cDNA Clone GRIPE

• Plasmid: pcDNA5-FRT/TO-HA GRIPE
• Parent Plasmid: pcDNA5 FRT/TO HA1
• Expressed: HA-GRIPE
• Genbank: NM_194301.2 GI:51230411*
• Tag: HA
• Species: Human

Sequence of Insert

AGATCTATGTTCTCCAAGAAGCCGCACGGGGACGTGAAGAAGTCCACCCA
GAAGGTGCTAGACACCAAGAAGGACGCACTGACTCGCCTCAAGCACCTGC
GCATCGTCATCGAGAATGCAGAATCTATTGATCTTAAACAGTTTTTCGAC
CAACATTTTTCACATATATACTATGTGTTCTTTGAAAATTTTGTGACTAT
TGAAGCTAGTCTTAAACAGAAAGGTCACAAGTCTCAAAGAGAGGAATTGG
ATGCTATACTTTTTATTTTTGAGAAAATTTTACAACTTCTTCCAGAAAGA
ATTCATCAGCGATGGCAGTTTCATAGTATTGGATTGATTTTAAAGAAGCT
ACTTCACACAGGAAACTCCTTAAAGATTAGGCGGGAAGGTGTTCGTCTTT
TCTTACTATGGTTGCAAGCTCTTCAGAATAACTGTAGCAAAGAACAGCTC
TGGATGTTTTCATGCTTAATCCCTGGATTTTCAGCACCACAGTCTGAACA
TGGACCTCGAACTTTAGATAATCTCATTAATCCTCCACTCAACCTTCAAG
AAACTCAAGTCACTATAGAAGAAATCACTCCTCTTGTCCCCCCACAATCA
GGAGATAAAGGGCAAGAGGATCTCACAAGCTATTTTCTTGAAGCACTTCT
AAAATACATAGTCATTCAGGTAAAAAGTTTAGAATGGAAGAACAAAGAAA
ACCAAGAAAGGGGATTTTCATTTTTGTTTTCACATTTTAAGAAATATTAC
TTGCCTTATATTTTTCCAAACATCTGTAAGGAAAACAGCTTATATCATCC
TATACTTGACATCCCGCAGATGAGACCAAAGCCACATTATGTCGTGATAA
AGAAAGATGCTGAAACCAATGAAGCAATCTATTGTACAAAGGAGCCTTTC
ATTAAGGCTCGTGTTATTGTCATTCGTTGGCTGGTTTCTTTCTGGCTGGA
GCCAAAACCACATACAGGACCTCATATTCCTGGGATGGAAGGTGAAGTCT
TGCCAAAGAATATTCAGAGAGCAGCTGCTAGTTTAGTATCCAGAGAAGAA
AGCAAAAATGATAATGCTGATAAAACAGACAGAACTACAGAACCCGAACA
GTCTCATTCCAATACAAGCACTCTCACGGAGCGAGAACCTAGCTCATCTA
GTCTCTGTAGTATTGATGAAGAACATCTCACAGACATTGAAATAGTTCGC
AGAGTTTTTTCTTCTAAAAGGAGTAATGTAAACTTTGTGACAGAGATATT
TCGTCAGGCATTTTTATTACCAATTTGTGAAGCAGCAGCTATGAGAAAAG
TGGTAAAAGTATATCAAGAATGGATCCAACAAGAGGAAAAACCTTTGTTC
ATGCAAGAGCCTGAAGAAATTGTGATCACTTCTTCAGACCTCCCTTGCAT
TGAAAATGTCACAGACCATGATATTTCAATGGAAGAAGGAGAAAAAAGAG
AAGAGGAAAATGGGACCAATACTGCTGATCATGTTCGAAATTCCAGTTGG
GCAAAAAACGGCTCCTACCAAGGTGCTCTTCATAACGCCTCTGAAGAAGC
CACAGAACAAAACATACGAGCTGGTACCCAGGCAGTTTTGCAGGTGTTTA
