cDNA Clone GRB2

• Plasmid: pEBG6P-1 GRB2
• Parent Plasmid: pEBG6P
• Expressed: GST GRB2
• Genbank: NM_002086.4 GI:156071491
• Tag: GST
• Species: Human

Sequence of Insert

GGATCCATGGAAGCCATCGCCAAATATGACTTCAAAGCTACTGCAGACGA
CGAGCTGAGCTTCAAAAGGGGGGACATCCTCAAGGTTTTGAACGAAGAAT
GTGATCAGAACTGGTACAAGGCAGAGCTTAATGGAAAAGACGGCTTCATT
CCCAAGAACTACATAGAAATGAAACCACATCCGTGGTTTTTTGGCAAAAT
CCCCAGAGCCAAGGCAGAAGAAATGCTTAGCAAACAGCGGCACGATGGGG
CCTTTCTTATCCGAGAGAGTGAGAGCGCTCCTGGGGACTTCTCCCTCTCT
GTCAAGTTTGGAAACGATGTGCAGCACTTCAAGGTGCTCCGAGATGGAGC
CGGGAAGTACTTCCTCTGGGTGGTGAAGTTCAATTCTTTGAATGAGCTGG
TGGATTATCACAGATCTACATCTGTCTCCAGAAACCAGCAGATATTCCTG
CGGGACATAGAACAGGTGCCACAGCAGCCGACATACGTCCAGGCCCTCTT
TGACTTTGATCCCCAGGAGGATGGAGAGCTGGGCTTCCGCCGGGGAGATT
TTATCCATGTCATGGATAACTCAGACCCCAACTGGTGGAAAGGAGCTTGC
CACGGGCAGACCGGCATGTTTCCCCGCAATTATGTCACCCCCGTGAACCG
GAACGTCTAAGCGGCCGC

Amino Acid Sequence

MAPILGYWKIKGLVQPTRLLLEYLEEKYEEHLYERDEGDKWRNKKFELGL
EFPNLPYYIDGDVKLTQSMAIIRYIADKHNMLGGCPKERAEISMLEGAVL
DIRYGVSRIAYSKDFETLKVDFLSKLPEMLKMFEDRLCHKTYLNGDHVTH
PDFMLYDALDVVLYMDPMCLDAFPKLVCFKKRIEAIPQIDKYLKSSKYIA
WPLQGWQATFGGGDHPPKSDLVPRGSLEVLFQGPLGSMEAIAKYDFKATA
DDELSFKRGDILKVLNEECDQNWYKAELNGKDGFIPKNYIEMKPHPWFFG
KIPRAKAEEMLSKQRHDGAFLIRESESAPGDFSLSVKFGNDVQHFKVLRD
GAGKYFLWVVKFNSLNELVDYHRSTSVSRNQQIFLRDIEQVPQQPTYVQA
LFDFDPQEDGELGFRRGDFIHVMDNSDPNWWKGACHGQTGMFPRNYVTPV
NRNV*

• Vector Type: Mammalian
• Antibiotic: Amp
• DU Number: DU31179
• Unit Source: Protein Phosphorylation and Ubiquitylation Unit
• Location: PPU
• MTA: Dundee
• Notes: Mammalian expression plasmid to express GRB2 with N-terminal GST (contains preScission protease site); ; Estimated MW of expressed protein : 52622 Daltons