cDNA Clone ZNRF2

• Plasmid: pcDNA5-FRT/TO-FLAG ZNRF2 C199A
• Parent Plasmid: pcDNA5D FRT/TO FLAG
• Expressed: FLAG ZNRF2 C199A
• Genbank: NM_147128.3 GI:40316919
• Tag: FLAG
• Species: Human

Sequence of Insert

GGATCCATGGGCGCCAAACAGAGCGGCCCGGCCGCCGCTAACGGCCGCAC
GCGCGCGTACTCGGGCTCGGATCTACCTTCCAGTAGCAGCGGAGGCGCCA
ATGGGACCGCGGGCGGCGGCGGGGGCGCTCGGGCCGCCGCCGCGGGGAGG
TTCCCGGCTCAGGTGCCCAGCGCGCACCAGCCCAGCGCCTCCGGCGGCGC
CGCGGCGGCCGCGGCGGCCCCGGCAGCCCCGGCGGCCCCGCGCAGCCGCT
CCCTCGGCGGGGCCGTGGGGAGCGTGGCGTCGGGGGCCCGCGCGGCGCAG
TCCCCCTTCAGCATCCCGAACAGCAGCAGCGGCCCGTACGGCTCGCAGGA
CTCGGTGCACAGCAGCCCTGAGGACGGCGGCGGCGGCCGGGACCGGCCGG
TGGGCGGGAGCCCCGGCGGGCCGCGCCTGGTGATCGGCTCCTTACCAGCT
CACCTCTCGCCGCACATGTTTGGAGGATTTAAGTGCCCTGTATGCTCAAA
ATTTGTATCCTCAGATGAAATGGATTTGCATCTTGTAATGTGTTTAACAA
AGCCACGAATAACCTATAATGAGGATGTACTGAGTAAAGATGCTGGGGAA
GCTGCAATATGCCTTGAAGAATTGCAGCAGGGAGATACTATAGCACGACT
GCCTTGTCTATGCATATATCATAAAGGCTGCATAGATGAATGGTTTGAAG
TAAATAGATCTTGCCCTGAGCACCCTTCAGATTAAGGATCC

Amino Acid Sequence

MDYKDDDDKGSMGAKQSGPAAANGRTRAYSGSDLPSSSSGGANGTAGGGG
GARAAAAGRFPAQVPSAHQPSASGGAAAAAAAPAAPAAPRSRSLGGAVGS
VASGARAAQSPFSIPNSSSGPYGSQDSVHSSPEDGGGGRDRPVGGSPGGP
RLVIGSLPAHLSPHMFGGFKCPVCSKFVSSDEMDLHLVMCLTKPRITYNE
DVLSKDAGEAAICLEELQQGDTIARLPCLCIYHKGCIDEWFEVNRSCPEH
PSD*

• Vector Type: Mammalian
• Antibiotic: Amp
• DU Number: DU31332
• Unit Source: Protein Phosphorylation and Ubiquitylation Unit
• Location: PPU
• MTA: Dundee
• Notes: All ZNRF2 plasmids MUST be grown at 30 degrees C or less to prevent recombination.