cDNA Clone 14-3-3 epsilon

• Synonyms: YWHAE
• Plasmid: pIRES2-mCherry 14-3-3 epsilon/pEGFP
• Parent Plasmid: pIRES2 mCherry/C-GFP
• Expressed: mCherry-14-3-3 epsilon/EGFP
• Genbank: NM_006761.4 GI:195546907
• Tag: GFP

Sequence of Insert

gaattctATGGATGATCGAGAGGATCTGGTGTACCAGGCGAAGCTGGCCGA
GCAGGCTGAGCGATACGACGAAATGGTGGAGTCAATGAAGAAAGTAGCAG
GGATGGATGTGGAGCTGACAGTTGAAGAAAGAAACCTCCTATCTGTTGCA
TATAAGAATGTGATTGGAGCTAGAAGAGCCTCCTGGAGAATAATCAGCAG
CATTGAACAGAAAGAAGAAAACAAGGGAGGAGAAGACAAGCTAAAAATGA
TTCGGGAATATCGGCAAATGGTTGAGACTGAGCTAAAGTTAATCTGTTGT
GACATTCTGGATGTACTGGACAAACACCTCATTCCAGCAGCTAACACTGG
CGAGTCCAAGGTTTTCTATTATAAAATGAAAGGGGACTACCACAGGTATC
TGGCAGAATTTGCCACAGGAAACGACAGGAAGGAGGCTGCGGAGAACAGC
CTAGTGGCTTATAAAGCTGCTAGTGATATTGCAATGACAGAACTTCCACC
AACGCATCCTATTCGCTTAGGTCTTGCTCTCAATTTTTCCGTATTCTACT
ACGAAATTCTTAATTCCCCTGACCGTGCCTGCAGGTTGGCAAAAGCAGCT
TTTGATGATGCAATTGCAGAACTGGATACGCTGAGTGAAGAAAGCTATAA
GGACTCTACACTTATCATGCAGTTGTTACGTGATAATCTGACACTATGGA
CTTCAGACATGCAGGGTGACGGTGAAGAGCAGAATAAAGAAGCGCTGCAG
GACGTGGAAGACGAAAATCAGTGAggatcc

Amino Acid Sequence

Amino acid sequence of mCherry-14-3-3 epsilon.
MVSKGEEDNMAIIKEFMRFKVHMEGSVNGHEFEIEGEGEGRPYEGTQTAK
LKVTKGGPLPFAWDILSPQFMYGSKAYVKHPADIPDYLKLSFPEGFKWER
VMNFEDGGVVTVTQDSSLQDGEFIYKVKLRGTNFPSDGPVMQKKTMGWEA
SSERMYPEDGALKGEIKQRLKLKDGGHYDAEVKTTYKAKKPVQLPGAYNV
NIKLDITSHNEDYTIVEQYERAEGRHSTGGMDELYKGSRAQASNSMDDRE
DLVYQAKLAEQAERYDEMVESMKKVAGMDVELTVEERNLLSVAYKNVIGA
RRASWRIISSIEQKEENKGGEDKLKMIREYRQMVETELKLICCDILDVLD
KHLIPAANTGESKVFYYKMKGDYHRYLAEFATGNDRKEAAENSLVAYKAA
SDIAMTELPPTHPIRLGLALNFSVFYYEILNSPDRACRLAKAAFDDAIAE
LDTLSEESYKDSTLIMQLLRDNLTLWTSDMQGDGEEQNKEALQDVEDENQ
*
Amino acid sequence of GFP
MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTLKFICT
TGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIF
FKDDGNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHN
VYIMADKQKNGIKVNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNH
YLSTQSALSKDPNEKRDHMVLLEFVTAAGITLGMDELYK*

• Vector Type: Mammalian
• Antibiotic: Kan
• DU Number: DU31612
• Unit Source: Protein Phosphorylation and Ubiquitylation Unit
• Location: PPU
• MTA: Dundee