cDNA Clone BRCA1

• Plasmid: pcDNA5-FRT/TO-GFP BRCA1
• Parent Plasmid: pcDNA5 FRT/TO GFP
• Expressed: GFP BRCA1
• Genbank: AAA73985.1
• Tag: GFP
• Species: Human

Sequence of Insert

GGATCCATGGATTTATCTGCTCTTCGCGTTGAAGAAGTACAAAATGTCAT
TAATGCTATGCAGAAAATCTTAGAGTGTCCCATCTGTCTGGAGTTGATCA
AGGAACCTGTCTCCACAAAGTGTGACCACATATTTTGCAAATTTTGCATG
CTGAAACTTCTCAACCAGAAGAAAGGGCCTTCACAGTGTCCTTTATGTAA
GAATGATATAACCAAAAGGAGCCTACAAGAAAGTACGAGATTTAGTCAAC
TTGTTGAAGAGCTATTGAAAATCATTTGTGCTTTTCAGCTTGACACAGGT
TTGGAGTATGCAAACAGCTATAATTTTGCAAAAAAGGAAAATAACTCTCC
TGAACATCTAAAAGATGAAGTTTCTATCATCCAAAGTATGGGCTACAGAA
ACCGTGCCAAAAGACTTCTACAGAGTGAACCCGAAAATCCTTCCTTGCAG
GAAACCAGTCTCAGTGTCCAACTCTCTAACCTTGGAACTGTGAGAACTCT
GAGGACAAAGCAGCGGATACAACCTCAAAAGACGTCTGTCTACATTGAAT
TGGGATCTGATTCTTCTGAAGATACCGTTAATAAGGCAACTTATTGCAGT
GTGGGAGATCAAGAATTGTTACAAATCACCCCTCAAGGAACCAGGGATGA
AATCAGTTTGGATTCTGCAAAAAAGGCTGCTTGTGAATTTTCTGAGACGG
ATGTAACAAATACTGAACATCATCAACCCAGTAATAATGATTTGAACACC
ACTGAGAAGCGTGCAGCTGAGAGGCATCCAGAAAAGTATCAGGGTAGTTC
TGTTTCAAACTTGCATGTGGAGCCATGTGGCACAAATACTCATGCCAGCT
CATTACAGCATGAGAACAGCAGTTTATTACTCACTAAAGACAGAATGAAT
GTAGAAAAGGCTGAATTCTGTAATAAAAGCAAACAGCCTGGCTTAGCAAG
GAGCCAACATAACAGATGGGCTGGAAGTAAGGAAACATGTAATGATAGGC
GGACTCCCAGCACAGAAAAAAAGGTAGATCTGAATGCTGATCCCCTGTGT
GAGAGAAAAGAATGGAATAAGCAGAAACTGCCATGCTCAGAGAATCCTAG
AGATACTGAAGATGTTCCTTGGATAACACTAAATAGCAGCATTCAGAAAG
TTAATGAGTGGTTTTCCAGAAGTGATGAACTGTTAGGTTCTGATGACTCA
CATGATGGGGAGTCTGAATCAAATGCCAAAGTAGCTGATGTATTGGACGT
TCTAAATGAGGTAGATGAATATTCTGGTTCTTCAGAGAAAATAGACTTAC
TGGCCAGTGATCCTCATGAGGCTTTAATATGTAAAAGTGAAAGAGTTCAC
TCCAAATCAGTAGAGAGTAATATTGAAGACAAAATATTTGGGAAAACCTA
TCGGAAGAAGGCAAGCCTCCCCAACTTAAGCCATGTAACTGAAAATCTAA
TTATAGGAGCATTTGTTACTGAGCCACAGATAATACAAGAGCGTCCCCTC
ACAAATAAATTAAAGCGTAAAAGGAGACCTACATCAGGCCTTCATCCTGA
GGATTTTATCAAGAAAGCAGATTTGGCAGTTCAAAAGACTCCTGAAATGA
TAAATCAGGGAACTAACCAAACGGAGCAGAATGGTCAAGTGATGAATATT
ACTAATAGTGGTCATGAGAATAAAACAAAAGGTGATTCTATTCAGAATGA
GAAAAATCCTAACCCAATAGAATCACTCGAAAAAGAATCTGCTTTCAAAA
CGAAAGCTGAACCTATAAGCAGCAGTATAAGCAATATGGAACTCGAATTA
AATATCCACAATTCAAAAGCACCTAAAAAGAATAGGCTGAGGAGGAAGTC
TTCTACCAGGCATATTCATGCGCTTGAACTAGTAGTCAGTAGAAATCTAA
