cDNA Clone CDKL5

• Plasmid: pGEX6P-CDKL5
• Parent Plasmid: pGEX6P3
• Expressed: GST-CDKL5
• Genbank: NM_003159.2 GI:83367066*
• Tag: GST
• Species: Human

Sequence of Insert

GGATCCATGAAGATTCCTAACATTGGTAATGTGATGAATAAATTTGAGAT
CCTTGGGGTTGTAGGTGAAGGAGCCTATGGAGTTGTACTTAAATGCAGAC
ACAAGGAAACACATGAAATTGTGGCGATCAAGAAATTCAAGGACAGTGAA
GAAAATGAAGAAGTCAAAGAAACGACTTTACGAGAGCTTAAAATGCTTCG
GACTCTCAAGCAGGAAAACATTGTGGAGTTGAAGGAAGCATTTCGTCGGA
GGGGAAAGTTGTACTTGGTGTTTGAGTATGTTGAAAAAAATATGCTCGAA
TTGCTGGAAGAAATGCCAAATGGAGTTCCACCTGAGAAAGTAAAAAGCTA
CATCTATCAGCTAATCAAGGCTATTCACTGGTGCCATAAGAATGATATTG
TCCATCGAGATATAAAACCAGAAAATCTCTTAATCAGCCACAATGATGTC
CTAAAACTGTGTGACTTTGGTTTTGCTCGTAATCTGTCAGAAGGCAATAA
TGCTAATTACACAGAGTACGTTGCCACCAGATGGTATCGGTCCCCAGAAC
TCTTACTTGGCGCTCCCTATGGAAAGTCCGTGGACATGTGGTCGGTGGGC
TGTATTCTTGGGGAGCTTAGCGATGGACAGCCTTTATTTCCTGGAGAAAG
TGAAATTGACCAACTTTTTACTATTCAGAAGGTGCTAGGACCACTTCCAT
CTGAGCAGATGAAGCTTTTCTACAGTAATCCTCGCTTCCATGGGCTCCGG
TTTCCAGCTGTTAACCATCCTCAGTCCTTGGAAAGAAGATACCTTGGAAT
TTTGAATAGTGTTCTACTTGACCTAATGAAGAATTTACTGAAGTTGGACC
CAGCTGACAGATACTTGACAGAACAGTGTTTGAATCACCCTACATTTCAA
ACCCAGAGACTTCTGGATCGTTCTCCTTCAAGGTCAGCAAAAAGAAAACC
TTACCATGTGGAAAGCAGCACATTGTCTAATAGAAACCAAGCCGGCAAAA
GCACTGCTTTGCAGTCTCACCACAGATCTAACAGCAAGGACATCCAGAAC
CTGAGTGTAGGCCTGCCCCGGGCTGACGAAGGTCTCCCTGCCAATGAAAG
CTTCCTAAATGGAAACCTTGCTGGAGCTAGTCTTAGTCCACTGCACACCA
AAACCTACCAAGCAAGCAGCCAGCCTGGGTCTACCAGCAAAGATCTCACC
AACAACAACATACCACACCTTCTTAGCCCAAAAGAAGCCAAGTCAAAAAC
AGAGTTTGATTTTAATATTGACCCAAAGCCTTCAGAAGGCCCAGGGACAA
AGTACCTCAAGTCAAACAGCAGATCTCAGCAGAACCGCCACTCATTCATG
GAAAGCTCTCAAAGCAAAGCTGGGACACTGCAGCCCAATGAAAAGCAGAG
TCGGCATAGCTATATTGACACAATTCCCCAGTCCTCTAGGAGTCCCTCCT
ACAGGACCAAGGCCAAAAGCCATGGGGCACTGAGTGACTCCAAGTCTGTG
AGCAACCTTTCTGAAGCCAGGGCCCAAATTGCGGAGCCCAGTACCAGTAG
GTACTTCCCATCTAGCTGCTTAGACTTGAATTCTCCCACCAGCCCAACCC
CCACCAGACACAGTGACACGAGAACTTTGCTCAGCCCTTCTGGAAGAAAT
AACCGAAATGAGGGAACGCTGGACTCACGTCGAACCACAACCAGACATTC
TAAGACGATGGAGGAATTGAAGCTGCCGGAGCACATGGACAGTAGCCATT
CCCATTCACTGTCTGCACCTCACGAATCTTTTTCTTATGGACTGGGCTAC
ACCAGCCCCTTTTCTTCCCAGCAACGTCCTCATAGGCATTCTATGTATGT
GACCCGTGACAAAGTGAGAGCCAAGGGCTTGGATGGAAGCTTGAGCATAG
GGCAAGGGATGGCAGCTAGAGCCAACAGCCTGCAACTCTTGTCACCCCAG
CCTGGAGAACAGCTCCCTCCAGAGATGACTGTGGCAAGATCTTCGGTCAA
AGAGACCTCCAGAGAAGGCACCTCTTCCTTCCATACACGCCAGAAGTCTG
AGGGTGGAGTGTATCATGACCCACACTCTGATGATGGCACAGCCCCCAAA
GAAAATAGACACCTATACAATGACCCTGTGCCAAGGAGAGTTGGTAGCTT
TTACAGAGTGCCATCTCCACGTCCAGACAATTCTTTCCATGAAAATAATG
TGTCAACTAGAGTTTCTTCTCTACCATCAGAGAGCAGTTCTGGAACCAAC
CACTCAAAAAGACAACCAGCATTCGATCCATGGAAAAGTCCTGAAAATAT
TAGTCATTCAGAGCAACTCAAGGAAAAAGAGAAGCAAGGATTTTTCAGGT
CAATGAAAAAGAAAAAGAAGAAATCTCAAACAGTACCCAATTCCGACAGC
CCTGATCTTCTGACGTTGCAGAAATCCATTCATTCTGCTAGCACTCCAAG
CAGCAGACCAAAGGAGTGGCGCCCCGAGAAGATCTCAGATCTGCAGACCC
AAAGCCAGCCATTAAAATCACTGCGCAAGTTGTTACATCTCTCTTCGGCC
TCAAATCACCCGGCTTCCTCAGATCCCCGCTTCCAGCCCTTAACAGCTCA
ACAAACCAAAAATTCCTTCTCAGAAATTCGGATTCACCCCCTGAGCCAGG
CCTCTGGCGGGAGCAGCAACATCCGGCAGGAACCCGCACCGAAGGGCAGG
CCAGCCCTCCAGCTGCCAGACGGTGGATGTGATGGCAGAAGACAGAGACA
CCATTCTGGACCCCAAGATAGACGCTTCATGTTAAGGACGACAGAACAAC
AAGGAGAATACTTCTGCTGTGGTGACCCAAAGAAGCCTCACACTCCGTGC
GTCCCAAACCGAGCCCTTCATCGTCCAATCTCCAGTCCTGCTCCCTATCC
AGTACTCCAGGTCCGAGGCACTTCCATGTGCCCGACACTCCAGGTCCGAG
GCACTGATGCTTTCAGCTGCCCAACCCAGCAATCCGGGTTCTCTTTCTTC
GTGAGACACGTTATGAGGGAAGCCCTGATTCACAGGGCCCAGGTAAACCA
AGCTGCGCTCCTGACATACCATGAGAATGCGGCACTGACGGGCAAGTGAG
CGGCCGC

