cDNA Clone CREB

• Plasmid: pcDNA5-FRT/TO-HA2 CREB / 4Cre-Luc
• Parent Plasmid: pcDNA5 FRT/TO HA2
• Genbank: CREB: NM_134442.2 GI:22219460
• Tag: HA
• Species: Human

Sequence of Insert

DNA sequence of CREB insert :
GCGGCTGCAATGACCATGGAATCTGGAGCCGAGAACCAGCAGAGTGGAGA
TGCAGCTGTAACAGAAGCTGAAAACCAACAAATGACAGTTCAAGCCCAGC
CACAGATTGCCACATTAGCCCAGGTATCTATGCCAGCAGCTCATGCAACA
TCATCTGCTCCCACCGTAACTCTAGTACAGCTGCCCAATGGGCAGACAGT
TCAAGTCCATGGAGTCATTCAGGCGGCCCAGCCATCAGTTATTCAGTCTC
CACAAGTCCAAACAGTTCAGTCTTCCTGTAAGGACTTAAAAAGACTTTTC
TCCGGAACACAGATTTCAACTATTGCAGAAAGTGAAGATTCACAGGAGTC
AGTGGATAGTGTAACTGATTCCCAAAAGCGAAGGGAAATTCTTTCAAGGA
GGCCTTCCTACAGGAAAATTTTGAATGACTTATCTTCTGATGCACCAGGA
GTGCCAAGGATTGAAGAAGAGAAGTCTGAAGAGGAGACTTCAGCACCTGC
CATCACCACTGTAACGGTGCCAACTCCAATTTACCAAACTAGCAGTGGAC
AGTATATTGCCATTACCCAGGGAGGAGCAATACAGCTGGCTAACAATGGT
ACCGATGGGGTACAGGGCCTGCAAACATTAACCATGACCAATGCAGCAGC
CACTCAGCCGGGTACTACCATTCTACAGTATGCACAGACCACTGATGGAC
AGCAGATCTTAGTGCCCAGCAACCAAGTTGTTGTTCAAGCTGCCTCTGGA
GACGTACAAACATACCAGATTCGCACAGCACCCACTAGCACTATTGCCCC
TGGAGTTGTTATGGCATCCTCCCCAGCACTTCCTACACAGCCTGCTGAAG
AAGCAGCACGAAAGAGAGAGGTCCGTCTAATGAAGAACAGGGAAGCAGCT
CGAGAGTGTCGTAGAAAGAAGAAAGAATATGTGAAATGTTTAGAAAACAG
AGTGGCAGTGCTTGAAAATCAAAACAAGACATTGATTGAGGAGCTAAAAG
CACTTAAGGACCTTTACTGCCACAAATCAGATTAAGCGGCCGC

DNA sequence of 4Cre-luc insert :
gcggccgctcatcaatgtatcttatcatgtctggatccaagctagcctga
cgtcagagagcctgacgtcagagagcctgacgtcagagagcctgacgtca
gagagcggagactctagagggtatataatggaagctcgaattccagcttg
gcattccggtactgttggtaaaatggaagacgccaaaaacataaagaaag
gcccggcgccattctatcctctagaggatggaaccgctggagagcaactg
cataaggctatgaagagatacgccctggttcctggaacaattgcttttac
agatgcacatatcgaggtgaacatcacgtacgcggaatacttcgaaatgt
ccgttcggttggcagaagctatgaaacgatatgggctgaatacaaatcac
agaatcgtcgtatgcagtgaaaactctcttcaattctttatgccggtgtt
gggcgcgttatttatcggagttgcagttgcgcccgcgaacgacatttata
atgaacgtgaattgctcaacagtatgaacatttcgcagcctaccgtagtg
tttgtttccaaaaaggggttgcaaaaaattttgaacgtgcaaaaaaaatt
accaataatccagaaaattattatcatggattctaaaacggattaccagg
gatttcagtcgatgtacacgttcgtcacatctcatctacctcccggtttt
aatgaatacgattttgtaccagagtcctttgatcgtgacaaaacaattgc
actgataatgaattcctctggatctactgggttacctaagggtgtggccc
ttccgcatagaactgcctgcgtcagattctcgcatgccagagatcctatt
tttggcaatcaaatcattccggatactgcgattttaagtgttgttccatt
ccatcacggttttggaatgtttactacactcggatatttgatatgtggat
ttcgagtcgtcttaatgtatagatttgaagaagagctgtttttacgatcc
cttcaggattacaaaattcaaagtgcgttgctagtaccaaccctattttc
attcttcgccaaaagcactctgattgacaaatacgatttatctaatttac
acgaaattgcttctgggggcgcacctctttcgaaagaagtcggggaagcg
gttgcaaaacgcttccatcttccagggatacgacaaggatatgggctcac
tgagactacatcagctattctgattacacccgagggggatgataaaccgg
gcgcggtcggtaaagttgttccattttttgaagcgaaggttgtggatctg
gataccgggaaaacgctgggcgttaatcagagaggcgaattatgtgtcag
aggacctatgattatgtccggttatgtaaacaatccggaagcgaccaacg
ccttgattgacaaggatggatggctacattctggagacatagcttactgg
gacgaagacgaacacttcttcatagttgaccgcttgaagtctttaattaa
atacaaaggatatcaggtggcccccgctgaattggaatcgatattgttac
aacaccccaacatcttcgacgcgggcgtggcaggtcttcccgacgatgac
gccggtgaacttcccgccgccgttgttgttttggagcacggaaagacgat
gacggaaaaagagatcgtggattacgtcgccagtcaagtaacaaccgcga
aaaagttgcgcggaggagttgtgtttgtggacgaagtaccgaaaggtctt
accggaaaactcgacgcaagaaaaatcagagagatcctcataaaggccaa
gaagggcggaaagtccaaattgtaagcggccgc

