cDNA Clone MNK1

• Synonyms: MKNK1
• Plasmid: pGEX6P-MNK1 V2-C399 (lacking inactivating insert)
• Parent Plasmid: pGEX6P1
• Expressed: GST-MNK1 V2-C399
• Genbank: AB000409 (lacks an inactivating 41 aa insertion in the catalytic domain that is present in some MNK1 mRNA sequences)
• Tag: GST
• Species: Human

Sequence of Insert

ggatccGTATCTTCTCAAAAGTTGGAAAAACCTATAGAGATGGGCAGTAG
CGAACCCCTTCCCATCGCAGATGGTGACAGGAGGAGGAAGAAGAAGCGGA
GGGGCCGGGCCACTGACTCCTTGCCAGGAAAGTTTGAAGATATGTACAAG
CTGACCTCTGAATTGCTTGGAGAGGGAGCCTATGCCAAAGTTCAAGGTGC
CGTGAGCCTACAGAATGGCAAAGAGTATGCCGTCAAAATCATCGAGAAAC
AAGCAGGGCACAGTCGGAGTAGGGTGTTTCGAGAGGTGGAGACGCTGTAT
CAGTGTCAGGGAAACAAGAACATTTTGGAGCTGATTGAGTTCTTTGAAGA
TGACACAAGGTTTTACTTGGTCTTTGAGAAATTGCAAGGAGGTTCCATCT
TAGCCCACATCCAGAAGCAAAAGCACTTCAATGAGCGAGAAGCCAGCCGA
GTGGTGCGGGACGTTGCTGCTGCCCTTGACTTCCTGCATACCAAAGGCAT
TGCTCATCGTGATCTGAAACCAGAAAATATATTGTGTGAATCTCCAGAAA
AGGTGTCTCCAGTGAAAATCTGTGACTTTGACTTGGGCAGTGGGATGAAA
CTGAACAACTCCTGTACCCCCATAACCACACCAGAGCTGACCACCCCATG
TGGCTCTGCAGAATACATGGCCCCTGAGGTAGTGGAGGTCTTCACGGACC
AGGCCACATTCTACGACAAGCGCTGTGACCTGTGGAGCCTGGGCGTGGTC
CTCTACATCATGCTGAGTGGCTACCCACCCTTCGTGGGTCACTGCGGGGC
CGACTGTGGCTGGGACCGGGGCGAGGTCTGCAGGGTGTGCCAGAACAAGC
TGTTTGAAAGCATCCAGGAAGGCAAGTATGAGTTTCCTGACAAGGACTGG
GCACACATCTCCAGTGAAGCCAAAGACCTCATCTCCAAGCTCCTGGTGCG
AGATGCAAAGCAGAGACTTAGCGCCGCCCAAGTTCTGCAGCACCCATGGG
TGCAGGGGCAAGCTCCAGAAAAGGGACTCCCCACGCCGCAAGTCCTCCAG
AGGAACAGCAGCACAATGGACCTGACGCTCTTCGCAGCTGAGGCCATCGC
CCTTAACCGCCAGCTATCTCAGCACGAAGAGAACGAACTAGCAGAGGAGC
CAGAGGCACTAGCTGATGGCCTCTGCTCCATGAAGCTTTCCCCTCCCTGC
TAGTCACGCCTGGCCCGGAGACGGGCCCTGGCCCAGGCAGGCCGTGGTGA
AGACAGGAGCCCGCCCACAGCACTCCATCACCATCACCATCACTGAgaattc

Amino Acid Sequence

MSPILGYWKIKGLVQPTRLLLEYLEEKYEEHLYERDEGDKWRNKKFELGL
EFPNLPYYIDGDVKLTQSMAIIRYIADKHNMLGGCPKERAEISMLEGAVL
DIRYGVSRIAYSKDFETLKVDFLSKLPEMLKMFEDRLCHKTYLNGDHVTH
PDFMLYDALDVVLYMDPMCLDAFPKLVCFKKRIEAIPQIDKYLKSSKYIA
WPLQGWQATFGGGDHPPKSDLEVLFQGPLGSVSSQKLEKPIEMGSSEPLP
IADGDRRRKKKRRGRATDSLPGKFEDMYKLTSELLGEGAYAKVQGAVSLQ
NGKEYAVKIIEKQAGHSRSRVFREVETLYQCQGNKNILELIEFFEDDTRF
YLVFEKLQGGSILAHIQKQKHFNEREASRVVRDVAAALDFLHTKGIAHRD
LKPENILCESPEKVSPVKICDFDLGSGMKLNNSCTPITTPELTTPCGSAE
YMAPEVVEVFTDQATFYDKRCDLWSLGVVLYIMLSGYPPFVGHCGADCGW
DRGEVCRVCQNKLFESIQEGKYEFPDKDWAHISSEAKDLISKLLVRDAKQ
RLSAAQVLQHPWVQGQAPEKGLPTPQVLQRNSSTMDLTLFAAEAIALNRQ
LSQHEENELAEEPEALADGLCSMKLSPPC*

• Vector Type: Bacterial
• Antibiotic: Amp
• DU Number: DU16618
• Unit Source: Protein Phosphorylation and Ubiquitylation Unit
• Location: PPU
• MTA: Dundee
• Notes: Bacterial construct to express MNK1 V2-C399 with N-terminal GST.; Method : Mutagenesis of pGEX6P-1 MNK1.6his DU1385