cDNA Clone MNK2a

• Synonyms: MKNK2, MNK2
• Plasmid: pGEX6P-MNK2a V2-S440
• Parent Plasmid: pGEX6P1
• Expressed: GST-MNK2a V2-S440
• Genbank: AF237775
• Tag: GST
• Species: Human

Sequence of Insert

ggatccGTGCAGAAGAAACCAGCCGAACTTCAGGGTTTCCACCGTTCGTTCAAGGGGCAGAACCCCTTCGAGCTGGCCTTCTCCCTAGACCAGCCCGACCACGGAGACTCTGACTTTGGCCTGCAGTGCTCAGCCCGCCCTGACATGCCCGCC
AGCCAGCCCATTGACATCCCGGACGCCAAGAAGAGGGGCAAGAAGAAGAAGCGCGGCCGGGCCACCGACAGCTTCTCGGGCAGGTTTGAAGACGTCTACCAGCTGCAGGAAGATGTGCTGGGGGAGGGCGCTCATGCCCGAGTGCAGACCTGCATCAACCTGATCACCAGCCAGGAGTACGCCGTCAAGATCATTGAGAAGCAGCCAGGCCACATTCGGAGCAGGGTTTTCAGGGAGGTGGAGATGCTGTACCAGTGCCAGGGACACAGGAACGTCCTAGAGCTGATTGAGTTCTTCGAGGAGGAGGACCGCTTCTACCTGGTGTTTGAGAAGATGCGGGGAGGCTCCATCCTGAGCCACATCCACAAGCGCCGGCACTTCAACGAGCTGGAGGCCAGCGTGGTGGTGCAGGACGTGGCCAGCGCCTTGGACTTTCTGCATAACAAAGGCATCGCCCACAGGGACCTAAAGCCGGAAAACATCCTCTGTGAGCACCCCAACCAGGTCTCCCCCGTGAAGATCTGTGACTTCGACCTGGGCAGCGGCATCAAACTCAACGGGGACTGCTCCCCTATCTCCACCCCGGAGCTGCTCACTCCGTGCGGCTCGGCGGAGTACATGGCCCCGGAGGTAGTGGAGGCCTTCAGCGAGGAGGCTAGCATCTACGACAAGCGCTGCGACCTGTGGAGCCTGGGCGTCATCTTGTATATCCTACTCAGCGGCTACCCGCCCTTCGTGGGCCGCTGTGGCAGCGACTGCGGCTGGGACCGCGGCGAGGCCTGCCCTGCCTGCCAGAACATGCTGTTTGAGAGCATCCAGGAGGGCAAGTACGAGTTCCCCGACAAGGACTGGGCCCACATCTCCTGCGCTGCCAAAGACCTCATCTCCAAGCTGCTGGTCCGTGACGCCAAGCAGAGGCTGAGTGCCGCCCAAGTCCTGCAGCACCCCTGGGTTCAGGGGTGCGCCCCGGAGAACACCTTGCCCACTCCCATGGTCCTGCAGAGGAACAGCTGTGCCAAAGACCTCACGTCCTTCGCGGCTGAGGCCATTGCCATGAACCGGCAGCTGGCCCAGCACGACGAGGACCTGGCTGAGGAGGAGGCCGCGGGGCAGGGCCAGCCCGTCCTGGTCCGAGCTACCTCACGCTGCCTGCAGCTGTCTCCACCCTCCTAGTCCAAGCTGGCGCAGCGGCGGCAAAGGGCCAGTCTGTCCTCGGCCCCAGTGGTCCTGGTGGGAGACCACGCCCACCATCACCATCACCATTGAgaattc

Amino Acid Sequence

MSPILGYWKIKGLVQPTRLLLEYLEEKYEEHLYERDEGDKWRNKKFELGL
EFPNLPYYIDGDVKLTQSMAIIRYIADKHNMLGGCPKERAEISMLEGAVL
DIRYGVSRIAYSKDFETLKVDFLSKLPEMLKMFEDRLCHKTYLNGDHVTH
PDFMLYDALDVVLYMDPMCLDAFPKLVCFKKRIEAIPQIDKYLKSSKYIA
WPLQGWQATFGGGDHPPKSDLEVLFQGPLGSVQKKPAELQGFHRSFKGQNPFELAFSLDQPDHGDSDFGLQCSARPDMPASQPIDIPDAKKRGKKKKRGRATDSFSGRFEDVYQLQEDVLGEGAHARVQTCINLITSQEYAVKIIEKQPGHIRSRVFREVEMLYQCQGHRNVLELIEFFEEEDRFYLVFEKMRGGSILSHIHKRRHFNELEASVVVQDVASALDFLHNKGIAHRDLKPENILCEHPNQVSPVKICDFDLGSGIKLNGDCSPISTPELLTPCGSAEYMAPEVVEAFSEEASIYDKRCDLWSLGVILYILLSGYPPFVGRCGSDCGWDRGEACPACQNMLFESIQEGKYEFPDKDWAHISCAAKDLISKLLVRDAKQRLSAAQVLQHPWVQGCAPENTLPTPMVLQRNSCAKDLTSFAAEAIAMNRQLAQHDEDLAEEEAAGQGQPVLVRATSRCLQLSPPS*

• Vector Type: Bacterial
• Antibiotic: Amp
• DU Number: DU16619
• Unit Source: Protein Phosphorylation and Ubiquitylation Unit
• Location: PPU
• MTA: Dundee
• Notes: Bacterial construct to express GST N-terminal fusion.; Method : Mutagenesis of pGEX6P-1 MNK2a 6his DU1214