cDNA Clone GRIPE

• Plasmid: pcDNA5-FRT/TO-GFP GRIPE 1-1172
• Parent Plasmid: pcDNA5 FRT/TO GFP
• Expressed: GFP GRIPE 1-1172
• Genbank: NM_194301.2 GI:51230411
• Tag: GFP
• Species: Human

Sequence of Insert

GCGGCCGCAATGTTCTCCAAGAAGCCGCACGGGGACGTGAAGAAGTCCAC
CCAGAAGGTGCTAGACACCAAGAAGGACGCACTGACTCGCCTCAAGCACC
TGCGCATCGTCATCGAGAATGCAGAATCTATTGATCTTAAACAGTTTTTC
GACCAACATTTTTCACATATATACTATGTGTTCTTTGAAAATTTTGTGAC
TATTGAAGCTAGTCTTAAACAGAAAGGTCACAAGTCTCAAAGAGAGGAAT
TGGATGCTATACTTTTTATTTTTGAGAAAATTTTACAACTTCTTCCAGAA
AGAATTCATCAGCGATGGCAGTTTCATAGTATTGGATTGATTTTAAAGAA
GCTACTTCACACAGGAAACTCCTTAAAGATTAGGCGGGAAGGTGTTCGTC
TTTTCTTACTATGGTTGCAAGCTCTTCAGAATAACTGTAGCAAAGAACAG
CTCTGGATGTTTTCATGCTTAATCCCTGGATTTTCAGCACCACAGTCTGA
ACATGGACCTCGAACTTTAGATAATCTCATTAATCCTCCACTCAACCTTC
AAGAAACTCAAGTCACTATAGAAGAAATCACTCCTCTTGTCCCCCCACAA
TCAGGAGATAAAGGGCAAGAAGATCTCACAAGCTATTTTCTTGAAGCACT
TCTAAAATACATAGTCATTCAGGTAAAAAGTTTAGAATGGAAGAACAAAG
AAAACCAAGAAAGGGGATTTTCATTTTTGTTTTCACATTTTAAGAAATAT
TACTTGCCTTATATTTTTCCAAACATCTGTAAGGAAAACAGTTTATATCA
TCCTATACTTGACATCCCGCAGATGAGACCAAAGCCACATTATGTCGTGA
TAAAGAAAGATGCTGAAACCAATGAAGCAATCTATTGTACAAAGGAGCCT
TTCATTAAGGCTCGTGTTATTGTCATTCGTTGGCTGGTTTCTTTCTGGCT
GGAGCCAAAACCACATACAGGACCTCATATTCCTGGGATGGAAGGTGAAG
TCTTGCCAAAGAATATTCAGAGAGCAGCTGCTAGTTTAGTATCCAGAGAA
GAAAGCAAAAATGATAATGCTGATAAAACAGACAGAACTACAGAACCCGA
ACAGTCTCATTCCAATACAAGCACTCTCACGGAGCGAGAACCTAGCTCAT
CTAGTCTCTGTAGTATTGATGAAGAACATCTCACAGACATTGAAATAGTT
CGCAGAGTTTTTTCTTCTAAAAGGAGTAATGTAAACTTTGTGACAGAGAT
ATTTCGTCAGGCATTTTTATTACCAATTTGTGAAGCAGCAGCTATGAGAA
AAGTGGTAAAAGTATATCAAGAATGGATCCAACAAGAGGAAAAACCTTTG
TTCATGCAAGAGCCTGAAGAAATTGTGATCACTTCTTCAGACCTCCCTTG
CATTGAAAATGTCACAGACCATGATATTTCAATGGAAGAAGGAGAAAAAA
GAGAAGAGGAAAATGGGACCAATACTGCTGATCATGTTCGAAATTCCAGT
TGGGCAAAAAACGGCTCCTACCAAGGTGCTCTTCATAACGCCTCTGAAGA
AGCCACAGAACAAAACATACGAGCTGGTACCCAGGCAGTTTTGCAGGTGT
TTATTATAAACTCATCAAATATATTTCTTCTTGAACCTGCAAATGAAATA