TTATAAACTCATCAAATATATTTCTTCTTGAACCTGCAAATGAAATAAAA
AATCTTCTGGATGAACACACAGATATGTGTAAACGCATTCTTAACATTTA
TCGGTACATGGTTGTACAAGTATCAATGGACAAAAAGACTTGGGAACAGA
TGCTGCTTGTGTTGCTCAGAGTCACGGAATCTGTACTGAAGATGCCATCA
CAAGCTTTTCTACAGTTCCAAGGGAAAAAAAATATGACCTTGGCAGGTCG
ACTTGCAGGACCACTTTTCCAGACCCTTATAGTTGCCTGGATCAAAGCAA
ACCTAAATGTGTACATCTCCCGAGAACTTTGGGATGACTTACTGTCAGTA
TTGTCATCGCTGACCTATTGGGAAGAGTTGGCCACTGAGTGGTCACTGAC
TATGGAGACATTAACTAAAGTTTTAGCTAGGAATTTATATAGTTTGGATC
TCAGTGATTTACCATTGGATAAGCTGAGTGAACAGAAACAAAAAAAGCAC
AAAGGGAAAGGAGTTGGACATGAATTTCAGAAAGTTTCAGTTGACAAGTC
ATTTTCTAGAGGATGGAGTCGTGATCAGCCTGGCCAAGCCCCAATGAGAC
AGAGGAGTGCAACAACCACTGGCTCTCCAGGAACCGAAAAGGCGAGGAGT
ATAGTACGGCAAAAAACTGTCGATATTGATGATGCTCAAATACTTCCCCG
CTCAACTAGAGTCAGACATTTTTCACAAAGTGAAGAAACTGGAAATGAAG
TTTTTGGTGCTTTGAATGAGGAGCAGCCATTGCCTCGAAGTAGCAGCACT
TCTGACATCTTGGAACCATTCACTGTTGAACGAGCCAAAGTCAATAAAGA
GGACATGAGCCAAAAACTGCCTCCTCTTAATAGTGATATTGGCGGCAGCA
GTGCTAATGTTCCTGATCTGATGGATGAGTTTATAGCAGAACGACTTCGA
AGTGGTAATGCCTCGACTATGACAAGAAGAGGAAGTAGTCCAGGCAGCCT
GGAAATTCCCAAAGACCTCCCTGATATTCTAAACAAGCAGAACCAGATGC
GCCCTATTGATGACCCAGGTGTGCCCTCAGAATGGACTTCTCCTGCCAGT
GCAGGGAGCAGTGATCTTATCAGCTCAGATAGTCATTCGGATTCTTTCAG
CGCTTTCCAATATGATGGCCGAAAATTTGACAATTTTGGCTTTGGAACCG
ACACTGGGGTTACGTCCTCTGCTGATGTGGATTCAGGTTCTGGCCATCAT
CAGAGTGCTGAAGAGCAGGAAGTGGCTAGTCTAACTACTCTTCATATAGA
TTCTGAAACAAGCAGTCTTAATCAGCAAGCTTTCTCTGCTGAAGTTGCAA
CTATTACTGGTTCAGAAAGTGCTTCCCCAGTCCACTCACCTCTGGGCTCC
AGGTCACAGACTCCTTCCCCTTCTACATTGAATATAGATCACATGGAACA
GAAGGATCTGCAGCTCGACGAGAAGCTCCACCACTCTGTTCTTCAGACAC
CAGATGATCTAGAAATTAGTGAATTTCCATCAGAATGTTGTAGTGTGATG
GCAGGAGGTACTCTGACTGGATGGCATGCTGATGTTGCTACTGTAATGTG
GCGAAGAATGCTAGGCATTTTGGGAGATGTAAATTCAATCATGGATCCTG
AAATACATGCTCAAGTTTTTGATTACCTCTGTGAACTTTGGCAGAATCTA
GCTAAGATTAGAGATAACCTTGGCATTTCAACTGATAACCTGACCTCCCC
TTCTCCACCAGTTTTAATTCCTCCACTGAGAATTCTTACACCTTGGCTTT
