cDNA Clone FCHSD1

• Plasmid: pcDNA5-FRT/TO-GFP FCHSD1
• Parent Plasmid: pcDNA5 FRT/TO GFP
• Expressed: GFP FCHSD1
• Genbank: NM_033449.1 GI:28875785*
• Tag: GFP
• Species: Human

Sequence of Insert

AGATCTATGCAGCCGCCGCCCCGAAAAGTGAAGCCGGCCCAGGAGGTGAA
GCTTCGCTTCCTGGAACAGCTGAGCATCCTTCAGACCTGGCAGCAGAGGG
AGGCGGATCTGCTGGAGGACATCAGATCCTACAGCAAGCAGAGGGCAGCC
ATTGAACGGGAGTATGGGCAGGCACTCCAGAAACTGGCTGGCCCATTCCT
GAAGAGGGAAGGGCACCGGAGCGGTGAGATGGACAGCAGGACAGTGTTCG
GTGCCTGGCGCTGCCTGCTGGATGCCACCGTGGCTGGGGGCCAAACCCGA
CTCCAGGCGTCTGACCGATACCGTGACCTAGCAGGGGGTACAGGGCGGAG
CGCCAAGGAGCAGGTGCTTAGGAAGGGAACAGAGAACCTCCAGAGGGCGC
AGGCTGAGGTGCTGCAGTCTGTCCGGGAGCTGAGCCGAAGTCGGAAGCTG
TATGGGCAGCGGGAACGTGTGTGGGCCTTGGCACAGGAGAAGGCGGCTGA
TGTCCAGGCCAGGCTAAACCGAAGTGACCATGGGATCTTCCACTCTCGGA
CCAGTCTCCAGAAACTGAGCACCAAGCTGTCCGCCCAGTCAGCCCAGTAC
TCCCAGCAGCTGCAAGCAGCCCGCAATGAGTACCTGCTTAACTTGGTGGC
TACCAATGCCCACCTCGACCATTACTACCAGGAGGAACTGCCAGCTCTGC
TCAAGGCCCTGGTCAGTGAGCTGTCAGAGCACTTGAGGGACCCCCTGACC
TCCCTGAGCCACACTGAGCTGGAAGCCGCAGAGGTCATCCTGGAGCATGC
CCACCGCGGGGAGCAGACAACCTCCCAGGTAAGCTGGGAGCAAGACCTGA
AGCTGTTTCTTCAGGAGCCTGGTGTATTTTCCCCCACCCCACCTCAGCAG
TTTCAGCCAGCAGGGACTGATCAGGTGTGTGTCCTGGAGTGGGGAGCAGA
AGGCGTGGCTGGCAAGAGTGGCCTGGAGAAAGAGGTTCAGCGCTTGACCA
GCCGAGCTGCCCGTGACTACAAGATCCAGAACCATGGGCATCGGGTACTG
CAACGACTGGAGCAGAGGCGGCAGCAGGCTTCAGAGCGGGAGGCTCCAAG
CATAGAACAGAGGTTACAGGAAGTGCGAGAGAGCATCCGCCGGGCACAGG
TGAGCCAGGTGAAGGGGGCTGCCCGGCTGGCCCTGCTGCAGGGGGCTGGC
TTAGATGTGGAGCGCTGGCTGAAGCCAGCCATGACCCAGGCCCAGGATGA
GGTGGAGCAGGAGCGGCGGCTCAGTGAGGCTCGGCTGTCCCAGAGGGACC
TCTCTCCAACCGCTGAGGATGCTGAGCTTTCTGACTTTGAGGAATGTGAG
GAGACGGGAGAGCTCTTTGAGGAGCCTGCCCCCCAAGCCCTGGCCACGAG
GGCCCTCCCCTGCCCTGCACACGTGGTATTTCGCTATCAGGCAGGGCGTG
AGGATGAGCTGACAATCACGGAGGGTGAGTGGCTGGAGGTCATAGAGGAG
GGAGATGCTGACGAATGGGTCAAGGCTCGGAACCAGCACGGCGAGGTAGG
CTTTGTCCCTGAGCGATATCTCAACTTCCCGGACCTCTCCCTCCCAGAGA
GCAGCCAAGACAGTGACAATCCCTGCGGGGCAGAGCCCACAGCATTCCTG
GCACAGGCCCTGTACAGCTACACCGGACAGAGTGCAGAGGAGCTGAGCTT
CCCTGAGGGGGCACTCATCCGTCTGCTGCCCCGGGCCCAAGATGGAGTAG
ATGACGGCTTCTGGAGGGGAGAATTTGGGGGCCGTGTTGGGGTCTTCCCC
TCCCTGCTGGTGGAAGAGCTGCTTGGCCCCCCAGGGCCACCTGAACTCTC
TGACCCTGAACAGATGCTGCCGTCCCCTTCTCCTCCCAGCTTCTCCCCAC
CTGCACCTACCTCTGTGTTGGATGGGCCCCCTGCACCTGTCCTGCCTGGG
GACAAAGCCCTGGACTTCCCTGGGTTCCTGGACATGATGGCACCTCGACT
CAGGCCGATGCGTCCACCACCTCCCCCGCCGGCTAAAGCCCCGGATCCTG
GCCACCCAGATCCCCTCACCTGAGCGGCCGC

Amino Acid Sequence

MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTLKFICT
TGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIF
FKDDGNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHN
VYIMADKQKNGIKVNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNH
YLSTQSALSKDPNEKRDHMVLLEFVTAAGITLGMDELYKSGLGSMQPPPR
KVKPAQEVKLRFLEQLSILQTWQQREADLLEDIRSYSKQRAAIEREYGQA
LQKLAGPFLKREGHRSGEMDSRTVFGAWRCLLDATVAGGQTRLQASDRYR
DLAGGTGRSAKEQVLRKGTENLQRAQAEVLQSVRELSRSRKLYGQRERVW
ALAQEKAADVQARLNRSDHGIFHSRTSLQKLSTKLSAQSAQYSQQLQAAR
NEYLLNLVATNAHLDHYYQEELPALLKALVSELSEHLRDPLTSLSHTELE
AAEVILEHAHRGEQTTSQVSWEQDLKLFLQEPGVFSPTPPQQFQPAGTDQ
VCVLEWGAEGVAGKSGLEKEVQRLTSRAARDYKIQNHGHRVLQRLEQRRQ
QASEREAPSIEQRLQEVRESIRRAQVSQVKGAARLALLQGAGLDVERWLK
PAMTQAQDEVEQERRLSEARLSQRDLSPTAEDAELSDFEECEETGELFEE
PAPQALATRALPCPAHVVFRYQAGREDELTITEGEWLEVIEEGDADEWVK
ARNQHGEVGFVPERYLNFPDLSLPESSQDSDNPCGAEPTAFLAQALYSYT
GQSAEELSFPEGALIRLLPRAQDGVDDGFWRGEFGGRVGVFPSLLVEELL
GPPGPPELSDPEQMLPSPSPPSFSPPAPTSVLDGPPAPVLPGDKALDFPG
FLDMMAPRLRPMRPPPPPPAKAPDPGHPDPLT*

• Vector Type: Mammalian
• Antibiotic: Amp
• Full Snapgene Map: pcDNA5-FRT/TO-GFP FCHSD155.19 KB Snapgene files can be opened using software available here: www.snapgene.com
• DU Number: DU16675
• Unit Source: Protein Phosphorylation and Ubiquitylation Unit
• Location: PPU
• MTA: Dundee
• Notes: * Lacks residues G79 and R80 compared with NM_033449.1 sequence
• Low Temp: No