AAAAATCTTCTGGATGAACACACAGATATGTGTAAACGCATTCTTAACAT
TTATCGGTACATGGTTGTACAAGTATCAATGGACAAAAAGACTTGGGAAC
AGATGCTGCTTGTGTTGCTCAGAGTCACGGAATCTGTACTGAAGATGCCA
TCACAAGCTTTTCTACAGTTCCAAGGGAAAAAAAATATGACCTTGGCAGG
TCGACTTGCAGGACCACTTTTCCAGACCCTTATAGTTGCCTGGATCAAAG
CAAACCTAAATGTGTACATCTCCCGAGAACTTTGGGATGACTTACTGTCA
GTATTGTCATCGCTGACCTATTGGGAAGAGTTGGCCACTGAGTGGTCACT
GACTATGGAGACATTAACTAAAGTTTTAGCTAGGAATTTATATAGTTTGG
ATCTCAGTGATTTACCATTGGATAAGCTGAGTGAACAGAAACAAAAAAAG
CACAAAGGGAAAGGAGTTGGACATGAATTTCAGAAAGTTTCAGTTGACAA
GTCATTTTCTAGAGGATGGAGTCGTGATCAGCCTGGCCAAGCCCCAATGA
GACAGAGGAGTGCAACAACCACTGGTTCTCCAGGAACCGAAAAGGCGAGG
AGTATAGTACGGCAAAAAACTGTCGATATTGATGATGCTCAAATACTTCC
CCGCTCAACTAGAGTCAGACATTTTTCACAAAGTGAAGAAACTGGAAATG
AAGTTTTTGGTGCTTTGAATGAGGAGCAGCCATTGCCTCGAAGTAGCAGC
ACTTCTGACATCTTGGAACCATTCACTGTTGAACGAGCCAAAGTCAATAA
AGAGGACATGAGCCAAAAACTGCCTCCTCTTAATAGTGATATTGGCGGCA
GCAGTGCTAATGTTCCTGATCTGATGGATGAGTTTATAGCAGAACGACTT
CGAAGTGGTAATGCCTCGACTATGACAAGAAGAGGAAGTAGTCCAGGCAG
CCTGGAAATTCCCAAAGACCTCCCTGATATTCTAAACAAGCAGAACCAGA
TGCGCCCTATTGATGACCCAGGTGTGCCCTCAGAATGGACTTCTCCTGCC
AGTGCAGGGAGCAGTGATCTTATCAGCTCAGATAGTCATTCGGATTCTTT
CAGCGCTTTCCAATATGATGGCCGAAAATTTGACAATTTTGGCTTTGGAA
CCGACACTGGGGTTACGTCCTCTGCTGATGTGGATTCAGGTTCTGGCCAT
CATCAGAGTGCTGAAGAGCAGGAAGTGGCTAGTCTAACTACTCTTCATAT
AGATTCTGAAACAAGCAGTCTTAATCAGCAAGCTTTCTCTGCTGAAGTTG
CAACTATTACTGGTTCAGAAAGTGCTTCCCCAGTCCACTCACCTCTGGGC
TCCAGGTCACAGACTCCTTCCCCTTCTACATTGAATATAGATCACATGGA
ACAGAAGGATCTGCAGCTCGACGAGAAGCTCCACCACTCTGTTCTTCAGA
CACCAGATGATCTAGAAATTAGTGAATTTCCATCAGAATGTTGTAGTGTG
ATGGCAGGAGGTACTCTGACTGGATGGCATGCTGATGTTGCTACTGTAAT
GTGGCGAAGAATGCTAGGCATTTTGGGAGATGTAAATTCAATCATGGATC
CTGAAATACATGCTCAAGTTTTTGATTACCTCTGTGAACTTTGGCAGAAT
CTAGCTAAGATTAGAGATAACCTTGGCATTTCAACTGATAACCTGACCTC
CCCTTCTCCACCAGTTTTAATTCCTCCACTGAGAATTCTTACACCTTGGC
TTTTTAAGGCAACCATGTTGACTGATAAATATAAACAAGGTAAATTACAT
GCATATAAACTTATTTGTAATACAATGAAAAGAAGACAAGATGTTTCTCC
AAATAGAGATTTTCTAACACATTTCTAGCGGCCGC

