cDNA Clone GRK2

• Synonyms: BARK1, BETA-ARK1, FLJ16718, ADRBK1
• Plasmid: pGEX6P-GRK2
• Parent Plasmid: pGEX6P3
• Expressed: GST-GRK2
• Genbank: NM_001619.3 GI:148539875*
• Tag: GST
• Species: Human

Sequence of Insert

GGATCCATGGCGGACCTGGAGGCGGTGCTGGCCGACGTGAGCTACCTGAT
GGCCATGGAGAAGAGCAAGGCCACGCCGGCCGCGCGCGCCAGCAAGAAGA
TACTGCTGCCCGAGCCCAGCATCCGCAGTGTCATGCAGAAGTACCTGGAG
GACCGGGGCGAGGTGACCTTTGAGAAGATCTTTTCCCAGAAGCTGGGGTA
CCTGCTCTTCCGAGACTTCTGCCTGAACCACCTGGAGGAGGCCAGGCCCT
TGGTGGAATTCTATGAGGAGATCAAGAAGTACGAGAAGCTGGAGACGGAG
GAGGAGCGTGTGGCCCGCAGCCGGGAGATCTTCGACTCATACATCATGAA
GGAGCTGCTGGCCTGCTCGCATCCCTTCTCGAAGAGTGCCACTGAGCATG
TCCAAGGCCACCTGGGGAAGAAGCAGGTGCCTCCGGATCTCTTCCAGCCA
TACATCGAAGAGATTTGTCAAAACCTCCGAGGGGACGTGTTCCAGAAATT
CATTGAGAGCGATAAGTTCACACGGTTTTGCCAGTGGAAGAATGTGGAGC
TCAACATCCACCTGACCATGAATGACTTCAGCGTGCATCGCATCATTGGG
CGCGGGGGCTTTGGCGAGGTCTATGGGTGCCGGAAGGCTGACACAGGCAA
GATGTACGCCATGAAGTGCCTGGACAAAAAGCGCATCAAGATGAAGCAGG
GGGAGACCCTGGCCCTGAACGAGCGCATCATGCTCTCGCTCGTCAGCACT
GGGGACTGCCCATTCATTGTCTGCATGTCATACGCGTTCCACACGCCAGA
CAAGCTCAGCTTCATCCTGGACCTCATGAACGGTGGGGACCTGCACTACC
ACCTCTCCCAGCACGGGGTCTTCTCAGAGGCTGACATGCGCTTCTATGCG
GCCGAGATCATCCTGGGCCTGGAGCACATGCACAACCGCTTCGTGGTCTA
CCGGGACCTGAAGCCAGCCAACATCCTTCTGGACGAGCATGGCCACGTGC
GGATCTCGGACCTGGGCCTGGCCTGTGACTTCTCCAAGAAGAAGCCCCAT
GCCAGCGTGGGCACCCACGGGTACATGGCTCCGGAGGTCCTGCAGAAGGG
CGTGGCCTACGACAGCAGTGCCGACTGGTTCTCTCTGGGGTGCATGCTCT
TCAAGTTGCTGCGGGGGCACAGCCCCTTCCGGCAGCACAAGACCAAAGAC
AAGCATGAGATCGACCGCATGACGCTGACGATGGCCGTGGAGCTGCCCGA
CTCCTTCTCCCCTGAACTACGCTCCCTGCTGGAGGGGTTGCTGCAGAGGG
ATGTCAACCGGAGATTGGGCTGCCTGGGCCGAGGGGCTCAGGAGGTGAAA
GAGAGCCCCTTTTTCCGCTCCCTGGACTGGCAGATGGTCTTCTTGCAGAA
GTACCCTCCCCCGCTGATCCCCCCACGAGGGGAGGTGAACGCGGCCGACG
CCTTCGACATTGGCTCCTTCGATGAGGAGGACACAAAAGGAATCAAGTTA
CTGGACAGTGATCAGGAGCTCTACCGCAACTTCCCCCTCACCATCTCGGA
GCGGTGGCAGCAGGAGGTGGCAGAGACTGTCTTCGACACCATCAACGCTG
AGACAGACCGGCTGGAGGCTCGCAAGAAAGCCAAGAACAAGCAGCTGGGC
CATGAGGAAGACTACGCCCTGGGCAAGGACTGCATCATGCATGGCTACAT
GTCCAAGATGGGCAACCCCTTCCTGACCCAGTGGCAGCGGCGGTACTTCT
ACCTGTTCCCCAACCGCCTCGAGTGGCGGGGCGAGGGCGAGGCCCCGCAG
AGCCTGCTGACCATGGAGGAGATCCAGTCGGTGGAGGAGACGCAGATCAA
GGAGCGCAAGTGCCTGCTCCTCAAGATCCGCGGTGGGAAACAGTTCATTT
TGCAGTGCGATAGCGACCCTGAGCTGGTGCAGTGGAAGAAGGAGCTGCGC
GACGCCTACCGCGAGGCCCAGCAGCTGGTGCAGCGGGTGCCCAAGATGAA
GAACAAGCCGCGCTCGCCCGTGGTGGAGCTGAGCAAGGTGCCGCTGGTCC
AGCGCGGCAGTGCCAACGGCCTCTGAGCGGCCGC

