cDNA Clone DAPK1

• Plasmid: pGEX6P-DAPK1 1-363
• Parent Plasmid: pGEX6P1
• Expressed: GST-DAPK1 1-363
• Genbank: NM_004938.2 GI:89363046
• Tag: GST
• Species: Human

Sequence of Insert

GAATTCATGACCGTGTTCAGGCAGGAAAACGTGGATGATTACTACGACAC
CGGCGAGGAACTTGGCAGTGGACAGTTTGCGGTTGTGAAGAAATGCCGTG
AGAAAAGCACCGGCCTCCAGTATGCCGCCAAATTCATCAAGAAAAGGAGG
ACTAAGTCCAGCCGGCGGGGTGTGAGCCGCGAGGACATCGAGCGGGAGGT
CAGCATCCTGAAGGAGATCCAGCACCCCAATGTCATCACCCTGCACGAGG
TCTATGAGAACAAGACGGACGTCATCCTGATCTTGGAACTCGTTGCAGGT
GGCGAGCTGTTTGACTTCTTAGCTGAAAAGGAATCTTTAACTGAAGAGGA
AGCAACTGAATTTCTCAAACAAATTCTTAATGGTGTTTACTACCTGCACT
CCCTTCAAATCGCCCACTTTGATCTTAAGCCTGAGAACATAATGCTTTTG
GATAGAAATGTCCCCAAACCTCGGATCAAGATCATTGACTTTGGGTTGGC
CCATAAAATTGACTTTGGAAATGAATTTAAAAACATATTTGGGACTCCAG
AGTTTGTCGCTCCTGAGATAGTCAACTATGAACCTCTTGGTCTTGAGGCA
GATATGTGGAGTATCGGGGTAATAACCTATATCCTCCTAAGTGGGGCCTC
CCCATTTCTTGGAGACACTAAGCAAGAAACGTTAGCAAATGTATCCGCTG
TCAACTACGAATTTGAGGATGAATACTTCAGTAATACCAGTGCCCTAGCC
AAAGATTTCATAAGAAGACTTCTGGTCAAGGATCCAAAGAAGAGAATGAC
AATTCAAGATAGTTTGCAGCATCCCTGGATCAAGCCTAAAGATACACAAC
AGGCACTTAGTAGAAAAGCATCAGCAGTAAACATGGAGAAATTCAAGAAG
TTTGCAGCCCGGAAAAAATGGAAACAATCCGTTCGCTTGATATCACTGTG
CCAAAGATTATCCAGGTCATTCCTGTCCAGAAGTAACATGAGTGTTGCCA
GAAGCGATGATACTCTGGATGAGGAAGACTCCTTTGTGATGAAAGCCATC
ATCCATGCCATCAACGATGACAATGTCCCAGGCCTGCAGCACCTTTGAGC
GGCCGC

Amino Acid Sequence

MSPILGYWKIKGLVQPTRLLLEYLEEKYEEHLYERDEGDKWRNKKFELGL
EFPNLPYYIDGDVKLTQSMAIIRYIADKHNMLGGCPKERAEISMLEGAVL
DIRYGVSRIAYSKDFETLKVDFLSKLPEMLKMFEDRLCHKTYLNGDHVTH
PDFMLYDALDVVLYMDPMCLDAFPKLVCFKKRIEAIPQIDKYLKSSKYIA
WPLQGWQATFGGGDHPPKSDLEVLFQGPLGSPEFMTVFRQENVDDYYDTG
EELGSGQFAVVKKCREKSTGLQYAAKFIKKRRTKSSRRGVSREDIEREVS
ILKEIQHPNVITLHEVYENKTDVILILELVAGGELFDFLAEKESLTEEEA
TEFLKQILNGVYYLHSLQIAHFDLKPENIMLLDRNVPKPRIKIIDFGLAH
KIDFGNEFKNIFGTPEFVAPEIVNYEPLGLEADMWSIGVITYILLSGASP
FLGDTKQETLANVSAVNYEFEDEYFSNTSALAKDFIRRLLVKDPKKRMTI
QDSLQHPWIKPKDTQQALSRKASAVNMEKFKKFAARKKWKQSVRLISLCQ
RLSRSFLSRSNMSVARSDDTLDEEDSFVMKAIIHAINDDNVPGLQHL*

• Vector Type: Bacterial
• Antibiotic: Amp
• DU Number: DU31113
• Unit Source: Protein Phosphorylation and Ubiquitylation Unit
• Location: PPU
• MTA: Dundee
• Notes: Bacterial construct to express GST N terminal fusion ; ; Estimated MW of expressed protein : 68826 Daltons