cDNA Clone FCHSD2

• Plasmid: pEBG6P-1 FCHSD2
• Parent Plasmid: pEBG6P
• Expressed: GST FCHSD2
• Genbank: NM_014824.2 GI:182765465
• Tag: GST
• Species: Human

Sequence of Insert

GGATCCATGCAGCCGCCGCCGAGGAAGGTGAAAGTTACACAAGAACTGAA
AAACATTCAAGTTGAGCAGATGACAAAACTTCAAGCCAAACATCAAGCAG
AATGTGATTTGCTTGAAGATATGAGGACATTCAGTCAGAAGAAGGCTGCT
ATTGAAAGAGAGTATGCACAGGGTATGCAGAAGTTGGCTAGTCAATACCT
GAAGAGAGATTGGCCTGGAGTAAAAGCTGATGATCGGAATGATTACAGGA
GCATGTATCCCGTTTGGAAATCTTTTCTCGAGGGAACAATGCAGGTAGCC
CAGTCTCGGATGAATATATGTGAAAACTATAAAAACTTCATTTCTGAGCC
TGCAAGGACAGTGAGAAGCTTAAAAGAACAGCAACTAAAAAGGTGTGTGG
ACCAGTTGACAAAGATCCAAACTGAATTACAAGAGACAGTGAAAGATTTA
GCTAAAGGCAAAAAGAAATACTTTGAGACTGAACAGATGGCTCATGCAGT
ACGAGAGAAAGCTGACATCGAGGCAAAATCTAAACTTAGTCTTTTTCAAT
CAAGAATCAGTTTACAGAAGGCAAGTGTAAAGTTAAAAGCCCGGCGATCT
GAGTGTAATTCCAAAGCTACCCACGCAAGGAATGATTATCTTCTTACCCT
AGCGGCAGCAAATGCACATCAGGATCGCTACTATCAAACAGATTTAGTTA
ACATTATGAAGGCTCTTGATGGAAATGTGTATGATCATCTCAAGGATTAT
TTAATAGCCTTCAGCCGGACTGAGCTAGAAACATGCCAAGCTGTGCAGAA
CACATTCCAGTTTTTATTAGAAAACTCCAGCAAGGTGGTCCGGGACTACA
ATCTTCAGCTGTTTTTGCAAGAAAACGCTGTATTTCACAAACCCCAGCCC
TTCCAGTTCCAGCCTTGTGACAGTGATACTAGCCGACAGTTAGAATCAGA
AACTGGGACCACAGAGGAGCACAGTCTAAATAAGGAAGCTCGAAAATGGG
CCACACGTGTGGCACGTGAGCATAAAAACATTGTTCACCAACAACGGGTT
CTAAATGATCTGGAGTGTCATGGAGCTGCTGTATCAGAACAAAGCCGAGC
AGAGCTAGAACAGAAAATAGATGAAGCTAGAGAAAATATTCGTAAAGCAG
AGATAATTAAATTGAAAGCTGAAGCCCGGTTGGACCTGCTAAAGCAGATT
GGTGTTTCTGTGGACACATGGCTAAAGAGTGCCATGAACCAAGTAATGGA
AGAACTGGAAAATGAGCGATGGGCCCGCCCTCCTGCAGTGACCAGTAATG
GCACTTTACACTCGCTTAATGCAGATACCGAAAGAGAAGAAGGCGAAGAG
TTTGAAGATAACATGGATGTTTTCGATGACAGCAGTTCCAGCCCTTCTGG
CACCTTAAGAAATTATCCACTCACCTGCAAAGTTGTTTATTCCTACAAGG
CTTCTCAACCAGATGAGTTGACCATTGAGGAACATGAGGTGTTAGAAGTG
ATTGAAGATGGAGATATGGAAGACTGGGTAAAGGCTCGAAATAAAGTTGG
CCAAGTGGGTTATGTGCCAGAAAAGTACCTACAGTTTCCCACCTCGAACA
GCCTCCTGAGCATGCTGCAGTCCCTGGCCGCTTTGGACAGTCGGTCACAC
ACGTCCAGCAATTCCACGGAAGCAGAACTCGTTTCAGGCAGCCTCAACGG
AGATGCCAGTGTATGTTTTGTGAAAGCACTTTATGATTATGAGGGCCAGA
CAGATGATGAGTTATCTTTTCCTGAGGGAGCAATAATCCGTATCTTGAAC
AAAGAAAACCAAGATGATGATGGCTTCTGGGAAGGGGAATTCAATGGGCG
TATTGGAGTTTTCCCATCGGTGCTAGTGGAAGAACTTTCAGCCTCAGAAA
ATGGTGACACTCCATGGATGAGAGAGATTCAGATCTCTCCTTCCCCCAAG
CCACACGCCTCCCTGCCTCCACTGCCGTTGTACGACCAGCCTCCCAGCAG
CCCCTACCCCAGCCCAGATAAGAGGAGCTCCCTGTACTTTCCCCGGTCTC
CTTCAGCAAACGAAAAAAGCCTTCATGCTGAGTCACCAGGATTCTCACAG
GCATCAAGGCATACTCCTGAGACCTCATATGGCAAACTGCGACCTGTCCG
GGCAGCTCCCCCTCCACCTACACAGAATCACCGAAGGCCAGCAGAGAAGA
TTGAAGATGTGGAAATCACACTGGTGTGAGCGGCCGC