Amino Acid Sequence

MSPILGYWKIKGLVQPTRLLLEYLEEKYEEHLYERDEGDKWRNKKFELGL
EFPNLPYYIDGDVKLTQSMAIIRYIADKHNMLGGCPKERAEISMLEGAVL
DIRYGVSRIAYSKDFETLKVDFLSKLPEMLKMFEDRLCHKTYLNGDHVTH
PDFMLYDALDVVLYMDPMCLDAFPKLVCFKKRIEAIPQIDKYLKSSKYIA
WPLQGWQATFGGGDHPPKSDLEVLFQGPLGSMADLEAVLADVSYLMAMEK
SKATPAARASKKILLPEPSIRSVMQKYLEDRGEVTFEKIFSQKLGYLLFR
DFCLNHLEEARPLVEFYEEIKKYEKLETEEERVARSREIFDSYIMKELLA
CSHPFSKSATEHVQGHLGKKQVPPDLFQPYIEEICQNLRGDVFQKFIESD
KFTRFCQWKNVELNIHLTMNDFSVHRIIGRGGFGEVYGCRKADTGKMYAM
KCLDKKRIKMKQGETLALNERIMLSLVSTGDCPFIVCMSYAFHTPDKLSF
ILDLMNGGDLHYHLSQHGVFSEADMRFYAAEIILGLEHMHNRFVVYRDLK
PANILLDEHGHVRISDLGLACDFSKKKPHASVGTHGYMAPEVLQKGVAYD
SSADWFSLGCMLFKLLRGHSPFRQHKTKDKHEIDRMTLTMAVELPDSFSP
ELRSLLEGLLQRDVNRRLGCLGRGAQEVKESPFFRSLDWQMVFLQKYPPP
LIPPRGEVNAADAFDIGSFDEEDTKGIKLLDSDQELYRNFPLTISERWQQ
EVAETVFDTINAETDRLEARKKAKNKQLGHEEDYALGKDCIMHGYMSKMG
NPFLTQWQRRYFYLFPNRLEWRGEGEAPQSLLTMEEIQSVEETQIKERKC
LLLKIRGGKQFILQCDSDPELVQWKKELRDAYREAQQLVQRVPKMKNKPR
SPVVELSKVPLVQRGSANGL*

• Vector Type: Bacterial
• Antibiotic: Amp
• DU Number: DU36115
• Unit Source: Protein Phosphorylation and Ubiquitylation Unit
• Location: PPU
• MTA: Dundee
• Notes: *Sequence differences compared with NM_001619.3 CDS:; c96a (silent); Bacterial construct to express GST N terminal fusion ; ; Estimated MW of expressed protein : 106395 Daltons