TTAAGGCAACCATGTTGACTGATAAATATAAACAAGGTAAATTACATGCA
TATAAACTTATTTGTAATACAATGAAAAGAAGACAAGATGTTTCTCCAAA
TAGAGATTTTCTAACACATTTCTACAATATAATGCACTGTGGATTACTTC
ATATTGACCAGGATATTGTCAATACAATCATCAAACACTGCTCACCTCAA
TTTTTTTCACTTGGTTTGCCTGGTGCCACAATGCTTATTATGGATTTTAT
TGTAGCAGCTGGTAGAGTGGCTTCTTCAGCTTTTCTCAATGCACCAAGAG
TAGAAGCACAAGTTCTTCTGGGATCTTTGGTTTGCTTTCCCAACTTATAT
TGTGAACTGCCTTCTCTTCATCCCAACATTCCTGATGTTGCTGTGTCTCA
GTTTACAGATGTTAAGGAACTTATAATCAAAACTGTATTAAGCTCGGCAA
GAGATGAGCCCTCTGGTCCTGCACGATGTGTAGCACTTTGTAGTTTAGGT
ATTTGGATTTGTGAAGAACTAGTCCATGAGTCTCATCATCCTCAAATTAA
GGAAGCTCTGAATGTGATTTGTGTTTCCTTAAAGTTTACTAATAAAACAG
TAGCCCACGTAGCTTGTAACATGCTTCACATGCTGGTTCATTATGTACCT
AGACTTCAGATTTACCAGCCTGATTCTCCCTTGAAAATTATTCAAATCCT
AATAGCTACCATCACCCATCTTTTACCAAGTACAGAGGCTTCATCTTATG
AAATGGACAAGAGGTTGGTAGTATCTTTACTTCTCTGCCTTCTGGACTGG
ATCATGGCCTTACCTCTAAAGACACTGCTCCAACCATTTCATGCTACGGG
AGCAGAAAGCGATAAAACAGAAAAATCTGTTCTCAATTGCATTTATAAGG
TTTTACATGGGTGTGTTTATGGAGCTCAGTGTTTTAGCAATCCAAGGTAT
TTTCCCATGAGCCTCTCTGATTTGGCATCTGTAGATTATGATCCTTTTAT
GCACTTGGAAAGTCTGAAAGAGCCTGAGCCTCTGCACTCTCCTGACTCAG
AACGATCTTCTAAACTCCAGCCAGTAACAGAAGTGAAAACTCAAATGCAG
CATGGATTAATCTCTATAGCAGCCCGCACTGTTATTACACATCTGGTAAA
TCACCTGGGCCATTATCCAATGAGCGGTGGTCCTGCTATGCTAACAAGTC
AGGTGTGTGAAAATCACGACAATCATTACAGTGAAAGTACTGAACTTTCT
CCTGAACTCTTTGAGAGTCCAAATATCCAGTTCTTTGTGTTAAATAATAC
AACCTTAGTGTCCTGTATCCAGATCAGATCAGAAGAGAATATGCCTGGAG
GAGGTTTATCTGCTGGCCTTGCATCAGCCAATTCAAATGTCAGAATCATA
GTACGTGATCTCTCTGGAAAATATTCATGGGATTCTGCTATACTGTATGG
CCCACCTCCTGTAAGTGGCTTGTCAGAACCTACATCTTTCATGCTTTCAT
TGTCTCACCAAGAGAAGCCAGAAGAGCCTCCGACATCTAATGAATGCTTA
GAAGATATAACCGTAAAAGATGGACTTTCTCTCCAGTTTAAAAGATTTAG
AGAAACTGTACCAACTTGGGATACAATAAGAGATGAAGAAGATGTTCTTG
ATGAGCTCTTGCAGTATTTGGGTGTTACTAGTCCTGAATGCTTACAGAGA
ACTGGAATCTCACTTAATATTCCTGCTCCACAACCTGTGTGCATTTCTGA
AAAACAAGAAAATGATGTTATTAATGCTATCCTTAAGCAACATACAGAAG