Amino Acid Sequence

MAPILGYWKIKGLVQPTRLLLEYLEEKYEEHLYERDEGDKWRNKKFELGL
EFPNLPYYIDGDVKLTQSMAIIRYIADKHNMLGGCPKERAEISMLEGAVL
DIRYGVSRIAYSKDFETLKVDFLSKLPEMLKMFEDRLCHKTYLNGDHVTH
PDFMLYDALDVVLYMDPMCLDAFPKLVCFKKRIEAIPQIDKYLKSSKYIA
WPLQGWQATFGGGDHPPKSDLVPRGSLEVLFQGPLGSMQPPPRKVKVTQE
LKNIQVEQMTKLQAKHQAECDLLEDMRTFSQKKAAIEREYAQGMQKLASQ
YLKRDWPGVKADDRNDYRSMYPVWKSFLEGTMQVAQSRMNICENYKNFIS
EPARTVRSLKEQQLKRCVDQLTKIQTELQETVKDLAKGKKKYFETEQMAH
AVREKADIEAKSKLSLFQSRISLQKASVKLKARRSECNSKATHARNDYLL
TLAAANAHQDRYYQTDLVNIMKALDGNVYDHLKDYLIAFSRTELETCQAV
QNTFQFLLENSSKVVRDYNLQLFLQENAVFHKPQPFQFQPCDSDTSRQLE
SETGTTEEHSLNKEARKWATRVAREHKNIVHQQRVLNDLECHGAAVSEQS
RAELEQKIDEARENIRKAEIIKLKAEARLDLLKQIGVSVDTWLKSAMNQV
MEELENERWARPPAVTSNGTLHSLNADTEREEGEEFEDNMDVFDDSSSSP
SGTLRNYPLTCKVVYSYKASQPDELTIEEHEVLEVIEDGDMEDWVKARNK
VGQVGYVPEKYLQFPTSNSLLSMLQSLAALDSRSHTSSNSTEAELVSGSL
NGDASVCFVKALYDYEGQTDDELSFPEGAIIRILNKENQDDDGFWEGEFN
GRIGVFPSVLVEELSASENGDTPWMREIQISPSPKPHASLPPLPLYDQPP
SSPYPSPDKRSSLYFPRSPSANEKSLHAESPGFSQASRHTPETSYGKLRP
VRAAPPPPTQNHRRPAEKIEDVEITLV*

• Vector Type: Mammalian
• Antibiotic: Amp
• DU Number: DU16813
• Unit Source: Protein Phosphorylation and Ubiquitylation Unit
• Location: PPU
• MTA: Dundee
• Notes: Mammalian expression plasmid to express FCHSD2 with N-terminal GST (contains preScission protease site); ; Estimated MW of expressed protein : 111690 Daltons