cDNA Clone MLK2

• Plasmid: pGEX6P-MLK2 72-377
• Parent Plasmid: pGEX6P1
• Expressed: GST-MLK2 72-377
• Genbank: NM_002446.3 GI:221316714
• Tag: GST
• Species: Human

Sequence of Insert

GGATCCCCCAGCAACTACGTGGCCCCCGGCGCCCCCGCTGCACCCGCGGG
CCTCCAGCTGCCCCAGGAGATCCCCTTCCACGAGCTGCAGCTAGAGGAGA
TCATCGGTGTGGGGGGCTTTGGCAAGGTCTATCGGGCCCTGTGGCGTGGC
GAGGAGGTGGCAGTCAAGGCCGCCCGGCTGGACCCTGAGAAGGACCCGGC
AGTGACAGCGGAGCAGGTGTGCCAGGAAGCCCGGCTCTTTGGAGCCCTGC
AGCACCCCAACATAATTGCCCTTAGGGGCGCCTGCCTCAACCCCCCACAC
CTCTGCCTAGTGATGGAGTATGCCCGGGGTGGTGCACTGAGCAGGGTGCT
GGCAGGTCGCCGGGTGCCACCTCACGTGCTGGTCAACTGGGCTGTGCAGG
TGGCCCGGGGCATGAACTACCTACACAATGATGCCCCTGTGCCCATCATC
CACCGGGACCTCAAGTCCATCAACATCCTGATCCTGGAGGCCATCGAGAA
CCACAACCTCGCAGACACGGTGCTCAAGATCACGGACTTCGGCCTCGCCC
GCGAGTGGCACAAGACCACCAAGATGAGCGCTGCGGGGACCTACGCCTGG
ATGGCGCCGGAGGTTATCCGTCTCTCCCTCTTCTCCAAAAGCAGTGATGT
CTGGAGCTTCGGGGTGCTGCTGTGGGAGCTGCTGACGGGGGAGGTCCCCT
ACCGTGAGATCGACGCCTTGGCCGTGGCGTATGGCGTGGCTATGAATAAG
CTGACGCTGCCCATTCCCTCCACGTGCCCCGAGCCCTTTGCCCGCCTCCT
GGAGGAATGCTGGGACCCAGACCCCCACGGGCGGCCAGATTTCGGTAGCA
TCTTGAAGCGGCTTGAAGTCATCGAACAGTCAGCCCTGTTCCAGATGCCA
CTGGAGTCCTTCCACTCGCTGCAGTAAGCGGCCGC

Amino Acid Sequence

MSPILGYWKIKGLVQPTRLLLEYLEEKYEEHLYERDEGDKWRNKKFELGL
EFPNLPYYIDGDVKLTQSMAIIRYIADKHNMLGGCPKERAEISMLEGAVL
DIRYGVSRIAYSKDFETLKVDFLSKLPEMLKMFEDRLCHKTYLNGDHVTH
PDFMLYDALDVVLYMDPMCLDAFPKLVCFKKRIEAIPQIDKYLKSSKYIA
WPLQGWQATFGGGDHPPKSDLEVLFQGPLGSPSNYVAPGAPAAPAGLQLP
QEIPFHELQLEEIIGVGGFGKVYRALWRGEEVAVKAARLDPEKDPAVTAE
QVCQEARLFGALQHPNIIALRGACLNPPHLCLVMEYARGGALSRVLAGRR
VPPHVLVNWAVQVARGMNYLHNDAPVPIIHRDLKSINILILEAIENHNLA
DTVLKITDFGLAREWHKTTKMSAAGTYAWMAPEVIRLSLFSKSSDVWSFG
VLLWELLTGEVPYREIDALAVAYGVAMNKLTLPIPSTCPEPFARLLEECW
DPDPHGRPDFGSILKRLEVIEQSALFQMPLESFHSLQ*

• Vector Type: Bacterial
• Antibiotic: Amp
• DU Number: DU31283
• Unit Source: Protein Phosphorylation and Ubiquitylation Unit
• Location: PPU
• MTA: Dundee
• Notes: Bacterial construct to express GST N terminal fusion ; ; Estimated MW of expressed protein : 60644 Daltons