cDNA Clone DAPK2

• Plasmid: pGEX6P-DAPK2
• Parent Plasmid: pGEX6P1
• Expressed: GST-DAPK2
• Genbank: NM_014326.3 GI:71774012
• Tag: GST
• Species: Human

Sequence of Insert

GGATCCATGTTCCAGGCCTCAATGAGGAGTCCAAACATGGAGCCATTCAA
GCAGCAGAAGGTGGAGGACTTTTATGACATCGGAGAGGAGCTGGGGAGTG
GCCAGTTTGCCATCGTGAAGAAGTGCCGGGAGAAGAGCACGGGGCTTGAG
TATGCAGCCAAGTTCATCAAGAAGCGGCAGAGCCGGGCGAGCCGGCGCGG
TGTGAGCCGGGAGGAGATCGAGCGGGAGGTGAGCATCCTGCGGCAGGTGC
TGCACCACAATGTCATCACGCTGCACGACGTCTATGAGAACCGCACCGAC
GTGGTGCTCATCCTTGAGCTAGTGTCTGGAGGAGAGCTCTTCGATTTCCT
GGCCCAGAAGGAGTCACTGAGTGAGGAGGAGGCCACCAGCTTCATTAAGC
AGATCCTGGATGGGGTGAACTACCTTCACACAAAGAAAATTGCTCACTTT
GATCTCAAGCCAGAAAACATTATGTTGTTAGACAAGAATATTCCCATTCC
ACACATCAAGCTGATTGACTTTGGTCTGGCTCACGAAATAGAAGATGGAG
TTGAATTTAAGAATATTTTTGGGACGCCGGAATTTGTTGCTCCAGAAATT
GTGAACTACGAGCCCCTGGGTCTGGAGGCTGACATGTGGAGCATAGGCGT
CATCACCTACATCCTCTTAAGTGGAGCATCCCCTTTCCTGGGAGACACGA
AGCAGGAAACACTGGCAAATATCACAGCAGTGAGTTACGACTTTGATGAG
GAATTCTTCAGCCAGACGAGCGAGCTGGCCAAGGACTTTATTCGGAAGCT
TCTGGTTAAAGAGACCCGGAAACGGCTCACAATCCAAGAGGCTCTCAGAC
ACCCCTGGATCACGCCGGTGGACAACCAGCAAGCCATGGTGCGCAGGGAG
TCTGTGGTCAATCTGGAGAACTTCAGGAAGCAGTATGTCCGCAGGCGGTG
GAAGCTTTCCTTCAGCATCGTGTCCCTGTGCAACCACCTCACCCGCTCGC
TGATGAAGAAGGTGCACCTGAGGCCGGATGAGGACCTGAGGAACTGTGAG
AGTGACACTGAGGAGGACATCGCCAGGAGGAAAGCCCTCCACCCACGGAG
GAGGAGCAGCACCTCCTAAGCGGCCGC

Amino Acid Sequence

MSPILGYWKIKGLVQPTRLLLEYLEEKYEEHLYERDEGDKWRNKKFELGL
EFPNLPYYIDGDVKLTQSMAIIRYIADKHNMLGGCPKERAEISMLEGAVL
DIRYGVSRIAYSKDFETLKVDFLSKLPEMLKMFEDRLCHKTYLNGDHVTH
PDFMLYDALDVVLYMDPMCLDAFPKLVCFKKRIEAIPQIDKYLKSSKYIA
WPLQGWQATFGGGDHPPKSDLEVLFQGPLGSMFQASMRSPNMEPFKQQKV
EDFYDIGEELGSGQFAIVKKCREKSTGLEYAAKFIKKRQSRASRRGVSRE
EIEREVSILRQVLHHNVITLHDVYENRTDVVLILELVSGGELFDFLAQKE
SLSEEEATSFIKQILDGVNYLHTKKIAHFDLKPENIMLLDKNIPIPHIKL
IDFGLAHEIEDGVEFKNIFGTPEFVAPEIVNYEPLGLEADMWSIGVITYI
LLSGASPFLGDTKQETLANITAVSYDFDEEFFSQTSELAKDFIRKLLVKE
TRKRLTIQEALRHPWITPVDNQQAMVRRESVVNLENFRKQYVRRRWKLSF
SIVSLCNHLTRSLMKKVHLRPDEDLRNCESDTEEDIARRKALHPRRRSST
S*

• Vector Type: Bacterial
• Antibiotic: Amp
• DU Number: DU31115
• Unit Source: Protein Phosphorylation and Ubiquitylation Unit
• Location: PPU
• MTA: Dundee
• Notes: Bacterial construct to express GST N terminal fusion ; ; Estimated MW of expressed protein : 69720 Daltons