cDNA Clone NFKBIL2

• Plasmid: pcDNA5-FRT/TO-GFP NFKBIL2 A714V
• Parent Plasmid: pcDNA5 FRT/TO GFP
• Expressed: GFP NFKBIL2 A714V
• Genbank: NM_013432.4 GI:187608776*
• Tag: GFP
• Species: Human

Sequence of Insert

GGATCCATGAGCCTGGAGCGCGAGCTTCGCCAGCTGAGCAAGGCGAAAGC
CAAGGCGCAGAGGGCCGGGCAGCGGCGCGAAGAGGCCGCGCTGTGCCACC
AGCTGGGGGAGCTCCTGGCCGGCCATGGCCGCTACGCCGAGGCTCTGGAG
CAGCACTGGCAGGAGCTGCAGCTTCGGGAGCGCGCTGACGACCCTCTGGG
CTGTGCCGTGGCCCACCGCAAGATCGGAGAGCGCCTGGCCGAGATGGAGG
ACTACCCGGCTGCCTTGCAGCACCAGCACCAGTACCTGGAGCTGGCACAT
TCCCTGCGCAACCACACGGAGCTGCAGAGGGCCTGGGCCACCATCGGCCG
CACCCACCTGGACATCTATGACCACTGCCAGTCGAGGGATGCTTTGCTGC
AGGCACAGGCTGCCTTTGAGAAGAGCTTGGCTATTGTGGATGAGGAGCTG
GAGGGGACACTGGCCCAGGGAGAGCTGAATGAGATGAGGACCCGCCTCTA
TCTCAACCTGGGCCTCACCTTTGAGAGCCTGCAGCAGACAGCCCTGTGCA
ACGATTACTTCAGGAAGAGCATCTTCCTTGCGGAGCAGAACCACCTTTAC
GAGGACCTATTCCGCGCCCGCTACAACCTGGGCACCATCCACTGGCGCGC
GGGCCAGCACTCCCAGGCTATGCGCTGCTTGGAGGGTGCCCGGGAGTGTG
CGCACACCATGAGGAAGCGGTTCATGGAGAGCGAGTGCTGCGTGGTTATT
GCACAGGTCCTCCAAGACCTGGGAGACTTTTTGGCTGCCAAGCGAGCCCT
GAAGAAGGCCTACAGGCTGGGCTCCCAGAAGCCTGTGCAGAGGGCAGCCA
TCTGTCAGAACCTCCAGCATGTGCTGGCAGTGGTCCGGCTGCAGCAACAG
CTGGAAGAGGCTGAGGGCAGAGACCCTCAGGGTGCCATGGTCATCTGTGA
GCAGCTAGGGGACCTCTTCTCCAAGGCAGGAGACTTTCCCAGGGCAGCTG
AGGCTTACCAGAAGCAGCTGCGTTTTGCTGAGCTGCTGGACAGACCGGGT
GCTGAGCGGGCCATCATCCACGTGTCCCTGGCCACCACACTGGGAGACAT
GAAGGACCACCATGGGGCCGTGCGCCACTATGAGGAGGAACTGAGGCTGC
GCAGCGGCAACGTGCTGGAGGAGGCCAAGACCTGGCTGAACATTGCACTG
TCCCGCGAGGAGGCCGGCGATGCCTACGAGCTGCTGGCCCCGTGCTTCCA
GAAAGCGCTCAGCTGTGCCCAGCAGGCCCAGCGTCCCCAGCTGCAGAGGC
AGGTCTTGCAGCATCTCCATACCGTGCAGCTGAGGCTGCAGCCCCAGGAG
GCCCCTGAGACCGAAACCAGACTGCGGGAGCTCAGTGTAGCTGAAGATGA
AGATGAGGAGGAGGAGGCGGAGGAGGCGGCAGCCACAGCGGAGAGCGAAG
CCCTGGAGGCCGGCGAGGTGGAGCTCTCAGAGGGCGAGGACGACACCGAT
GGCCTGACCCCGCAGCTGGAGGAGGACGAGGAGCTTCAGGGCCACCTGGG
CCGGCGGAAGGGGAGCAAGTGGAACCGGCGAAACGACATGGGGGAGACCC
TGCTGCACCGAGCCTGCATCGAGGGCCAGCTGCGCCGCGTCCAGGACCTT
GTGAGGCAGGGCCACCCCCTTAACCCTCGGGACTACTGTGGCTGGACACC
TCTGCACGAGGCCTGCAACTACGGGCATCTAGAAATTGTCCGCTTCCTGC
TGGACCACGGGGCCGCAGTGGACGACCCAGGTGGCCAGGGCTGCGAAGGC
ATCACCCCCCTCCACGATGCCCTCAACTGTGGCCACTTCGAGGTGGCTGA
GCTGCTGCTTGAACGGGGGGCGTCCGTCACCCTCCGCACTCGAAAGGGCC
TCAGCCCGCTGGAGACGCTGCAGCAGTGGGTGAAGCTGTACCGCAGGGAC