Amino Acid Sequence

MSPILGYWKIKGLVQPTRLLLEYLEEKYEEHLYERDEGDKWRNKKFELGL
EFPNLPYYIDGDVKLTQSMAIIRYIADKHNMLGGCPKERAEISMLEGAVL
DIRYGVSRIAYSKDFETLKVDFLSKLPEMLKMFEDRLCHKTYLNGDHVTH
PDFMLYDALDVVLYMDPMCLDAFPKLVCFKKRIEAIPQIDKYLKSSKYIA
WPLQGWQATFGGGDHPPKSDLEVLFQGPLGSMKIPNIGNVMNKFEILGVV
GEGAYGVVLKCRHKETHEIVAIKKFKDSEENEEVKETTLRELKMLRTLKQ
ENIVELKEAFRRRGKLYLVFEYVEKNMLELLEEMPNGVPPEKVKSYIYQL
IKAIHWCHKNDIVHRDIKPENLLISHNDVLKLCDFGFARNLSEGNNANYT
EYVATRWYRSPELLLGAPYGKSVDMWSVGCILGELSDGQPLFPGESEIDQ
LFTIQKVLGPLPSEQMKLFYSNPRFHGLRFPAVNHPQSLERRYLGILNSV
LLDLMKNLLKLDPADRYLTEQCLNHPTFQTQRLLDRSPSRSAKRKPYHVE
SSTLSNRNQAGKSTALQSHHRSNSKDIQNLSVGLPRADEGLPANESFLNG
NLAGASLSPLHTKTYQASSQPGSTSKDLTNNNIPHLLSPKEAKSKTEFDF
NIDPKPSEGPGTKYLKSNSRSQQNRHSFMESSQSKAGTLQPNEKQSRHSY
IDTIPQSSRSPSYRTKAKSHGALSDSKSVSNLSEARAQIAEPSTSRYFPS
SCLDLNSPTSPTPTRHSDTRTLLSPSGRNNRNEGTLDSRRTTTRHSKTME
ELKLPEHMDSSHSHSLSAPHESFSYGLGYTSPFSSQQRPHRHSMYVTRDK
VRAKGLDGSLSIGQGMAARANSLQLLSPQPGEQLPPEMTVARSSVKETSR
EGTSSFHTRQKSEGGVYHDPHSDDGTAPKENRHLYNDPVPRRVGSFYRVP
SPRPDNSFHENNVSTRVSSLPSESSSGTNHSKRQPAFDPWKSPENISHSE
QLKEKEKQGFFRSMKKKKKKSQTVPNSDSPDLLTLQKSIHSASTPSSRPK
EWRPEKISDLQTQSQPLKSLRKLLHLSSASNHPASSDPRFQPLTAQQTKN
SFSEIRIHPLSQASGGSSNIRQEPAPKGRPALQLPDGGCDGRRQRHHSGP
QDRRFMLRTTEQQGEYFCCGDPKKPHTPCVPNRALHRPISSPAPYPVLQV
RGTSMCPTLQVRGTDAFSCPTQQSGFSFFVRHVMREALIHRAQVNQAALL
TYHENAALTGK*

• Vector Type: Bacterial
• Antibiotic: Amp
• DU Number: DU36153
• Unit Source: Protein Phosphorylation and Ubiquitylation Unit
• Location: PPU
• MTA: Dundee
• Notes: *Sequence difference compared with NM_003159.2 CDS:; t996c, t2118c (both silent); Bacterial construct to express GST N terminal fusion ; ; Estimated MW of expressed protein : 142358 Daltons