cDNA Clone DAPK1

• Plasmid: pGEX6P-1-DAPK1
• Parent Plasmid: pGEX6P1
• Expressed: GST-DAPK1
• Genbank: NM_004938.2 GI:89363046
• Tag: GST
• Species: Human

Sequence of Insert

GAATTCATGACCGTGTTCAGGCAGGAAAACGTGGATGATTACTACGACAC
CGGCGAGGAACTTGGCAGTGGACAGTTTGCGGTTGTGAAGAAATGCCGTG
AGAAAAGCACCGGCCTCCAGTATGCCGCCAAATTCATCAAGAAAAGGAGG
ACTAAGTCCAGCCGGCGGGGTGTGAGCCGCGAGGACATCGAGCGGGAGGT
CAGCATCCTGAAGGAGATCCAGCACCCCAATGTCATCACCCTGCACGAGG
TCTATGAGAACAAGACGGACGTCATCCTGATCTTGGAACTCGTTGCAGGT
GGCGAGCTGTTTGACTTCTTAGCTGAAAAGGAATCTTTAACTGAAGAGGA
AGCAACTGAATTTCTCAAACAAATTCTTAATGGTGTTTACTACCTGCACT
CCCTTCAAATCGCCCACTTTGATCTTAAGCCTGAGAACATAATGCTTTTG
GATAGAAATGTCCCCAAACCTCGGATCAAGATCATTGACTTTGGGTTGGC
CCATAAAATTGACTTTGGAAATGAATTTAAAAACATATTTGGGACTCCAG
AGTTTGTCGCTCCTGAGATAGTCAACTATGAACCTCTTGGTCTTGAGGCA
GATATGTGGAGTATCGGGGTAATAACCTATATCCTCCTAAGTGGGGCCTC
CCCATTTCTTGGAGACACTAAGCAAGAAACGTTAGCAAATGTATCCGCTG
TCAACTACGAATTTGAGGATGAATACTTCAGTAATACCAGTGCCCTAGCC
AAAGATTTCATAAGAAGACTTCTGGTCAAGGATCCAAAGAAGAGAATGAC
AATTCAAGATAGTTTGCAGCATCCCTGGATCAAGCCTAAAGATACACAAC
AGGCACTTAGTAGAAAAGCATCAGCAGTAAACATGGAGAAATTCAAGAAG
TTTGCAGCCCGGAAAAAATGGAAACAATCCGTTCGCTTGATATCACTGTG
CCAAAGATTATCCAGGTCATTCCTGTCCAGAAGTAACATGAGTGTTGCCA
GAAGCGATGATACTCTGGATGAGGAAGACTCCTTTGTGATGAAAGCCATC
ATCCATGCCATCAACGATGACAATGTCCCAGGCCTGCAGCACCTTCTGGG
CTCATTATCCAACTATGATGTTAACCAACCCAACAAGCACGGGACACCTC
CATTACTCATTGCTGCTGGCTGTGGGAATATTCAAATACTACAGTTGCTC
ATTAAAAGAGGCTCGAGAATCGATGTCCAGGATAAGGGCGGGTCCAATGC
CGTCTACTGGGCTGCTCGGCATGGCCACGTCGATACCTTGAAATTTCTCA
GTGAGAACAAATGCCCTTTGGATGTGAAAGACAAGTCTGGAGAGATGGCC
CTCCACGTGGCAGCTCGCTATGGCCATGCTGACGTGGCTCAGTTACTGTG
CAGCTTCGGCTCAAATCCCAATATCCAGGACAAGGAAGAAGAAACCCCCC
TGCACTGTGCTGCTTGGCACGGCTATTACTCTGTGGCCAAAGCCCTTTGT
GAAGCCGGCTGTAACGTGAACATCAAGAACCGAGAAGGAGAGACGCCCCT
CCTGACAGCCTCTGCCAGGGGCTACCACGACATCGTGGAGTGTCTGGCCG
AACATGGAGCCGACCTTAATGCTTGCGACAAGGACGGACACATTGCCCTT
CATCTGGCTGTAAGACGGTGTCAGATGGAGGTAATCAAGACTCTCCTCAG
CCAAGGGTGTTTCGTCGATTATCAAGACAGGCACGGCAATACTCCCCTCC
ATGTGGCATGTAAAGATGGCAACATGCCTATCGTGGTGGCCCTCTGTGAA
GCAAACTGCAATTTGGACATCTCCAACAAGTATGGGCGAACGCCTCTGCA
CCTTGCGGCCAACAACGGAATCCTAGACGTGGTCCGGTATCTCTGTCTGA
TGGGAGCCAGCGTTGAGGCGCTGACCACGGACGGAAAGACGGCAGAAGAT
CTTGCTAGATCGGAACAGCACGAGCACGTAGCAGGTCTCCTTGCAAGACT
TCGAAAGGATACGCACCGAGGACTCTTCATCCAGCAGCTCCGACCCACAC