Amino Acid Sequence

Amino acid sequence of expressed HA-CREB protein :
MYPYDVPDYAGIPGIPGSTRAAAMTMESGAENQQSGDAAVTEAENQQMTV
QAQPQIATLAQVSMPAAHATSSAPTVTLVQLPNGQTVQVHGVIQAAQPSV
IQSPQVQTVQSSCKDLKRLFSGTQISTIAESEDSQESVDSVTDSQKRREI
LSRRPSYRKILNDLSSDAPGVPRIEEEKSEEETSAPAITTVTVPTPIYQT
SSGQYIAITQGGAIQLANNGTDGVQGLQTLTMTNAAATQPGTTILQYAQT
TDGQQILVPSNQVVVQAASGDVQTYQIRTAPTSTIAPGVVMASSPALPTQ
PAEEAARKREVRLMKNREAARECRRKKKEYVKCLENRVAVLENQNKTLIE
ELKALKDLYCHKSD*

Amino acid sequence of Luc protein :
MEARIPAWHSGTVGKMEDAKNIKKGPAPFYPLEDGTAGEQLHKAMKRYAL
VPGTIAFTDAHIEVNITYAEYFEMSVRLAEAMKRYGLNTNHRIVVCSENS
LQFFMPVLGALFIGVAVAPANDIYNERELLNSMNISQPTVVFVSKKGLQK
ILNVQKKLPIIQKIIIMDSKTDYQGFQSMYTFVTSHLPPGFNEYDFVPES
FDRDKTIALIMNSSGSTGLPKGVALPHRTACVRFSHARDPIFGNQIIPDT
AILSVVPFHHGFGMFTTLGYLICGFRVVLMYRFEEELFLRSLQDYKIQSA
LLVPTLFSFFAKSTLIDKYDLSNLHEIASGGAPLSKEVGEAVAKRFHLPG
IRQGYGLTETTSAILITPEGDDKPGAVGKVVPFFEAKVVDLDTGKTLGVN
QRGELCVRGPMIMSGYVNNPEATNALIDKDGWLHSGDIAYWDEDEHFFIV
DRLKSLIKYKGYQVAPAELESILLQHPNIFDAGVAGLPDDDAGELPAAVV
VLEHGKTMTEKEIVDYVASQVTTAKKLRGGVVFVDEVPKGLTGKLDARKI
REILIKAKKGGKSKL*

• Vector Type: Mammalian
• Antibiotic: Amp
• DU Number: DU16857
• Unit Source: Protein Phosphorylation and Ubiquitylation Unit
• Location: PPU
• MTA: Dundee
• Notes: 5’ Not1 site in pcDNA5 FRT/TO HA2 CREB (DU16616) killed by mutagenesis. 4Cre-luc cloned into 3’ Not1 site in reverse orientation with respect to CREB.