GCCCACCTAATTGTACTGAATTGCAAATTGATAGTTGTTCTAGCAGTGAA
GAGATAAAGAAAAAAAAGTACAACCAAATGCCAGTCAGGCACAGCAGAAA
CCTACAACTCATGGAAGGTAAAGAACCTGCAACTGGAGCCAAGAAGAGTA
ACAAGCCAAATGAACAGACAAGTAAAAGACATGACAGTGATACTTTCCCA
GAGCTGAAGTTAACAAATGCACCTGGTTCTTTTACTAAGTGTTCAAATAC
CAGTGAACTTAAAGAATTTGTCAATCCTAGCCTTCCAAGAGAAGAAAAAG
AAGAGAAACTAGAAACAGTTAAAGTGTCTAATAATGCTGAAGACCCCAAA
GATCTCATGTTAAGTGGAGAAAGGGTTTTGCAAACTGAAAGATCTGTAGA
GAGTAGCAGTATTTCACTGGTACCTGGTACTGATTATGGCACTCAGGAAA
GTATCTCGTTACTGGAAGTTAGCACTCTAGGGAAGGCAAAAACAGAACCA
AATAAATGTGTGAGTCAGTGTGCAGCATTTGAAAACCCCAAGGGACTAAT
TCATGGTTGTTCCAAAGATAATAGAAATGACACAGAAGGCTTTAAGTATC
CATTGGGACATGAAGTTAACCACAGTCGGGAAACAAGCATAGAAATGGAA
GAAAGTGAACTCGATGCTCAGTATTTGCAGAATACATTCAAGGTTTCAAA
GCGCCAGTCATTTGCTCTGTTTTCAAATCCAGGAAATGCAGAAGAGGAAT
GTGCAACATTCTCTGCCCACTCTGGGTCCTTAAAGAAACAAAGTCCAAAA
GTCACTTTTGAATGTGAACAAAAGGAAGAAAATCAAGGAAAGAATGAGTC
TAATATCAAGCCTGTACAGACAGTTAATATCACTGCAGGCTTTCCTGTGG
TTGGTCAGAAAGATAAGCCAGTTGATAATGCCAAATGTAGTATCAAAGGA
GGCTCTAGGTTTTGTCTATCATCTCAGTTCAGAGGCAACGAAACTGGACT
CATTACTCCAAATAAACATGGACTTTTACAAAACCCATATCGTATACCAC
CACTTTTTCCCATCAAGTCATTTGTTAAAACTAAATGTAAGAAAAATCTG
CTAGAGGAAAACTTTGAGGAACATTCAATGTCACCTGAAAGAGAAATGGG
AAATGAGAACATTCCAAGTACAGTGAGCACAATTAGCCGTAATAACATTA
GAGAAAATGTTTTTAAAGGAGCCAGCTCAAGCAATATTAATGAAGTAGGT
TCCAGTACTAATGAAGTGGGCTCCAGTATTAATGAAATAGGTTCCAGTGA
TGAAAACATTCAAGCAGAACTAGGTAGAAACAGAGGGCCAAAATTGAATG
CTATGCTTAGATTAGGGGTTTTGCAACCTGAGGTCTATAAACAAAGTCTT
CCTGGAAGTAATTGTAAGCATCCTGAAATAAAAAAGCAAGAATATGAAGA
AGTAGTTCAGACTGTTAATACAGATTTCTCTCCATATCTGATTTCAGATA
ACTTAGAACAGCCTATGGGAAGTAGTCATGCATCTCAGGTTTGTTCTGAG
ACACCTGATGACCTGTTAGATGATGGTGAAATAAAGGAAGATACTAGTTT
TGCTGAAAATGACATTAAGGAAAGTTCTGCTGTTTTTAGCAAAAGCGTCC
AGAGAGGAGAGCTTAGCAGGAGTCCTAGCCCTTTCACCCATACACATTTG
GCTCAGGGTTACCGAAGAGGGGCCAAGAAATTAGAGTCCTCAGAAGAGAA
CTTATCTAGTGAGGATGAAGAGCTTCCCTGCTTCCAACACTTGTTATTTG
GTAAAGTAAACAATATACCTTCTCAGTCTACTAGGCATAGCACCGTTGCT
ACCGAGTGTCTGTCTAAGAACACAGAGGAGAATTTATTATCATTGAAGAA
TAGCTTAAATGACTGCAGTAACCAGGTAATATTGGCAAAGGCATCTCAGG
AACATCACCTTAGTGAGGAAACAAAATGTTCTGCTAGCTTGTTTTCTTCA
CAGTGCAGTGAATTGGAAGACTTGACTGCAAATACAAACACCCAAGACCC
TTTCTTGATTGGTTCTTCCAAACAAATGAGGCATCAGTCTGAAAGCCAGG