AGAACCTGCAGCCAAGAATTAAGCTCAAGCTGTTTGGCCACTCGGGATCC
GGGAAAACCACCCTTGTAGAATCTCTCAAGTGTGGGCTGCTGAGGAGCTT
TTTCAGAAGGCGTCGGCCCAGACTGTCTTCCACCAACTCCAGCAGGTTCC
CACCTTCACCCCTGGCTTCTAAGCCCACAGTCTCAGTGAGCATCAACAAC
CTGTACCCAGGCTGCGAGAACGTGAGTGTGAGGAGCCGCAGCATGATGTT
CGAGCCGGGTCTTACCAAAGGGATGCTGGAGGTGTTTGTGGCCCCGACCC
ACCACCCGCACTGCTCGGCCGATGACCAGTCCACCAAGGCCATCGACATC
CAGAACGCTTATTTGAATGGAGTTGGCGATTTCAGCGTGTGGGAGTTCTC
TGGAAATCCTGTGTATTTCTGCTGTTATGACTATTTTGCTGCAAATGATC
CCACGTCAATCCATGTTGTTGTCTTTAGTCTAGAAGAGCCCTATGAGATC
CAGCTGAACCAAGTGATTTTCTGGCTCAGTTTCCTGAAGTCCCTTGTCCC
AGTTGAAGAACCCATAGCCTTCGGTGGCAAGCTGAAGAACCCACTCCAAG
TTGTCCTGGTGGCCACCCACGCTGACATCATGAATGTTCCTCGACCGGCT
GGAGGCGAGTTTGGATATGACAAAGACACATCGTTGCTGAAAGAGATTAG
GAACAGGTTTGGAAATGATCTTCACATTTCAAATAAGCTGTTTGTTCTGG
ATGCTGGGGCTTCTGGGTCAAAGGACATGAAGGTACTTCGAAATCATCTG
CAAGAAATACGAAGCCAGATTGTTTCGGTCTGTCCTCCCATGACTCACCT
GTGTGAGAAAATCATCTCCACGCTGCCTTCCTGGAGGAAGCTCAATGGAC
CCAACCAGCTGATGTCGCTGCAGCAGTTTGTGTACGACGTGCAGGACCAG
CTGAACCCCCTGGCCAGCGAGGAGGACCTCAGGCGCATTGCTCAGCAGCT
CCACAGCACAGGCGAGATCAACATCATGCAAAGTGAAACAGTTCAGGACG
TGCTGCTCCTGGACCCCCGCTGGCTCTGCACAAACGTCCTGGGGAAGTTG
CTGTCCGTGGAGACCCCACGGGCGCTGCACCACTACCGGGGCCGCTACAC
CGTGGAGGACATCCAGCGCCTGGTGCCCGACAGCGACGTGGAGGAGCTGC
TGCAGATCCTCGATGCCATGGACATCTGCGCCCGGGACCTGAGCAGCGGG
ACCATGGTGGACGTCCCAGCCCTGATCAAGACAGACAACCTGCACCGCTC
CTGGGCTGATGAGGAGGACGAGGTGATGGTGTATGGTGGCGTGCGCATCG
TGCCCGTGGAACACCTCACCCCCTTCCCATGTGGCATCTTTCACAAGGTC
CAGGTGAACCTGTGCCGGTGGATCCACCAGCAAAGCACAGAGGGCGACGC
GGACATCCGCCTGTGGGTGAATGGCTGCAAGCTGGCCAACCGTGGGGCCG
AGCTGCTGGTGCTGCTGGTCAACCACGGCCAGGGCATTGAGGTCCAGGTC
CGCGGCCTGGAGACGGAGAAGATCAAGTGCTGCCTGCTGCTGGACTCGGT
GTGCAGCACCATTGAGAACGTCATGGCCACCACGCTGCCAGGGCTCCTGA
CCGTGAAGCATTACCTGAGCCCCCAGCAGCTGCGGGAGCACCATGAGCCC
GTCATGATCTACCAGCCACGGGACTTCTTCCGGGCACAGACTCTGAAGGA
AACCTCACTGACCAACACCATGGGGGGGTACAAGGAAAGCTTCAGCAGCA
TCATGTGCTTCGGGTGTCACGACGTCTACTCACAGGCCAGCCTCGGCATG
GACATCCATGCATCAGACCTGAACCTCCTCACTCGGAGGAAACTGAGTCG
CCTGCTGGACCCGCCCGACCCCCTGGGGAAGGACTGGTGCCTTCTCGCCA
TGAACTTAGGCCTCCCTGACCTCGTGGCAAAGTACAACACCAGTAACGGG
GCTCCCAAGGATTTCCTCCCCAGCCCCCTCCACGCCCTGCTGCGGGAATG
GACCACCTACCCTGAGAGCACAGTGGGCACCCTCATGTCCAAACTGAGGG
AGCTGGGTCGCCGGGATGCCGCAGACTTTTTGCTGAAGGCATCCTCTGTG
TTCAAAATCAACCTGGATGGCAATGGCCAGGAGGCCTATGCCTCGAGCTG
CAACAGCGGCACCTCTTACAATTCCATTAGCTCTGTTGTATCCCGGTGAG
CGGCCGC

