cDNA Clone GRB2

• Plasmid: pEBG6P-1 GRB2 H58-end
• Parent Plasmid: pEBG6P
• Expressed: GST-GRB2 H58-end
• Genbank: NM_002086.4 GI:156071491
• Tag: GST
• Species: Human

Sequence of Insert

GGATCCCATCCGTGGTTTTTTGGCAAAATCCCCAGAGCCAAGGCAGAAGA
AATGCTTAGCAAACAGCGGCACGATGGGGCCTTTCTTATCCGAGAGAGTG
AGAGCGCTCCTGGGGACTTCTCCCTCTCTGTCAAGTTTGGAAACGATGTG
CAGCACTTCAAGGTGCTCCGAGATGGAGCCGGGAAGTACTTCCTCTGGGT
GGTGAAGTTCAATTCTTTGAATGAGCTGGTGGATTATCACAGATCTACAT
CTGTCTCCAGAAACCAGCAGATATTCCTGCGGGACATAGAACAGGTGCCA
CAGCAGCCGACATACGTCCAGGCCCTCTTTGACTTTGATCCCCAGGAGGA
TGGAGAGCTGGGCTTCCGCCGGGGAGATTTTATCCATGTCATGGATAACT
CAGACCCCAACTGGTGGAAAGGAGCTTGCCACGGGCAGACCGGCATGTTT
CCCCGCAATTATGTCACCCCCGTGAACCGGAACGTCTAAGCGGCCGC

Amino Acid Sequence

MAPILGYWKIKGLVQPTRLLLEYLEEKYEEHLYERDEGDKWRNKKFELGL
EFPNLPYYIDGDVKLTQSMAIIRYIADKHNMLGGCPKERAEISMLEGAVL
DIRYGVSRIAYSKDFETLKVDFLSKLPEMLKMFEDRLCHKTYLNGDHVTH
PDFMLYDALDVVLYMDPMCLDAFPKLVCFKKRIEAIPQIDKYLKSSKYIA
WPLQGWQATFGGGDHPPKSDLVPRGSLEVLFQGPLGSHPWFFGKIPRAKA
EEMLSKQRHDGAFLIRESESAPGDFSLSVKFGNDVQHFKVLRDGAGKYFL
WVVKFNSLNELVDYHRSTSVSRNQQIFLRDIEQVPQQPTYVQALFDFDPQ
EDGELGFRRGDFIHVMDNSDPNWWKGACHGQTGMFPRNYVTPVNRNV*

• Vector Type: Mammalian
• Antibiotic: Amp
• DU Number: DU31327
• Unit Source: Protein Phosphorylation and Ubiquitylation Unit
• Location: PPU
• MTA: Dundee
• Notes: Mammalian expression plasmid to express GRB2 H58-end with N-terminal GST (contains preScission protease site); ; Estimated MW of expressed protein : 45995 Daltons