cDNA Clone GRB2

• Plasmid: pGEX6P-GRB2
• Parent Plasmid: pGEX6P1
• Expressed: GST GRB2
• Genbank: NM_002086.4 GI:156071491
• Tag: GST
• Species: Human

Sequence of Insert

GGATCCATGGAAGCCATCGCCAAATATGACTTCAAAGCTACTGCAGACGA
CGAGCTGAGCTTCAAAAGGGGGGACATCCTCAAGGTTTTGAACGAAGAAT
GTGATCAGAACTGGTACAAGGCAGAGCTTAATGGAAAAGACGGCTTCATT
CCCAAGAACTACATAGAAATGAAACCACATCCGTGGTTTTTTGGCAAAAT
CCCCAGAGCCAAGGCAGAAGAAATGCTTAGCAAACAGCGGCACGATGGGG
CCTTTCTTATCCGAGAGAGTGAGAGCGCTCCTGGGGACTTCTCCCTCTCT
GTCAAGTTTGGAAACGATGTGCAGCACTTCAAGGTGCTCCGAGATGGAGC
CGGGAAGTACTTCCTCTGGGTGGTGAAGTTCAATTCTTTGAATGAGCTGG
TGGATTATCACAGATCTACATCTGTCTCCAGAAACCAGCAGATATTCCTG
CGGGACATAGAACAGGTGCCACAGCAGCCGACATACGTCCAGGCCCTCTT
TGACTTTGATCCCCAGGAGGATGGAGAGCTGGGCTTCCGCCGGGGAGATT
TTATCCATGTCATGGATAACTCAGACCCCAACTGGTGGAAAGGAGCTTGC
CACGGGCAGACCGGCATGTTTCCCCGCAATTATGTCACCCCCGTGAACCG
GAACGTCTAAGCGGCCGC

Amino Acid Sequence

MSPILGYWKIKGLVQPTRLLLEYLEEKYEEHLYERDEGDKWRNKKFELGL
EFPNLPYYIDGDVKLTQSMAIIRYIADKHNMLGGCPKERAEISMLEGAVL
DIRYGVSRIAYSKDFETLKVDFLSKLPEMLKMFEDRLCHKTYLNGDHVTH
PDFMLYDALDVVLYMDPMCLDAFPKLVCFKKRIEAIPQIDKYLKSSKYIA
WPLQGWQATFGGGDHPPKSDLEVLFQGPLGSMEAIAKYDFKATADDELSF
KRGDILKVLNEECDQNWYKAELNGKDGFIPKNYIEMKPHPWFFGKIPRAK
AEEMLSKQRHDGAFLIRESESAPGDFSLSVKFGNDVQHFKVLRDGAGKYF
LWVVKFNSLNELVDYHRSTSVSRNQQIFLRDIEQVPQQPTYVQALFDFDP
QEDGELGFRRGDFIHVMDNSDPNWWKGACHGQTGMFPRNYVTPVNRNV*

• Vector Type: Bacterial
• Antibiotic: Amp
• DU Number: DU31180
• Unit Source: Protein Phosphorylation and Ubiquitylation Unit
• Location: PPU
• MTA: Dundee
• Notes: Bacterial construct to express GST N terminal fusion ; ; Estimated MW of expressed protein : 52029 Daltons