AAAAAGAATTTGTTGAGAAGCACTTTAATGACTTAAACATGAAAGCTGTG
GAACAAGATGAACCAATACCTCAAAAACCTCAGTCAGCATTTTATTATTG
CAGATTGCTTCTTAGTATATTGGGAATGAATTCCTGGGACAAACGGAGGA
GCTTTCATCTCCTGAAGAAAAATGAAAAGCTACTTAGAGAACTTAGGAAC
TTGGATTCAAGGCAGTGCCGAGAGACACACAAGATTGCAGTATTTTATGT
TGCTGAAGGACAAGAAGACAAACACTCCATTCTCACCAATACAGGAGGAA
GTCAAGCATATGAAGATTTTGTAGCTGGTCTTGGTTGGGAGGTAAATCTT
ACAAACCATTGTGGTTTTATGGGAGGACTACAAAAAAACAAAAGCACTGG
ATTGACCACTCCATATTTTGCTACCTCTACAGTAGAGGTAATATTTCACG
TGTCAACAAGAATGCCTTCTGATTCTGATGATTCTTTGACCAAAAAATTG
AGACATTTGGGAAATGATGAAGTGCACATTGTTTGGTCAGAGCATACTAG
AGACTACAGGAGAGGAATTATTCCCACAGAATTTGGTGATGTCCTTATTG
TAATATATCCAATGAAAAATCACATGTTCAGTATTCAGATAATGAAAAAA
CCAGAGGTTCCCTTCTTTGGTCCCCTTTTTGATGGTGCTATTGTGAATGG
AAAGGTTCTACCCATTATGGTTAGAGCAACAGCTATAAATGCAAGCCGTG
CTCTGAAATCTCTGATTCCATTGTATCAAAACTTCTATGAGGAGAGAGCA
CGATACCTGCAAACAATTGTCCAGCACCACTTAGAACCAACAACATTTGA
AGATTTTGCAGCACAGGTTTTTTCTCCAGCTCCCTACCACCATTTACCAT
CTGATGCCGGCTCCTACCCAGAGATTCTACCCAGTGAAACTCCCACAGCA
ACGCAGGTAGATGGGGCTGACCTGGCCTCTCCAATGTCTCCTCGAACTAG
CAAAAGCCGCATGTCCATGAAGCTGCGTCGTTCCTCTGGCTCAGCCAATA
AATCCTAAGCGGCCGC

Amino Acid Sequence

Amino acid sequence of expressed protein. MYPYDVPDYAGSMFSKKPHGDVKKSTQKVLDTKKDALTRLKHLRIVIENA
ESIDLKQFFDQHFSHIYYVFFENFVTIEASLKQKGHKSQREELDAILFIF
EKILQLLPERIHQRWQFHSIGLILKKLLHTGNSLKIRREGVRLFLLWLQA
LQNNCSKEQLWMFSCLIPGFSAPQSEHGPRTLDNLINPPLNLQETQVTIE
EITPLVPPQSGDKGQEDLTSYFLEALLKYIVIQVKSLEWKNKENQERGFS
FLFSHFKKYYLPYIFPNICKENSLYHPILDIPQMRPKPHYVVIKKDAETN
EAIYCTKEPFIKARVIVIRWLVSFWLEPKPHTGPHIPGMEGEVLPKNIQR
AAASLVSREESKNDNADKTDRTTEPEQSHSNTSTLTEREPSSSSLCSIDE
EHLTDIEIVRRVFSSKRSNVNFVTEIFRQAFLLPICEAAAMRKVVKVYQE
WIQQEEKPLFMQEPEEIVITSSDLPCIENVTDHDISMEEGEKREEENGTN
TADHVRNSSWAKNGSYQGALHNASEEATEQNIRAGTQAVLQVFIINSSNI
FLLEPANEIKNLLDEHTDMCKRILNIYRYMVVQVSMDKKTWEQMLLVLLR
VTESVLKMPSQAFLQFQGKKNMTLAGRLAGPLFQTLIVAWIKANLNVYIS
RELWDDLLSVLSSLTYWEELATEWSLTMETLTKVLARNLYSLDLSDLPLD
