cDNA Clone RMI1

• Plasmid: pEBG6P-1 RMI1 (455=N: matches NM_024945)
• Parent Plasmid: pEBG6P
• Expressed: GST-RMI1
• Genbank: NM_024945.2 GI:153791760
• Tag: GST
• Species: Human

Sequence of Insert

GCGGCCGCAATGAATGTGACTAGTATTGCATTAAGAGCTGAAACTTGGCT
TTTAGCTGCATGGCATGTTAAAGTACCTCCGATGTGGCTGGAAGCTTGTA
TTAACTGGATTCAAGAAGAAAATAATAATGTTAACTTGAGTCAGGCCCAA
ATGAATAAACAAGTGTTTGAGCAGTGGCTCCTTACTGATCTGAGGGATTT
GGAGCATCCTCTTTTACCCGATGGCATTTTAGAAATTCCAAAAGGAGAAT
TAAATGGATTTTATGCTCTGCAGATTAATTCCTTGGTTGATGTAAGTCAG
CCTGCATACTCCCAGATACAGAAGTTGAGAGGAAAGAATACAACAAATGA
TCTAGTTACAGCTGAAGCACAAGTAACCCCAAAACCTTGGGAAGCAAAGC
CTTCACGAATGTTGATGCTGCAGCTAACTGATGGAATCGTACAAATACAG
GGAATGGAATATCAGCCTATTCCAATTCTTCATAGTGATCTTCCTCCAGG
TACAAAAATTTTGATTTATGGAAATATATCTTTCCGTCTTGGTGTTCTCT
TATTGAAACCAGAAAACGTGAAAGTGTTAGGAGGTGAAGTAGATGCTCTT
TTAGAAGAATATGCCCAAGAAAAAGTACTTGCAAGATTAATAGGGGAACC
TGATCTTGTAGTTTCAGTCATACCAAACAATTCTAACGAAAACATTCCCA
GAGTTACAGATGTTCTAGATCCTGCATTAGGTCCTTCTGATGAAGAACTC
TTGGCAAGTCTTGATGAAAATGATGAGCTTACAGCAAATAATGACACTTC
CTCAGAACGATGTTTCACCACAGGTAGTTCCTCAAATACCATTCCCACAA
GACAGTCAAGTTTTGAGCCAGAATTTGTTATTTCTCCAAGACCAAAAGAG
GAACCATCAAACCTATCTATACATGTAATGGATGGAGAATTAGATGACTT
TTCACTGGAGGAGGCCTTGCTTTTAGAAGAAACTGTCCAGAAAGAACAGA
TGGAAACTAAGGAATTGCAACCATTGACTTTTAACAGAAATGCCGATCGA
AGTATAGAGAGATTTTCACATAATCCTAATACTACGAATAACTTTTCTTT
GACTTGCAAAAATGGAAACAATAATTGGAGTGAAAAAAATGTATCTGAAC
AAATGACTAATGAAGACAAATCATTTGGTTGTCCATCTGTTAGAGACCAA
AACAGGAGTATTTTTTCAGTTCATTGTAATGTACCCTTAGCCCATGATTT
TACAAATAAAGAAAAGAACTTAGAGACAGATAATAAAATAAAACAAACCA
GCAGTTCAGATAGCCATTCCTTAAATAATAAAATATTAAATAGAGAGGTG
GTCAACTATGTACAGAAAAGGAATTCACAAATTTCTAATGAAAATGATTG
TAATTTACAGAGTTGTTCTTTAAGATCATCAGAGAATAGCATTAATCTTT
CTATTGCCATGGATTTGTATTCTCCACCCTTTGTCTATTTGTCTGTTCTA
ATGGCCAGCAAACCAAAGGAAGTTACAACAGTGAAAGTGAAAGCATTTAT
TGTAACCTTAACTGGAAATCTCTCAAGTTCTGGTGGTATTTGGAGTATAA
CTGCAAAGGTGTCTGATGGTACTGCATATCTAGATGTAGACTTTGTGGAT
GAAATACTTACTAGCTTGATAGGGTTCTCAGTACCAGAAATGAAACAGTC
AAAAAAGGATCCTCTTCAATACCAAAAGTTCCTGGAAGGGTTGCAGAAAT
GTCAAAGAGATCTAATAGATTTGTGCTGTCTAATGACTATTTCATTTAAT
CCTTCCTTGTCTAAAGCAATGGTACTGGCATTACAAGATGTTAATATGGA
ACACCTTGAGAATCTAAAGAAGCGGTTAAATAAATAAGCGGCCGC

Amino Acid Sequence

MAPILGYWKIKGLVQPTRLLLEYLEEKYEEHLYERDEGDKWRNKKFELGL
EFPNLPYYIDGDVKLTQSMAIIRYIADKHNMLGGCPKERAEISMLEGAVL
DIRYGVSRIAYSKDFETLKVDFLSKLPEMLKMFEDRLCHKTYLNGDHVTH
PDFMLYDALDVVLYMDPMCLDAFPKLVCFKKRIEAIPQIDKYLKSSKYIA
WPLQGWQATFGGGDHPPKSDLVPRGSLEVLFQGPLGSTSGTIDAAAMNVT
SIALRAETWLLAAWHVKVPPMWLEACINWIQEENNNVNLSQAQMNKQVFE
QWLLTDLRDLEHPLLPDGILEIPKGELNGFYALQINSLVDVSQPAYSQIQ
KLRGKNTTNDLVTAEAQVTPKPWEAKPSRMLMLQLTDGIVQIQGMEYQPI
PILHSDLPPGTKILIYGNISFRLGVLLLKPENVKVLGGEVDALLEEYAQE
KVLARLIGEPDLVVSVIPNNSNENIPRVTDVLDPALGPSDEELLASLDEN
DELTANNDTSSERCFTTGSSSNTIPTRQSSFEPEFVISPRPKEEPSNLSI
HVMDGELDDFSLEEALLLEETVQKEQMETKELQPLTFNRNADRSIERFSH
NPNTTNNFSLTCKNGNNNWSEKNVSEQMTNEDKSFGCPSVRDQNRSIFSV
HCNVPLAHDFTNKEKNLETDNKIKQTSSSDSHSLNNKILNREVVNYVQKR
NSQISNENDCNLQSCSLRSSENSINLSIAMDLYSPPFVYLSVLMASKPKE
VTTVKVKAFIVTLTGNLSSSGGIWSITAKVSDGTAYLDVDFVDEILTSLI
GFSVPEMKQSKKDPLQYQKFLEGLQKCQRDLIDLCCLMTISFNPSLSKAM
VLALQDVNMEHLENLKKRLNK*

• Vector Type: Mammalian
• Antibiotic: Amp
• DU Number: DU16248
• Unit Source: Protein Phosphorylation and Ubiquitylation Unit
• Location: PPU
• MTA: Dundee
• Notes: Mammalian expression plasmid to express RMI1 with N-terminal GST (contains preScission protease site); ; Estimated MW of expressed protein : 98347 Daltons