CTGGACCTGGAGACGCGGCAGAAGGCCAGGGCCATGGAGATGCTGCTCCA
GGCGGCTGCCTCGGGCCAAGATCCCCACAGCTCCCAGGCCTTCCACACCC
CAAGCAGCCTTCTGTTTGACCCCGAGACCTCTCCTCCTTTGAGCCCCTGC
CCAGAACCCCCCTCTAATAGCACTAGACTCCCAGAGGCCTCTCAGGTCCA
TGTCAGGGTCTCCCCAGGGCAGGCGGCACCAGCCATGGCCAGGCCTCGGA
GGAGCAGGCATGGGCCAGCCAGCAGCAGCAGCAGCTCAGAAGGCGAGGAC
AGCGCAGGCCCCGCACGGCCGTCCCAGAAGAGGCCTCGGTGCTCGGCCAC
AGCACAACGGGTGGCAGCCTGGACGCCTGGCCCCGCCAGCAACAGGGAAG
CAGCCACAGCCAGCACCAGCCGGGCAGCCTACCAGGCAGCCATCCGGGGT
GTGGGCAGTGCTCAGAGCCGGCTGGGGCCTGGCCCACCGCGGGGCCACAG
CAAAGCCCTTGCCCCCCAGGCAGCGCTCATCCCGGAGGAGGAGTGCCTGG
CTGGGGACTGGCTGGAGCTGGACATGCCCCTGACCCGCAGCCGCCGGCCC
CGCCCCCGGGGCACTGGAGACAACCGCAGGCCCAGTAGTACCTCTGGGTC
GGACAGTGAGGAGAGCAGGCCCCGTGCCCGAGCCAAGCAGGTCCGCCTGA
CCTGCATGCAGAGTTGCAGTGCGCCAGTTAACGCAGGGCCCAGCAGCCTG
GCTTCAGAACCTCCAGGGAGCCCCAGCACCCCCAGGGTCTCAGAGCCCAG
TGGGGACAGCTCTGCGGCAGGCCAGCCCTTGGGTCCGGCCCCGCCCCCTC
CCATCCGGGTTCGAGTTCAAGTTCAGGATCATCTCTTCCTCATCCCTGTC
CCACACAGCAGTGACACCCACTCTGTGGCCTGGCTGGCCGAGCAGGCGGC
CCAGCGCTACTACCAGACCTGCGGGCTGCTGCCCAGGCTCACCCTACGGA
AAGAGGGGGCCCTGCTGGCCCCACAGGACCTCATCCCTGATGTGCTGCAG
AGCAATGACGAGGTGTTGGCTGAGGTGACTTCGTGGGACCTGCCCCCGTT
GACTGACCGCTACCGCAGGGCCTGCCAGAGCCTGGGGCAAGGGGAGCACC
AACAGGTGCTGCAGGCCGTGGAGCTCCAGGGCTTGGGCCTCTCGTTCAGC
GCCTGCTCCCTGGCCCTGGACCAGGCCCAGCTTACACCCCTGCTGCGGGC
CCTCAAGCTGCACACAGCACTCCGGGAGCTGCGCCTGGCAGGGAACCGGC
TGGGGGACAAGTGTGTGGCTGAGCTGGTGGCTGCCCTGGGCACCATGCCC
AGCCTGGCCCTCCTTGACCTCTCCTCCAATCACCTGGGTCCCGAAGGCCT
GCGCCAGCTTGCCATGGGGCTCCCAGGCCAAGCCACCTTGCAGAGTTTGG
AGGAGCTGGACTTAAGCATGAACCCCCTGGGGGACGGCTGTGGCCAGTCC
CTGGCCTCCCTCCTGCACGCCTGCCCCTTACTCAGCACCCTGCGCCTGCA
GGCGTGTGGCTTCGGCCCCAGCTTCTTTCTGAGCCACCAGACAGCACTGG
GTAGTGCTTTCCAAGATGCTGAGCACCTGAAGACCCTGTCCCTGTCCTAC
AACGCCCTGGGAGCCCCTGCCCTGGCCAGGACCCTGCAGAGCCTGCCCGC
CGGCACCCTCCTGCACTTAGAGCTCAGCTCCGTGGCAGCCGGCAAGGGTG
ATTCGGACCTCATGGAGCCTGTATTCCGATACCTGGCCAAGGAAGGCTGT
GCTCTAGCCCACCTGACCCTGTCTGCAAACCACCTGGGGGACAAGGCTGT
TAGAGACCTGTGCAGATGTCTCTCTCTGTGCCCCTCACTCATCTCACTGG
ATCTGTCTGCCAACCCTGAGATCAGCTGTGCCAGCTTGGAAGAGCTCCTG
TCCACCCTCCAAAAGCGGCCCCAAGGCCTTAGCTTCCTTGGCCTGTCAGG
CTGCGCCGTCCAGGGTCCCCTGGGCCTGGGCCTGTGGGACAAGATAGCCG
CGCAGCTCCGGGAACTGCAGCTGTGCAGCAGACGCCTCTGCGCTGAGGAC
AGGGACGCCCTGCGCCAGCTGCAGCCCAGTCGGCCGGGCCCCGGCGAGTG
CACGCTGGACCACGGCTCCAAGCTCTTCTTTCGGCGCCTCTGAGCGGCCG
C