GAGTTGGTCTGAGTGACAAGGAATTGGTTTCAGATGATGAAGAAAGAGGA
ACGGGCTTGGAAGAAAATAATCAAGAAGAGCAAAGCATGGATTCAAACTT
AGGTGAAGCAGCATCTGGGTGTGAGAGTGAAACAAGCGTCTCTGAAGACT
GCTCAGGGCTATCCTCTCAGAGTGACATTTTAACCACTCAGCAGAGGGAT
ACCATGCAACATAACCTGATAAAGCTCCAGCAGGAAATGGCTGAACTAGA
AGCTGTGTTAGAACAGCATGGGAGCCAGCCTTCTAACAGCTACCCTTCCA
TCATAAGTGACTCCTCTGCCCTTGAGGACCTGCGAAATCCAGAACAAAGC
ACATCAGAAAAAGATTCGCATATACATGGCCAAAGGGACAACTCCATGTT
TTCTAAAAGGCCTAGAGAACATATATCAGTATTAACTTCACAGAAAAGTA
GTGAATACCCTATAAGCCAGAATCCAGAAGGCCTTTCTGCTGACAAGTTT
GAGGTGTCTGCAGATAGTTCTACCAGTAAAAATAAAGAACCAGGAGTGGA
AAGGTCATCCCCTTCTAAATGCCCATCATTAGATGATAGGTGGTACATGC
ACAGTTGCTCTGGGAGTCTTCAGAATAGAAACTACCCATCTCAAGAGGAG
CTCATTAAGGTTGTTGATGTGGAGGAGCAACAGCTGGAAGAGTCTGGGCC
ACACGATTTGACGGAAACATCTTACTTGCCAAGGCAAGATCTAGAGGGAA
CCCCTTACCTGGAATCTGGAATCAGCCTCTTCTCTGATGACCCTGAATCT
GATCCTTCTGAAGACAGAGCCCCAGAGTCAGCTCGTGTTGGCAACATACC
ATCTTCAACCTCTGCATTGAAATTTCCCCAATTGAAAGTTGCAGAATCTG
CCCAGGGTCCAGCTGCTGCTCATACTACTGATACTGCTGGGTATAATGCA
ATGGAAGAAAGTGTGAGCAGGGAGAAGCCAGAATTGACAGCTTCAACAGA
AAGGGTCAACAAAAGAATGTCCATGGTGGTGTCTGGCCTGACCCCAGAAG
AATTTATGCTCGTGTACAAGTTTGCCAGAAAACACCACATCACTTTAACT
AATCTAATTACTGAAGAGACTACTCATGTTGTTATGAAAACAGATGCTGA
GTTTGTGTGTGAACGGACACTGAAATATTTTCTAGGAATTGCGGGAGGAA
AATGGGTAGTTAGCTATTTCTGGGTGACCCAGTCTATTAAAGAAAGAAAA
ATGCTGAATGAGCATGATTTTGAAGTCAGAGGAGATGTGGTCAATGGAAG
AAACCACCAAGGTCCAAAGCGAGCAAGAGAATCCCAGGACAGAAAGATCT
TCAGGGGGCTAGAAATCTGTTGCTATGGGCCCTTCACCAACATGCCCACA
GATCAACTGGAATGGATGGTACAGCTGTGTGGTGCTTCTGTGGTGAAGGA
GCTTTCATCATTCACCCTTGGCACAGGTGTCCACCCAATTGTGGTTGTGC
AGCCAGATGCCTGGACAGAGGACAATGGCTTCCATGCAATTGGGCAGATG
TGTGAGGCACCTGTGGTGACCCGAGAGTGGGTGTTGGACAGTGTAGCACT
CTACCAGTGCCAGGAGCTGGACACCTACCTGATACCCCAGATCCCCCACA
GCCACTACTGAGCGGCCGC

Amino Acid Sequence

MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTLKFICT
TGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIF
FKDDGNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHN
VYIMADKQKNGIKVNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNH
YLSTQSALSKDPNEKRDHMVLLEFVTAAGITLGMDELYKSGLGSMDLSAL
RVEEVQNVINAMQKILECPICLELIKEPVSTKCDHIFCKFCMLKLLNQKK
GPSQCPLCKNDITKRSLQESTRFSQLVEELLKIICAFQLDTGLEYANSYN
