cDNA Clone FCHSD1

• Plasmid: pEBG6P-1 FCHSD1
• Parent Plasmid: pEBG6P
• Expressed: GST-FCHSD1
• Genbank: NM_033449.1 GI:28875785*
• Tag: GST
• Species: Human

Sequence of Insert

AGATCTATGCAGCCGCCGCCCCGAAAAGTGAAGCCGGCCCAGGAGGTGAA
GCTTCGCTTCCTGGAACAGCTGAGCATCCTTCAGACCTGGCAGCAGAGGG
AGGCGGATCTGCTGGAGGACATCAGATCCTACAGCAAGCAGAGGGCAGCC
ATTGAACGGGAGTATGGGCAGGCACTCCAGAAACTGGCTGGCCCATTCCT
GAAGAGGGAAGGGCACCGGAGCGGTGAGATGGACAGCAGGACAGTGTTCG
GTGCCTGGCGCTGCCTGCTGGATGCCACCGTGGCTGGGGGCCAAACCCGA
CTCCAGGCGTCTGACCGATACCGTGACCTAGCAGGGGGTACAGGGCGGAG
CGCCAAGGAGCAGGTGCTTAGGAAGGGAACAGAGAACCTCCAGAGGGCGC
AGGCTGAGGTGCTGCAGTCTGTCCGGGAGCTGAGCCGAAGTCGGAAGCTG
TATGGGCAGCGGGAACGTGTGTGGGCCTTGGCACAGGAGAAGGCGGCTGA
TGTCCAGGCCAGGCTAAACCGAAGTGACCATGGGATCTTCCACTCTCGGA
CCAGTCTCCAGAAACTGAGCACCAAGCTGTCCGCCCAGTCAGCCCAGTAC
TCCCAGCAGCTGCAAGCAGCCCGCAATGAGTACCTGCTTAACTTGGTGGC
TACCAATGCCCACCTCGACCATTACTACCAGGAGGAACTGCCAGCTCTGC
TCAAGGCCCTGGTCAGTGAGCTGTCAGAGCACTTGAGGGACCCCCTGACC
TCCCTGAGCCACACTGAGCTGGAAGCCGCAGAGGTCATCCTGGAGCATGC
CCACCGCGGGGAGCAGACAACCTCCCAGGTAAGCTGGGAGCAAGACCTGA
AGCTGTTTCTTCAGGAGCCTGGTGTATTTTCCCCCACCCCACCTCAGCAG
TTTCAGCCAGCAGGGACTGATCAGGTGTGTGTCCTGGAGTGGGGAGCAGA
AGGCGTGGCTGGCAAGAGTGGCCTGGAGAAAGAGGTTCAGCGCTTGACCA
GCCGAGCTGCCCGTGACTACAAGATCCAGAACCATGGGCATCGGGTACTG
CAACGACTGGAGCAGAGGCGGCAGCAGGCTTCAGAGCGGGAGGCTCCAAG
CATAGAACAGAGGTTACAGGAAGTGCGAGAGAGCATCCGCCGGGCACAGG
TGAGCCAGGTGAAGGGGGCTGCCCGGCTGGCCCTGCTGCAGGGGGCTGGC
TTAGATGTGGAGCGCTGGCTGAAGCCAGCCATGACCCAGGCCCAGGATGA
GGTGGAGCAGGAGCGGCGGCTCAGTGAGGCTCGGCTGTCCCAGAGGGACC
TCTCTCCAACCGCTGAGGATGCTGAGCTTTCTGACTTTGAGGAATGTGAG
GAGACGGGAGAGCTCTTTGAGGAGCCTGCCCCCCAAGCCCTGGCCACGAG
GGCCCTCCCCTGCCCTGCACACGTGGTATTTCGCTATCAGGCAGGGCGTG
AGGATGAGCTGACAATCACGGAGGGTGAGTGGCTGGAGGTCATAGAGGAG
GGAGATGCTGACGAATGGGTCAAGGCTCGGAACCAGCACGGCGAGGTAGG
CTTTGTCCCTGAGCGATATCTCAACTTCCCGGACCTCTCCCTCCCAGAGA
GCAGCCAAGACAGTGACAATCCCTGCGGGGCAGAGCCCACAGCATTCCTG
GCACAGGCCCTGTACAGCTACACCGGACAGAGTGCAGAGGAGCTGAGCTT
CCCTGAGGGGGCACTCATCCGTCTGCTGCCCCGGGCCCAAGATGGAGTAG
ATGACGGCTTCTGGAGGGGAGAATTTGGGGGCCGTGTTGGGGTCTTCCCC
TCCCTGCTGGTGGAAGAGCTGCTTGGCCCCCCAGGGCCACCTGAACTCTC
TGACCCTGAACAGATGCTGCCGTCCCCTTCTCCTCCCAGCTTCTCCCCAC
CTGCACCTACCTCTGTGTTGGATGGGCCCCCTGCACCTGTCCTGCCTGGG
GACAAAGCCCTGGACTTCCCTGGGTTCCTGGACATGATGGCACCTCGACT
CAGGCCGATGCGTCCACCACCTCCCCCGCCGGCTAAAGCCCCGGATCCTG
GCCACCCAGATCCCCTCACCTGAGCGGCCGC

