cDNA Clone Grit (mouse)

• Plasmid: pGEX6P-2-(mouse) Grit M1-V200
• Parent Plasmid: pGEX6P2
• Expressed: GST-Grit M1-V200
• Genbank: NM_177379.3 GI:282847460
• Tag: GST
• Species: Mouse

Sequence of Insert

GGATCCATGAAGTCTCGCCCAACAAAACAGAAGCTAAAGCAGCGGGGCAT
CTTGAAAGAGAGAGTATTCGGTTGTGACTTGGGGGAGCACCTCCTCAATT
CTGGGTTTGAAGTGCCCCAGGTTCTTCAGAGCTGCACAGCGTTCATCGAG
AGGTATGGCATTGTGGATGGGATATATCGTCTCTCTGGCGTTGCCTCCAA
TATCCAGAGACTTCGTCATGAATTTGATTCTGAGCATGTCCCCGACCTGA
CAAAAGAACCTTATGTTCAAGACATCCATTCTGTGGGCTCCCTCTGTAAG
CTGTACTTCCGAGAACTCCCAAACCCTCTGCTCACCTACCAGCTATACGA
GAAGTTCTCTGATGCTGTTTCAGCAGCAACGGATGAAGAACGGTTGATAA
AAATCCATGATGTCATCCAGCAACTCCCCCCACCACACTATAGAACCCTG
GAGTTCCTGATGAGACACCTGTCTCTTCTAGCAGACTATTGCTCCATCAC
AAATATGCATGCGAAAAACCTTGCCATTGTGTGGGCACCAAACCTGCTGA
GATCAAAGCAGATAGAGTCAGCTTGCTTCAGTGGGACAGCAGCTTTCATG
GAAGTGTGAGCGGCCGC

Amino Acid Sequence

MSPILGYWKIKGLVQPTRLLLEYLEEKYEEHLYERDEGDKWRNKKFELGL
EFPNLPYYIDGDVKLTQSMAIIRYIADKHNMLGGCPKERAEISMLEGAVL
DIRYGVSRIAYSKDFETLKVDFLSKLPEMLKMFEDRLCHKTYLNGDHVTH
PDFMLYDALDVVLYMDPMCLDAFPKLVCFKKRIEAIPQIDKYLKSSKYIA
WPLQGWQATFGGGDHPPKSDLEVLFQGPLGSMKSRPTKQKLKQRGILKER
VFGCDLGEHLLNSGFEVPQVLQSCTAFIERYGIVDGIYRLSGVASNIQRL
RHEFDSEHVPDLTKEPYVQDIHSVGSLCKLYFRELPNPLLTYQLYEKFSD
AVSAATDEERLIKIHDVIQQLPPPHYRTLEFLMRHLSLLADYCSITNMHA
KNLAIVWAPNLLRSKQIESACFSGTAAFMEV*

• Vector Type: Bacterial
• Antibiotic: Amp
• DU Number: DU31635
• Unit Source: Protein Phosphorylation and Ubiquitylation Unit
• Location: PPU
• MTA: Dundee
• Notes: Bacterial construct to express GST N terminal fusion ; ; Estimated MW of expressed protein : 49717 Daltons