTGAAGCAGCATCTGGGTGTGAGAGTGAAACAAGCGTCTCTGAAGACT
GCTCAGGGCTATCCTCTCAGAGTGACATTTTAACCACTCAGCAGAGGGAT
ACCATGCAACATAACCTGATAAAGCTCCAGCAGGAAATGGCTGAACTAGA
AGCTGTGTTAGAACAGCATGGGAGCCAGCCTTCTAACAGCTACCCTTCCA
TCATAAGTGACTCCTCTGCCCTTGAGGACCTGCGAAATCCAGAACAAAGC
ACATCAGAAAAAGATTCGCATATACATGGCCAAAGGGACAACTCCATGTT
TTCTAAAAGGCCTAGAGAACATATATCAGTATTAACTTCACAGAAAAGTA
GTGAATACCCTATAAGCCAGAATCCAGAAGGCCTTTCTGCTGACAAGTTT
GAGGTGTCTGCAGATAGTTCTACCAGTAAAAATAAAGAACCAGGAGTGGA
AAGGTCATCCCCTTCTAAATGCCCATCATTAGATGATAGGTGGTACATGC
ACAGTTGCTCTGGGAGTCTTCAGAATAGAAACTACCCATCTCAAGAGGAG
CTCATTAAGGTTGTTGATGTGGAGGAGCAACAGCTGGAAGAGTCTGGGCC
ACACGATTTGACGGAAACATCTTACTTGCCAAGGCAAGATCTAGAGGGAA
CCCCTTACCTGGAATCTGGAATCAGCCTCTTCTCTGATGACCCTGAATCT
GATCCTTCTGAAGACAGAGCCCCAGAGTCAGCTCGTGTTGGCAACATACC
ATCTTCAACCTCTGCATTGAAATTTCCCCAATTGAAAGTTGCAGAATCTG
CCCAGGGTCCAGCTGCTGCTCATACTACTGATACTGCTGGGTATAATGCA
ATGGAAGAAAGTGTGAGCAGGGAGAAGCCAGAATTGACAGCTTCAACAGA
AAGGGTCAACAAAAGAATGTCCATGGTGGTGTCTGGCCTGACCCCAGAAG
AATTTATGCTCGTGTACAAGTTTGCCAGAAAACACCACATCACTTTAACT
AATCTAATTACTGAAGAGACTACTCATGTTGTTATGAAAACAGATGCTGA
GTTTGTGTGTGAACGGACACTGAAATATTTTCTAGGAATTGCGGGAGGAA
AATGGGTAGTTAGCTATTTCTGGGTGACCCAGTCTATTAAAGAAAGAAAA
ATGCTGAATGAGCATGATTTTGAAGTCAGAGGAGATGTGGTCAATGGAAG
AAACCACCAAGGTCCAAAGCGAGCAAGAGAATCCCAGGACAGAAAGATCT
TCAGGGGGCTAGAAATCTGTTGCTATGGGCCCTTCACCAACATGCCCACA
GATCAACTGGAATGGATGGTACAGCTGTGTGGTGCTTCTGTGGTGAAGGA
GCTTTCATCATTCACCCTTGGCACAGGTGTCCACCCAATTGTGGTTGTGC
AGCCAGATGCCTGGACAGAGGACAATGGCTTCCATGCAATTGGGCAGATG
TGTGAGGCACCTGTGGTGACCCGAGAGTGGGTGTTGGACAGTGTAGCACT
CTACCAGTGCCAGGAGCTGGACACCTACCTGATACCCCAGATCCCCCACA
GCCACTACTGAGCGGCCGC