Amino Acid Sequence

MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTLKFICT
TGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIF
FKDDGNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHN
VYIMADKQKNGIKVNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNH
YLSTQSALSKDPNEKRDHMVLLEFVTAAGITLGMDELYKSGLGSSRKGTI
DAAAMFSKKPHGDVKKSTQKVLDTKKDALTRLKHLRIVIENAESIDLKQF
FDQHFSHIYYVFFENFVTIEASLKQKGHKSQREELDAILFIFEKILQLLP
ERIHQRWQFHSIGLILKKLLHTGNSLKIRREGVRLFLLWLQALQNNCSKE
QLWMFSCLIPGFSAPQSEHGPRTLDNLINPPLNLQETQVTIEEITPLVPP
QSGDKGQEDLTSYFLEALLKYIVIQVKSLEWKNKENQERGFSFLFSHFKK
YYLPYIFPNICKENSLYHPILDIPQMRPKPHYVVIKKDAETNEAIYCTKE
PFIKARVIVIRWLVSFWLEPKPHTGPHIPGMEGEVLPKNIQRAAASLVSR
EESKNDNADKTDRTTEPEQSHSNTSTLTEREPSSSSLCSIDEEHLTDIEI
VRRVFSSKRSNVNFVTEIFRQAFLLPICEAAAMRKVVKVYQEWIQQEEKP
LFMQEPEEIVITSSDLPCIENVTDHDISMEEGEKREEENGTNTADHVRNS
SWAKNGSYQGALHNASEEATEQNIRAGTQAVLQVFIINSSNIFLLEPANE
IKNLLDEHTDMCKRILNIYRYMVVQVSMDKKTWEQMLLVLLRVTESVLKM
PSQAFLQFQGKKNMTLAGRLAGPLFQTLIVAWIKANLNVYISRELWDDLL
SVLSSLTYWEELATEWSLTMETLTKVLARNLYSLDLSDLPLDKLSEQKQK
KHKGKGVGHEFQKVSVDKSFSRGWSRDQPGQAPMRQRSATTTGSPGTEKA
RSIVRQKTVDIDDAQILPRSTRVRHFSQSEETGNEVFGALNEEQPLPRSS
STSDILEPFTVERAKVNKEDMSQKLPPLNSDIGGSSANVPDLMDEFIAER
LRSGNASTMTRRGSSPGSLEIPKDLPDILNKQNQMRPIDDPGVPSEWTSP
ASAGSSDLISSDSHSDSFSAFQYDGRKFDNFGFGTDTGVTSSADVDSGSG
HHQSAEEQEVASLTTLHIDSETSSLNQQAFSAEVATITGSESASPVHSPL
GSRSQTPSPSTLNIDHMEQKDLQLDEKLHHSVLQTPDDLEISEFPSECCS
VMAGGTLTGWHADVATVMWRRMLGILGDVNSIMDPEIHAQVFDYLCELWQ
NLAKIRDNLGISTDNLTSPSPPVLIPPLRILTPWLFKATMLTDKYKQGKL
HAYKLICNTMKRRQDVSPNRDFLTHF*

• Vector Type: Mammalian
• Antibiotic: Amp
• DU Number: DU16667
• Unit Source: Protein Phosphorylation and Ubiquitylation Unit
• Location: PPU
• MTA: Dundee