cDNA Clone USP22

• Plasmid: pFB.HTb USP22
• Parent Plasmid: pFastBac HTb
• Expressed: 6His USP22
• Genbank: NM_015276.1 GI:150010638
• Tag: 6His
• Species: Human

Sequence of Insert

ggatccatggtgtcccggccagagcccgagggcgaggccatggacgccga
gctggcggtagcgccgccgggctgctcgcacctgggcagcttcaaggtgg
acaactggaagcagaacctgcgggccatctaccagtgcttcgtgtggagc
ggcacggctgaggcccgcaagcgcaaggccaagtcctgtatctgccatgt
ctgtggcgtccacctcaacaggctgcattcctgcctctactgtgtcttct
tcggctgtttcacaaagaagcatattcacgagcatgcgaaggcgaagcgg
cacaacctggccattgatctgatgtacggaggcatctactgttttctgtg
ccaggactacatctatgacaaagacatggaaataatcgccaaggaggagc
agcgaaaagcttggaaaatgcaaggcgttggagagaagttttcaacttgg
gaaccaaccaaacgggagcttgaactgctgaagcacaacccgaaaaggag
aaagatcacctcgaactgcaccataggtctgcgtgggctgatcaaccttg
ggaacacatgcttcatgaactgcatcgtgcaggccctgacccacacgcca
cttctgcgggacttcttcctgtctgacaggcaccgctgtgagatgcagag
ccccagctcctgtctggtctgtgagatgtcctcactgtttcaggagtttt
actctggacaccggtcccctcacatcccgtataagttgctgcacctggtg
tggacccacgcgaggcacctagcaggctacgagcagcaggacgcccacga
gttcctcatcgcggccctggacgtgctccaccgacactgcaaaggtgatg
acaatgggaagaaggccaacaaccccaaccactgcaactgcatcatagac
cagatcttcacaggcgggttgcagtcagacgtcacctgccaagtctgcca
tggagtctccaccaccatcgaccccttctgggacatcagcttggatctcc
ccggctcttccaccccattctggcccctgagcccagggagcgagggcaac
gtggtaaacggggaaagccacgtgtcgggaaccaccacgctcacggactg
cctgcgacgattcaccagaccagagcacttgggcagcagcgccaagatca
agtgcagcggttgccatagctaccaggagtccacaaagcagctcactatg
aagaaactgcccatcgtagcctgttttcatctcaaacgatttgaacactc
agccaagctgcggcggaagatcaccacgtatgtgtccttccccctggagc
tggacatgacccctttcatggcctccagcaaagagagcaggatgaatgga
cagtaccagcagcccacggacagtctcaacaatgacaacaagtattccct
gtttgctgttgttaaccatcaagggaccttggagagtggccactacacca
gctttatccggcagcacaaagaccagtggttcaagtgtgacgatgccatc
atcaccaaggccagcatcaaggacgtcctggacagcgaagggtacttgct
gttctatcacaaacagttcctggaatacgagtaggtcgactcgagcggcc
gc

Amino Acid Sequence

MSYYHHHHHHDYDIPTTENLYFQGAMGSMVSRPEPEGEAMDAELAVAPPG
CSHLGSFKVDNWKQNLRAIYQCFVWSGTAEARKRKAKSCICHVCGVHLNR
LHSCLYCVFFGCFTKKHIHEHAKAKRHNLAIDLMYGGIYCFLCQDYIYDK
DMEIIAKEEQRKAWKMQGVGEKFSTWEPTKRELELLKHNPKRRKITSNCT
IGLRGLINLGNTCFMNCIVQALTHTPLLRDFFLSDRHRCEMQSPSSCLVC
EMSSLFQEFYSGHRSPHIPYKLLHLVWTHARHLAGYEQQDAHEFLIAALD
VLHRHCKGDDNGKKANNPNHCNCIIDQIFTGGLQSDVTCQVCHGVSTTID
PFWDISLDLPGSSTPFWPLSPGSEGNVVNGESHVSGTTTLTDCLRRFTRP
EHLGSSAKIKCSGCHSYQESTKQLTMKKLPIVACFHLKRFEHSAKLRRKI
TTYVSFPLELDMTPFMASSKESRMNGQYQQPTDSLNNDNKYSLFAVVNHQ
GTLESGHYTSFIRQHKDQWFKCDDAIITKASIKDVLDSEGYLLFYHKQFL
EYE*

• Vector Type: Baculovirus
• Antibiotic: Amp
• DU Number: DU36274
• Unit Source: Protein Phosphorylation and Ubiquitylation Unit
• Location: PPU
• MTA: Dundee
• Notes: Baculovirus expression plasmid with N terminal 6His tag; ; Estimated MW of expressed protein : 63330 Daltons