FAKKENNSPEHLKDEVSIIQSMGYRNRAKRLLQSEPENPSLQETSLSVQL
SNLGTVRTLRTKQRIQPQKTSVYIELGSDSSEDTVNKATYCSVGDQELLQ
ITPQGTRDEISLDSAKKAACEFSETDVTNTEHHQPSNNDLNTTEKRAAER
HPEKYQGSSVSNLHVEPCGTNTHASSLQHENSSLLLTKDRMNVEKAEFCN
KSKQPGLARSQHNRWAGSKETCNDRRTPSTEKKVDLNADPLCERKEWNKQ
KLPCSENPRDTEDVPWITLNSSIQKVNEWFSRSDELLGSDDSHDGESESN
AKVADVLDVLNEVDEYSGSSEKIDLLASDPHEALICKSERVHSKSVESNI
EDKIFGKTYRKKASLPNLSHVTENLIIGAFVTEPQIIQERPLTNKLKRKR
RPTSGLHPEDFIKKADLAVQKTPEMINQGTNQTEQNGQVMNITNSGHENK
TKGDSIQNEKNPNPIESLEKESAFKTKAEPISSSISNMELELNIHNSKAP
KKNRLRRKSSTRHIHALELVVSRNLSPPNCTELQIDSCSSSEEIKKKKYN
QMPVRHSRNLQLMEGKEPATGAKKSNKPNEQTSKRHDSDTFPELKLTNAP
GSFTKCSNTSELKEFVNPSLPREEKEEKLETVKVSNNAEDPKDLMLSGER
VLQTERSVESSSISLVPGTDYGTQESISLLEVSTLGKAKTEPNKCVSQCA
AFENPKGLIHGCSKDNRNDTEGFKYPLGHEVNHSRETSIEMEESELDAQY
LQNTFKVSKRQSFALFSNPGNAEEECATFSAHSGSLKKQSPKVTFECEQK
EENQGKNESNIKPVQTVNITAGFPVVGQKDKPVDNAKCSIKGGSRFCLSS
QFRGNETGLITPNKHGLLQNPYRIPPLFPIKSFVKTKCKKNLLEENFEEH
SMSPEREMGNENIPSTVSTISRNNIRENVFKGASSSNINEVGSSTNEVGS
SINEIGSSDENIQAELGRNRGPKLNAMLRLGVLQPEVYKQSLPGSNCKHP
EIKKQEYEEVVQTVNTDFSPYLISDNLEQPMGSSHASQVCSETPDDLLDD
GEIKEDTSFAENDIKESSAVFSKSVQRGELSRSPSPFTHTHLAQGYRRGA
KKLESSEENLSSEDEELPCFQHLLFGKVNNIPSQSTRHSTVATECLSKNT
EENLLSLKNSLNDCSNQVILAKASQEHHLSEETKCSASLFSSQCSELEDL
TANTNTQDPFLIGSSKQMRHQSESQGVGLSDKELVSDDEERGTGLEENNQ
EEQSMDSNLGEAASGCESETSVSEDCSGLSSQSDILTTQQRDTMQHNLIK
LQQEMAELEAVLEQHGSQPSNSYPSIISDSSALEDLRNPEQSTSEKDSHI
HGQRDNSMFSKRPREHISVLTSQKSSEYPISQNPEGLSADKFEVSADSST
SKNKEPGVERSSPSKCPSLDDRWYMHSCSGSLQNRNYPSQEELIKVVDVE
EQQLEESGPHDLTETSYLPRQDLEGTPYLESGISLFSDDPESDPSEDRAP
ESARVGNIPSSTSALKFPQLKVAESAQGPAAAHTTDTAGYNAMEESVSRE
KPELTASTERVNKRMSMVVSGLTPEEFMLVYKFARKHHITLTNLITEETT
HVVMKTDAEFVCERTLKYFLGIAGGKWVVSYFWVTQSIKERKMLNEHDFE
VRGDVVNGRNHQGPKRARESQDRKIFRGLEICCYGPFTNMPTDQLEWMVQ
LCGASVVKELSSFTLGTGVHPIVVVQPDAWTEDNGFHAIGQMCEAPVVTR
EWVLDSVALYQCQELDTYLIPQIPHSHY*

• Vector Type: Mammalian
• Antibiotic: Amp
• Full Snapgene Map: pcDNA5-FRT/TO-GFP BRCA1129.93 KBSnapgene files can be opened using software available here: www.snapgene.com
• DU Number: DU31297
• Unit Source: Protein Phosphorylation and Ubiquitylation Unit
• Location: PPU
• MTA: Dundee
• Low Temp: No