KLSEQKQKKHKGKGVGHEFQKVSVDKSFSRGWSRDQPGQAPMRQRSATTT
GSPGTEKARSIVRQKTVDIDDAQILPRSTRVRHFSQSEETGNEVFGALNE
EQPLPRSSSTSDILEPFTVERAKVNKEDMSQKLPPLNSDIGGSSANVPDL
MDEFIAERLRSGNASTMTRRGSSPGSLEIPKDLPDILNKQNQMRPIDDPG
VPSEWTSPASAGSSDLISSDSHSDSFSAFQYDGRKFDNFGFGTDTGVTSS
ADVDSGSGHHQSAEEQEVASLTTLHIDSETSSLNQQAFSAEVATITGSES
ASPVHSPLGSRSQTPSPSTLNIDHMEQKDLQLDEKLHHSVLQTPDDLEIS
EFPSECCSVMAGGTLTGWHADVATVMWRRMLGILGDVNSIMDPEIHAQVF
DYLCELWQNLAKIRDNLGISTDNLTSPSPPVLIPPLRILTPWLFKATMLT
DKYKQGKLHAYKLICNTMKRRQDVSPNRDFLTHFYNIMHCGLLHIDQDIV
NTIIKHCSPQFFSLGLPGATMLIMDFIVAAGRVASSAFLNAPRVEAQVLL
GSLVCFPNLYCELPSLHPNIPDVAVSQFTDVKELIIKTVLSSARDEPSGP
ARCVALCSLGIWICEELVHESHHPQIKEALNVICVSLKFTNKTVAHVACN
MLHMLVHYVPRLQIYQPDSPLKIIQILIATITHLLPSTEASSYEMDKRLV
VSLLLCLLDWIMALPLKTLLQPFHATGAESDKTEKSVLNCIYKVLHGCVY
GAQCFSNPRYFPMSLSDLASVDYDPFMHLESLKEPEPLHSPDSERSSKLQ
PVTEVKTQMQHGLISIAARTVITHLVNHLGHYPMSGGPAMLTSQVCENHD
NHYSESTELSPELFESPNIQFFVLNNTTLVSCIQIRSEENMPGGGLSAGL
ASANSNVRIIVRDLSGKYSWDSAILYGPPPVSGLSEPTSFMLSLSHQEKP
EEPPTSNECLEDITVKDGLSLQFKRFRETVPTWDTIRDEEDVLDELLQYL
GVTSPECLQRTGISLNIPAPQPVCISEKQENDVINAILKQHTEEKEFVEK
HFNDLNMKAVEQDEPIPQKPQSAFYYCRLLLSILGMNSWDKRRSFHLLKK
NEKLLRELRNLDSRQCRETHKIAVFYVAEGQEDKHSILTNTGGSQAYEDF
VAGLGWEVNLTNHCGFMGGLQKNKSTGLTTPYFATSTVEVIFHVSTRMPS
DSDDSLTKKLRHLGNDEVHIVWSEHTRDYRRGIIPTEFGDVLIVIYPMKN
HMFSIQIMKKPEVPFFGPLFDGAIVNGKVLPIMVRATAINASRALKSLIP
LYQNFYEERARYLQTIVQHHLEPTTFEDFAAQVFSPAPYHHLPSDAGSYP
EILPSETPTATQVDGADLASPMSPRTSKSRMSMKLRRSSGSANKS*

• Vector Type: Mammalian
• Antibiotic: Amp
• Full Snapgene Map: pcDNA5-FRT/TO-HA GRIPE224.33 KB Snapgene files can be opened using software available here: www.snapgene.com
• DU Number: DU31362
• Unit Source: Protein Phosphorylation and Ubiquitylation Unit
• Location: PPU
• MTA: Dundee
• Notes: Internal BglII killed with silent change a612g; Additional silent changes: t783c, t2217c, t3531c, t4398c
• Low Temp: No