cDNA Clone FGF-21

• Plasmid: pGEX6P-2-FGF-21 H29-S209(end)
• Parent Plasmid: pGEX6P2
• Expressed: GST-FGF-21 H29-S209(end)
• Genbank: NM_019113.2 GI:68215584*
• Tag: GST
• Species: Human

Sequence of Insert

GGATCCCACCCCATCCCTGACTCCAGTCCTCTCCTGCAATTCGGGGGCCA
AGTCCGGCAGCGGTACCTCTACACAGATGATGCCCAGCAGACAGAAGCCC
ACCTGGAGATCAGGGAGGATGGGACGGTGGGGGGCGCTGCTGACCAGAGC
CCCGAAAGTCTCCTGCAGCTGAAAGCCTTGAAGCCGGGAGTTATTCAAAT
CTTGGGAGTCAAGACATCCAGGTTCCTGTGCCAGCGGCCAGATGGGGCCC
TGTATGGATCGCTCCACTTTGACCCTGAGGCCTGCAGCTTCCGGGAGCTG
CTTCTTGAGGACGGATACAATGTTTACCAGTCCGAAGCCCACGGCCTCCC
GCTGCACCTGCCAGGGAACAAGTCCCCACACCGGGACCCTGCACCCCGAG
GACCAGCTCGCTTCCTGCCACTACCAGGCCTGCCCCCCGCACCCCCGGAG
CCACCCGGAATCCTGGCCCCCCAGCCCCCCGATGTGGGCTCCTCGGACCC
TCTGAGCATGGTGGGACCTTCCCAGGGCCGAAGCCCCAGCTACGCTTCCT
GAGCGGCCGC

Amino Acid Sequence

MSPILGYWKIKGLVQPTRLLLEYLEEKYEEHLYERDEGDKWRNKKFELGL
EFPNLPYYIDGDVKLTQSMAIIRYIADKHNMLGGCPKERAEISMLEGAVL
DIRYGVSRIAYSKDFETLKVDFLSKLPEMLKMFEDRLCHKTYLNGDHVTH
PDFMLYDALDVVLYMDPMCLDAFPKLVCFKKRIEAIPQIDKYLKSSKYIA
WPLQGWQATFGGGDHPPKSDLEVLFQGPLGSHPIPDSSPLLQFGGQVRQR
YLYTDDAQQTEAHLEIREDGTVGGAADQSPESLLQLKALKPGVIQILGVK
TSRFLCQRPDGALYGSLHFDPEACSFRELLLEDGYNVYQSEAHGLPLHLP
GNKSPHRDPAPRGPARFLPLPGLPPAPPEPPGILAPQPPDVGSSDPLSMV
GPSQGRSPSYAS*

• Vector Type: Bacterial
• Antibiotic: Amp
• DU Number: DU31574
• Unit Source: Protein Phosphorylation and Ubiquitylation Unit
• Location: PPU
• MTA: Dundee
• Notes: *Sequence difference compared with NM_019113.2 CDS:; t521c = L174P (present in lots of published seqs); Bacterial construct to express GST N terminal fusion ; ; Estimated MW of expressed protein : 46217 Daltons