Amino Acid Sequence

MAPILGYWKIKGLVQPTRLLLEYLEEKYEEHLYERDEGDKWRNKKFELGL
EFPNLPYYIDGDVKLTQSMAIIRYIADKHNMLGGCPKERAEISMLEGAVL
DIRYGVSRIAYSKDFETLKVDFLSKLPEMLKMFEDRLCHKTYLNGDHVTH
PDFMLYDALDVVLYMDPMCLDAFPKLVCFKKRIEAIPQIDKYLKSSKYIA
WPLQGWQATFGGGDHPPKSDLVPRGSLEVLFQGPLGSMQPPPRKVKPAQE
VKLRFLEQLSILQTWQQREADLLEDIRSYSKQRAAIEREYGQALQKLAGP
FLKREGHRSGEMDSRTVFGAWRCLLDATVAGGQTRLQASDRYRDLAGGTG
RSAKEQVLRKGTENLQRAQAEVLQSVRELSRSRKLYGQRERVWALAQEKA
ADVQARLNRSDHGIFHSRTSLQKLSTKLSAQSAQYSQQLQAARNEYLLNL
VATNAHLDHYYQEELPALLKALVSELSEHLRDPLTSLSHTELEAAEVILE
HAHRGEQTTSQVSWEQDLKLFLQEPGVFSPTPPQQFQPAGTDQVCVLEWG
AEGVAGKSGLEKEVQRLTSRAARDYKIQNHGHRVLQRLEQRRQQASEREA
PSIEQRLQEVRESIRRAQVSQVKGAARLALLQGAGLDVERWLKPAMTQAQ
DEVEQERRLSEARLSQRDLSPTAEDAELSDFEECEETGELFEEPAPQALA
TRALPCPAHVVFRYQAGREDELTITEGEWLEVIEEGDADEWVKARNQHGE
VGFVPERYLNFPDLSLPESSQDSDNPCGAEPTAFLAQALYSYTGQSAEEL
SFPEGALIRLLPRAQDGVDDGFWRGEFGGRVGVFPSLLVEELLGPPGPPE
LSDPEQMLPSPSPPSFSPPAPTSVLDGPPAPVLPGDKALDFPGFLDMMAP
RLRPMRPPPPPPAKAPDPGHPDPLT*

• Vector Type: Mammalian
• Antibiotic: Amp
• DU Number: DU16674
• Unit Source: Protein Phosphorylation and Ubiquitylation Unit
• Location: PPU
• MTA: Dundee
• Notes: Mammalian expression plasmid to express FCHSD1 with N-terminal GST (contains preScission protease site); Sequence difference compared with NM_033449.1 CDS:; delta gcaggg at nt 230-235 (delta R78 and G79) this deletion present in majority of ESTs.; ; Estimated MW of expressed protein : 104142 Daltons