cDNA Clone FRK

• Synonyms: GTK, PTK5, RAK
• Plasmid: pGEX6P-2-FRK
• Parent Plasmid: pGEX6P2
• Expressed: GST-FRK
• Genbank: NM_002031.2 GI:31657133
• Tag: GST
• Species: Human

Sequence of Insert

GCGGCCGCGATGAGCAACATCTGTCAGAGGCTCTGGGAGTACCTAGAACC
CTATCTCCCCTGTTTGTCCACGGAGGCAGACAAGTCAACCGTGATTGAAA
ATCCAGGGGCCCTTTGCTCTCCCCAGTCACAGAGGCATGGCCACTACTTT
GTGGCTTTGTTTGATTACCAGGCTCGGACTGCTGAGGACTTGAGCTTCCG
AGCAGGTGACAAACTTCAAGTTCTGGACACTTTGCATGAGGGCTGGTGGT
TTGCCAGACACTTGGAGAAAAGACGAGATGGCTCCAGTCAGCAACTACAA
GGCTATATTCCTTCTAACTACGTGGCTGAGGACAGAAGCCTACAGGCAGA
GCCGTGGTTCTTTGGAGCAATCGGAAGATCAGATGCAGAGAAACAACTAT
TATATTCAGAAAACAAGACCGGTTCCTTTCTAATCAGAGAAAGTGAAAGC
CAAAAAGGAGAATTCTCTCTTTCAGTTTTAGATGGAGCAGTTGTAAAACA
CTACAGAATTAAAAGACTGGATGAAGGGGGATTTTTTCTCACGCGAAGAA
GAATCTTTTCAACACTGAACGAATTTGTGAGCCACTACACCAAGACAAGT
GACGGCCTGTGTGTCAAGCTGGGGAAACCATGCTTAAAGATCCAGGTCCC
AGCTCCATTTGATTTGTCGTATAAAACCGTGGACCAATGGGAGATAGACC
GCAACTCCATACAGCTTCTGAAGCGATTGGGATCTGGTCAGTTTGGCGAA
GTATGGGAAGGTCTGTGGAACAATACCACTCCAGTAGCAGTGAAAACATT
AAAACCAGGTTCAATGGATCCAAATGACTTCCTGAGGGAGGCACAGATAA
TGAAGAACCTAAGACATCCAAAGCTTATCCAGCTTTATGCTGTTTGCACT
TTAGAAGATCCAATTTATATTATTACAGAGTTGATGAGACATGGAAGTCT
GCAAGAATATCTCCAAAATGACACTGGATCAAAAATCCATCTGACTCAAC
AGGTAGACATGGCGGCACAGGTTGCCTCTGGAATGGCCTATCTGGAGTCT
CGGAACTACATTCACAGAGATCTGGCTGCCAGAAATGTCCTCGTTGGTGA
ACATAATATCTACAAAGTAGCAGATTTTGGACTTGCCAGAGTTTTTAAGG
TAGATAATGAAGACATCTATGAATCTAGACACGAAATAAAGCTGCCGGTG
AAGTGGACTGCGCCCGAAGCCATTCGTAGTAATAAATTCAGCATTAAGTC
CGATGTATGGTCATTTGGAATCCTTCTTTATGAAATCATTACTTATGGCA
AAATGCCTTACAGTGGTATGACAGGTGCCCAGGTAATCCAGATGTTGGCT
CAAAACTATAGACTTCCGCAACCATCCAACTGTCCACAGCAATTTTACAA
CATCATGTTGGAGTGCTGGAATGCAGAGCCTAAGGAACGACCTACATTTG
AGACACTGCGTTGGAAACTTGAAGACTATTTTGAAACAGACTCTTCATAT
TCAGATGCAAATAACTTCATAAGATGAGCGGCCGC

Amino Acid Sequence

MSPILGYWKIKGLVQPTRLLLEYLEEKYEEHLYERDEGDKWRNKKFELGL
EFPNLPYYIDGDVKLTQSMAIIRYIADKHNMLGGCPKERAEISMLEGAVL
DIRYGVSRIAYSKDFETLKVDFLSKLPEMLKMFEDRLCHKTYLNGDHVTH
PDFMLYDALDVVLYMDPMCLDAFPKLVCFKKRIEAIPQIDKYLKSSKYIA
WPLQGWQATFGGGDHPPKSDLEVLFQGPLGSPGIPGSTRAAAMSNICQRL
WEYLEPYLPCLSTEADKSTVIENPGALCSPQSQRHGHYFVALFDYQARTA
EDLSFRAGDKLQVLDTLHEGWWFARHLEKRRDGSSQQLQGYIPSNYVAED
RSLQAEPWFFGAIGRSDAEKQLLYSENKTGSFLIRESESQKGEFSLSVLD
GAVVKHYRIKRLDEGGFFLTRRRIFSTLNEFVSHYTKTSDGLCVKLGKPC
LKIQVPAPFDLSYKTVDQWEIDRNSIQLLKRLGSGQFGEVWEGLWNNTTP
VAVKTLKPGSMDPNDFLREAQIMKNLRHPKLIQLYAVCTLEDPIYIITEL
MRHGSLQEYLQNDTGSKIHLTQQVDMAAQVASGMAYLESRNYIHRDLAAR
NVLVGEHNIYKVADFGLARVFKVDNEDIYESRHEIKLPVKWTAPEAIRSN
KFSIKSDVWSFGILLYEIITYGKMPYSGMTGAQVIQMLAQNYRLPQPSNC
PQQFYNIMLECWNAEPKERPTFETLRWKLEDYFETDSSYSDANNFIR*

• Vector Type: Bacterial
• Antibiotic: Amp
• DU Number: DU36095
• Unit Source: Protein Phosphorylation and Ubiquitylation Unit
• Location: PPU
• MTA: Dundee
• Notes: Bacterial construct to express GST N terminal fusion ; ; Estimated MW of expressed protein : 86054 Daltons