Amino Acid Sequence

MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTLKFICT
TGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIF
FKDDGNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHN
VYIMADKQKNGIKVNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNH
YLSTQSALSKDPNEKRDHMVLLEFVTAAGITLGMDELYKSGLGSMSLERE
LRQLSKAKAKAQRAGQRREEAALCHQLGELLAGHGRYAEALEQHWQELQL
RERADDPLGCAVAHRKIGERLAEMEDYPAALQHQHQYLELAHSLRNHTEL
QRAWATIGRTHLDIYDHCQSRDALLQAQAAFEKSLAIVDEELEGTLAQGE
LNEMRTRLYLNLGLTFESLQQTALCNDYFRKSIFLAEQNHLYEDLFRARY
NLGTIHWRAGQHSQAMRCLEGARECAHTMRKRFMESECCVVIAQVLQDLG
DFLAAKRALKKAYRLGSQKPVQRAAICQNLQHVLAVVRLQQQLEEAEGRD
PQGAMVICEQLGDLFSKAGDFPRAAEAYQKQLRFAELLDRPGAERAIIHV
SLATTLGDMKDHHGAVRHYEEELRLRSGNVLEEAKTWLNIALSREEAGDA
YELLAPCFQKALSCAQQAQRPQLQRQVLQHLHTVQLRLQPQEAPETETRL
RELSVAEDEDEEEEAEEAAATAESEALEAGEVELSEGEDDTDGLTPQLEE
DEELQGHLGRRKGSKWNRRNDMGETLLHRACIEGQLRRVQDLVRQGHPLN
PRDYCGWTPLHEACNYGHLEIVRFLLDHGAAVDDPGGQGCEGITPLHDAL
NCGHFEVAELLLERGASVTLRTRKGLSPLETLQQWVKLYRRDLDLETRQK
ARAMEMLLQAAASGQDPHSSQAFHTPSSLLFDPETSPPLSPCPEPPSNST
RLPEASQVHVRVSPGQAAPAMARPRRSRHGPASSSSSSEGEDSAGPARPS
QKRPRCSATAQRVAAWTPGPASNREAATASTSRAAYQAAIRGVGSAQSRL
GPGPPRGHSKALAPQAALIPEEECLAGDWLELDMPLTRSRRPRPRGTGDN
RRPSSTSGSDSEESRPRARAKQVRLTCMQSCSAPVNAGPSSLASEPPGSP
STPRVSEPSGDSSAAGQPLGPAPPPPIRVRVQVQDHLFLIPVPHSSDTHS
VAWLAEQAAQRYYQTCGLLPRLTLRKEGALLAPQDLIPDVLQSNDEVLAE
VTSWDLPPLTDRYRRACQSLGQGEHQQVLQAVELQGLGLSFSACSLALDQ
AQLTPLLRALKLHTALRELRLAGNRLGDKCVAELVAALGTMPSLALLDLS
SNHLGPEGLRQLAMGLPGQATLQSLEELDLSMNPLGDGCGQSLASLLHAC
PLLSTLRLQACGFGPSFFLSHQTALGSAFQDAEHLKTLSLSYNALGAPAL
ARTLQSLPAGTLLHLELSSVAAGKGDSDLMEPVFRYLAKEGCALAHLTLS
ANHLGDKAVRDLCRCLSLCPSLISLDLSANPEISCASLEELLSTLQKRPQ
GLSFLGLSGCAVQGPLGLGLWDKIAAQLRELQLCSRRLCAEDRDALRQLQ
PSRPGPGECTLDHGSKLFFRRL*

• Vector Type: Mammalian
• Antibiotic: Amp
• DU Number: DU31075
• Unit Source: Protein Phosphorylation and Ubiquitylation Unit
• Location: PPU
• MTA: Dundee
• Notes: *Sequence differences compared with NM_013432.4 CDS:; a1368g (silent), c2141t (A714V), c2496t (silent).