Amino Acid Sequence

MSPILGYWKIKGLVQPTRLLLEYLEEKYEEHLYERDEGDKWRNKKFELGL
EFPNLPYYIDGDVKLTQSMAIIRYIADKHNMLGGCPKERAEISMLEGAVL
DIRYGVSRIAYSKDFETLKVDFLSKLPEMLKMFEDRLCHKTYLNGDHVTH
PDFMLYDALDVVLYMDPMCLDAFPKLVCFKKRIEAIPQIDKYLKSSKYIA
WPLQGWQATFGGGDHPPKSDLEVLFQGPLGSPEFMTVFRQENVDDYYDTG
EELGSGQFAVVKKCREKSTGLQYAAKFIKKRRTKSSRRGVSREDIEREVS
ILKEIQHPNVITLHEVYENKTDVILILELVAGGELFDFLAEKESLTEEEA
TEFLKQILNGVYYLHSLQIAHFDLKPENIMLLDRNVPKPRIKIIDFGLAH
KIDFGNEFKNIFGTPEFVAPEIVNYEPLGLEADMWSIGVITYILLSGASP
FLGDTKQETLANVSAVNYEFEDEYFSNTSALAKDFIRRLLVKDPKKRMTI
QDSLQHPWIKPKDTQQALSRKASAVNMEKFKKFAARKKWKQSVRLISLCQ
RLSRSFLSRSNMSVARSDDTLDEEDSFVMKAIIHAINDDNVPGLQHLLGS
LSNYDVNQPNKHGTPPLLIAAGCGNIQILQLLIKRGSRIDVQDKGGSNAV
YWAARHGHVDTLKFLSENKCPLDVKDKSGEMALHVAARYGHADVAQLLCS
FGSNPNIQDKEEETPLHCAAWHGYYSVAKALCEAGCNVNIKNREGETPLL
TASARGYHDIVECLAEHGADLNACDKDGHIALHLAVRRCQMEVIKTLLSQ
GCFVDYQDRHGNTPLHVACKDGNMPIVVALCEANCNLDISNKYGRTPLHL
AANNGILDVVRYLCLMGASVEALTTDGKTAEDLARSEQHEHVAGLLARLR
KDTHRGLFIQQLRPTQNLQPRIKLKLFGHSGSGKTTLVESLKCGLLRSFF
RRRRPRLSSTNSSRFPPSPLASKPTVSVSINNLYPGCENVSVRSRSMMFE
PGLTKGMLEVFVAPTHHPHCSADDQSTKAIDIQNAYLNGVGDFSVWEFSG
NPVYFCCYDYFAANDPTSIHVVVFSLEEPYEIQLNQVIFWLSFLKSLVPV
EEPIAFGGKLKNPLQVVLVATHADIMNVPRPAGGEFGYDKDTSLLKEIRN
RFGNDLHISNKLFVLDAGASGSKDMKVLRNHLQEIRSQIVSVCPPMTHLC
EKIISTLPSWRKLNGPNQLMSLQQFVYDVQDQLNPLASEEDLRRIAQQLH
STGEINIMQSETVQDVLLLDPRWLCTNVLGKLLSVETPRALHHYRGRYTV
EDIQRLVPDSDVEELLQILDAMDICARDLSSGTMVDVPALIKTDNLHRSW
ADEEDEVMVYGGVRIVPVEHLTPFPCGIFHKVQVNLCRWIHQQSTEGDAD
IRLWVNGCKLANRGAELLVLLVNHGQGIEVQVRGLETEKIKCCLLLDSVC
STIENVMATTLPGLLTVKHYLSPQQLREHHEPVMIYQPRDFFRAQTLKET
SLTNTMGGYKESFSSIMCFGCHDVYSQASLGMDIHASDLNLLTRRKLSRL
LDPPDPLGKDWCLLAMNLGLPDLVAKYNTSNGAPKDFLPSPLHALLREWT
TYPESTVGTLMSKLRELGRRDAADFLLKASSVFKINLDGNGQEAYASSCN
SGTSYNSISSVVSR*

• Vector Type: Bacterial
• Antibiotic: Amp
• DU Number: DU31172
• Unit Source: Protein Phosphorylation and Ubiquitylation Unit
• Location: PPU
• MTA: Dundee
• Notes: Bacterial construct to express GST N terminal fusion ; ; Estimated MW of expressed protein : 187238 Daltons