cDNA Clone GRB2

• Plasmid: pEBG6P-1 GRB2 M1-E152
• Parent Plasmid: pEBG6P
• Expressed: GST-GRB2 M1-E152
• Genbank: NM_002086.4 GI:156071491
• Tag: GST
• Species: Human

Sequence of Insert

GGATCCATGGAAGCCATCGCCAAATATGACTTCAAAGCTACTGCAGACGA
CGAGCTGAGCTTCAAAAGGGGGGACATCCTCAAGGTTTTGAACGAAGAAT
GTGATCAGAACTGGTACAAGGCAGAGCTTAATGGAAAAGACGGCTTCATT
CCCAAGAACTACATAGAAATGAAACCACATCCGTGGTTTTTTGGCAAAAT
CCCCAGAGCCAAGGCAGAAGAAATGCTTAGCAAACAGCGGCACGATGGGG
CCTTTCTTATCCGAGAGAGTGAGAGCGCTCCTGGGGACTTCTCCCTCTCT
GTCAAGTTTGGAAACGATGTGCAGCACTTCAAGGTGCTCCGAGATGGAGC
CGGGAAGTACTTCCTCTGGGTGGTGAAGTTCAATTCTTTGAATGAGCTGG
TGGATTATCACAGATCTACATCTGTCTCCAGAAACCAGCAGATATTCCTG
CGGGACATAGAATAAGCGGCCGC

Amino Acid Sequence

MAPILGYWKIKGLVQPTRLLLEYLEEKYEEHLYERDEGDKWRNKKFELGL
EFPNLPYYIDGDVKLTQSMAIIRYIADKHNMLGGCPKERAEISMLEGAVL
DIRYGVSRIAYSKDFETLKVDFLSKLPEMLKMFEDRLCHKTYLNGDHVTH
PDFMLYDALDVVLYMDPMCLDAFPKLVCFKKRIEAIPQIDKYLKSSKYIA
WPLQGWQATFGGGDHPPKSDLVPRGSLEVLFQGPLGSMEAIAKYDFKATA
DDELSFKRGDILKVLNEECDQNWYKAELNGKDGFIPKNYIEMKPHPWFFG
KIPRAKAEEMLSKQRHDGAFLIRESESAPGDFSLSVKFGNDVQHFKVLRD
GAGKYFLWVVKFNSLNELVDYHRSTSVSRNQQIFLRDIE*

• Vector Type: Mammalian
• Antibiotic: Amp
• DU Number: DU31354
• Unit Source: Protein Phosphorylation and Ubiquitylation Unit
• Location: PPU
• MTA: Dundee
• Notes: Mammalian expression plasmid to express with N-terminal GST (contains preScission protease site)