Amino Acid Sequence

MSPILGYWKIKGLVQPTRLLLEYLEEKYEEHLYERDEGDKWRNKKFELGL
EFPNLPYYIDGDVKLTQSMAIIRYIADKHNMLGGCPKERAEISMLEGAVL
DIRYGVSRIAYSKDFETLKVDFLSKLPEMLKMFEDRLCHKTYLNGDHVTH
PDFMLYDALDVVLYMDPMCLDAFPKLVCFKKRIEAIPQIDKYLKSSKYIA
WPLQGWQATFGGGDHPPKSDLEVLFQGPLGSMDLSALRVEEVQNVINAMQ
KILECPICLELIKEPVSTKCDHIFCKFCMLKLLNQKKGPSQCPLCKNDIT
KRSLQESTRFSQLVEELLKIICAFQLDTGLEYANSYNFAKKENNSPEHLK
DEVSIIQSMGYRNRAKRLLQSEPENPSLQETSLSVQLSNLGTVRTLRTKQ
RIQPQKTSVYIELGSDSSEDTVNKATYCSVGDQELLQITPQGTRDEISLD
SAKKAACEFSETDVTNTEHHQPSNNDLNTTEKRAAERHPEKYQGSSVSNL
HVEPCGTNTHASSLQHENSSLLLTKDRMNVEKAEFCNKSKQPGLARSQHN
RWAGSKETCNDRRTPSTEKKVDLNADPLCERKEWNKQKLPCSENPRDTED
VPWITLNSSIQKVNEWFSRSDELLGSDDSHDGESESNAKVADVLDVLNEV
DEYSGSSEKIDLLASDPHEALICKSERVHSKSVESNIEDKIFGKTYRKKA
SLPNLSHVTENLIIGAFVTEPQIIQERPLTNKLKRKRRPTSGLHPEDFIK
KADLAVQKTPEMINQGTNQTEQNGQVMNITNSGHENKTKGDSIQNEKNPN
PIESLEKESAFKTKAEPISSSISNMELELNIHNSKAPKKNRLRRKSSTRH
IHALELVVSRNLSPPNCTELQIDSCSSSEEIKKKKYNQMPVRHSRNLQLM
EGKEPATGAKKSNKPNEQTSKRHDSDTFPELKLTNAPGSFTKCSNTSELK
EFVNPSLPREEKEEKLETVKVSNNAEDPKDLMLSGERVLQTERSVESSSI
SLVPGTDYGTQESISLLEVSTLGKAKTEPNKCVSQCAAFENPKGLIHGCS
KDNRNDTEGFKYPLGHEVNHSRETSIEMEESELDAQYLQNTFKVSKRQSF
ALFSNPGNAEEECATFSAHSGSLKKQSPKVTFECEQKEENQGKNESNIKP
VQTVNITAGFPVVGQKDKPVDNAKCSIKGGSRFCLSSQFRGNETGLITPN
KHGLLQNPYRIPPLFPIKSFVKTKCKKNLLEENFEEHSMSPEREMGNENI
PSTVSTISRNNIRENVFKGASSSNINEVGSSTNEVGSSINEIGSSDENIQ
AELGRNRGPKLNAMLRLGVLQPEVYKQSLPGSNCKHPEIKKQEYEEVVQT
VNTDFSPYLISDNLEQPMGSSHASQVCSETPDDLLDDGEIKEDTSFAEND
IKESSAVFSKSVQRGELSRSPSPFTHTHLAQGYRRGAKKLESSEENLSSE
DEELPCFQHLLFGKVNNIPSQSTRHSTVATECLSKNTEENLLSLKNSLND
CSNQVILAKASQEHHLSEETKCSASLFSSQCSELEDLTANTNTQDPFLIG
SSKQMRHQSESQGVGLSDKELVSDDEERGTGLEENNQEEQSMDSNLGEAA
SGCESETSVSEDCSGLSSQSDILTTQQRDTMQHNLIKLQQEMAELEAVLE
QHGSQPSNSYPSIISDSSALEDLRNPEQSTSEKDSHIHGQRDNSMFSKRP
REHISVLTSQKSSEYPISQNPEGLSADKFEVSADSSTSKNKEPGVERSSP
SKCPSLDDRWYMHSCSGSLQNRNYPSQEELIKVVDVEEQQLEESGPHDLT
ETSYLPRQDLEGTPYLESGISLFSDDPESDPSEDRAPESARVGNIPSSTS
ALKFPQLKVAESAQGPAAAHTTDTAGYNAMEESVSREKPELTASTERVNK
RMSMVVSGLTPEEFMLVYKFARKHHITLTNLITEETTHVVMKTDAEFVCE
RTLKYFLGIAGGKWVVSYFWVTQSIKERKMLNEHDFEVRGDVVNGRNHQG
PKRARESQDRKIFRGLEICCYGPFTNMPTDQLEWMVQLCGASVVKELSSF
TLGTGVHPIVVVQPDAWTEDNGFHAIGQMCEAPVVTREWVLDSVALYQCQ
ELDTYLIPQIPHSHY*

• Vector Type: Bacterial
• Antibiotic: Amp
• DU Number: DU36316
• Unit Source: Protein Phosphorylation and Ubiquitylation Unit
• Location: PPU
• MTA: Dundee
• Notes: Bacterial construct to express GST N terminal fusion ; ; Estimated MW of expressed protein : 237217 Daltons