cDNA Clone RALGAPA1-S (mouse)

• Synonyms: Garnl1, GRIPE, Tulip1
• Plasmid: pcDNA5-FRT/TO-HA RALGAPA1-S (mouse) S859A
• Parent Plasmid: pcDNA5 FRT/TO GFP
• Expressed: HA-RALGAPA1-S (mouse) S859A
• Genbank: NM_019994.4 GI:51230428 (with a3400g (T1134A) (Agreed with Shuai Chen to leave in 08/08/11))
• Tag: HA
• Species: Mouse

Sequence of Insert

agatctatgttctctaagaagccgcacggggacgtgaagaagtccacccagaaggtgctggacaccaagaaggacgcgctgacccgcctcaagcacctgcgcatcgtcatcgagaatgcagaatctattgaccttaaacagttttttgaccaacatttttcacatatatactatgtgttttttgaaaattttgtgactattgaagctagtcttaaacagaaaggtcacaagtctcaaagagaggaattagatgctatactttttatttttgagaaaattttacaacttcttccagaaagaattcatcagcgatggcaattccatagtattgggttgattttaaagaaactactgcacacaggaaactccttaaagatcagacgggaaggtgttcgtctcttcttgttatggttgcaagctcttcaggataactgcagcaaagaacagctctggatgttttcatgcttgatccctggattctcagcaccacaatccgaatatggacctcggactttagataatcttattaatcctccactcagccttcaagaaactcaagtaactatagaggaagtcactcctcttgttccgccacagtcaggagataaaggacaagaagacctcacaagctattttcttgaagcacttttaaaatacatagtaattcaggtaaaaagtttagaatggaagaacaaagaaaaccaagaaaggggattttcatttttgttttcacattttaagaagttttacttgccttatattttcccaaacatttgcaaggagaatagtttgtatcatcctgtacttgatatcccacagatcagaccaaagcctcattatgtgatgataaagaaggatgctgaaaccaatgaaacaatatattgtacaaaagagcctttcatccaggctcgggttattgtcattcgttggctggtttctttctggctggagccaaaaccacattcaggacctaatattcctgggatggaaggtgaagtcttgccaaagaatattcagagagcagctgctagtttggtttccagagaagaaagcaaaaatgatactgttgataaagtagacaagtctgcagagcctgagcagtctcattccaacacaagcactctcacggagcgagagcctagctcttccagtctctgcagcatcgacgaagaacatctcacagacatcgaaattgttcgcagagtgttttcatctaagagaagcaatgtaaactttgtcacagagatatttcgtcaggcatttttattaccaatttgtgaagcagcagctatgagaaaagtggtgaaagtgtatcaagaatggattcaacaagaggaaaagcctttgtttatgcaagagcctgaggacactgcgatcacttgttcagatataccctgcagcgaaactgttgcagaccatgacagtgcaatagaagatggagagaaaagagaagaggaaaatgggaccagtacttctgaacatgttcgcaattctagctggacaaaaaatggttcctaccaagaagcttttcatgtctgtgaagaagccacagaacagaatattcaagctggcactcaggccgttttgcaggtgtttattataaactcatcgaacatatttcttcttgaacctgcaaatgaaataaagaatcttctggatgagcatacagatatgtgtaaacgcattcttaatatttatcgatacatggttgtgcaagtatcaatggacaaaaagacctgggaacagatgctgctcgtgctgctcagagtcacggagtccgtactgaagatgtcgtcacaagcttttctgcagttccaggggaaaaaaagcatgaccctggcgggtcgacttgcaggaccactttttcagactctcattgttgcctggatcaaagcaaatctaaatgtatacatttctcgagaactttgggatgacttactttcggtattgtcatcattgacctattgggaagaattggccactgagtggtcactgaccatggagacactgactaaagttttggctagaaatttatatagtttggatctcagtgatttgccattggataagctcagtgaacagaaacaaaaaaagcacaaagggaaaggagttggacacgaatttcagaaagtttcagttgacaagtcattttctagaggatggagtcgcgatcagcctggccaggcacctatgagacagagaagtgcaacgaccactggctccccaggaactgagaaggccaggagtatagtacggcaaaaaactgttgatattgatgatgctcaaatccttccccgctcaaccagagtcagacatttttcacagagtgaagacactggaaatgaagtttttggtgctttgcatgaggaacagccactgcctcgaagtagcagcacttctgacatcttggaaccattcactgttgagcgagccaaagtaaataaagaggacacgagccccaaactgcctcctcttaatagtgagactggtggcaacagcgccaatgttcctgacctgatggatgagtttatcgcagagcgacttcgaagtggcaatgcctcgactatgacaagaaggggggctagtccaggcagccttgaaatcccgaaagacctccctgatattctaaacaagcagaaccagatgcggcctgttgatgaccctggtgtaccctccgaatggacgtctccagccagtgcagggagcagtgatctcatgagctcagatagtcactcggattccttcagtgccttccagtgtgagggacgaaaatttgacaactttggcttcgggactgacattgggatcccatcttctgctgatgtggatttaggttctggccatcatcaaagcactgaggagcaggaagtggccagcctaactacgcttcatttagattcagaaacaagcagccttaatcagcaagccttctctgcagaagttgcaaccgttactggttcagaaagtgcttcccccgttcattcagctctgggatccaggtcgcagaccccctccccatctacactgagcagagctcacatagaacagaaggacctacagctcgatgagaagctccaccactctgttcttcagacgccggatgacctagaaatcagtgaatttccatctgaatgttgtagcgtgatggcaggaggtactctcactgggtggcatgctgatgttgctactgttatgtggcgcagaatgctgggcattttgggagatgtcaatgctattatggatcctgaaatacatgctcaagtttttgattacctctgtgaactttggcagaatctagccaagatcagagataaccttggcatttcagctgacaacctgacttccccttctccacccgtcctgattcctccactaagaattcttacaccttggctttttaaggcagccatgttgactgataagtataaacaaggtaaattacatgcatataaacttatttgtaatacaatgaagagaagacaagatgtatctccaaatagagattttttaacacatttctacaatataatgcattgtggattgcttcatattgaccaggacattgtcaatacgatcatcaagcactgctcacctcagtttttttcactcggtttgcctggggccacgatgcttattatggattttattatagcagctgggagagtggcttcttcggcttttcttaatgcaccaagagtggaagcacaagttcttctgggatctttagtttgctttcccaacttatattgtgaattgcctgctctccatcccaacattcctgatattgctgtgtctcaatttacagatgttaaggaacttataattaaaactgtattaagctcggcaagagatgagccctctggtcctgcacgatgtgtagcactttgcagtttaggtatttggatttgtgaagaattagtccacgaatctcatcatcctcaaattaaagaagcactgaatgtaatttgtgtttcattaaagtttactaataaaacagttgcccacgtcgcctgtaacatgcttcacatgctggttcactatgtccctagacttcagattcaccagccgcagtctcccctgaaaattattcagatcctaatagcaactatcacccaccttttgccaagtacagaagcttcatcatatgaaatggacaagaggttggttgtctcattacttctttgccttctggactggatcatggccttacctttaaagacactgctccaaccagtccatgctacaggagcagaaaatgataagaccgaaaagtctgttctcaactgcatttataaggtattacatgggtgtgtctatggagcgcaaagttttagcaatcccaagtactttccaataagcctttctgatttggcatctgtagattatgatccgtttatgcatttggaaagtctgaaagaacctgaacctttacactctccagattcagagcggtcttctaaactgcagcctgtgacagaagtgaaaactcagatgcaacaaggattaatctctatagcagcccgtactgttattacccatctggtgaatcacttgggccattatccaatgagtggtggtcctgctatgctaacaagtcaggtgtgtgaaaatcatgacaatcattacagtgaaagtactgaactttctcctgagctctttgaaagtccaaacatccagttctttgtgctgaataatacaaccttagtgtcctgtatccagataagatcggaagagagtatgcctggaggaggtctagctgctggccttgtgtcagccaactcaaatgttagaatcatagtacgtgatctctctggaaagtactcatgggattctgccatactgtatggaccacccattgtaagtggtttgcctgaacctacatctttcatactttcaatgtctgaccaagagaaaccggaagagcctcctacatctaacgaatgcttagaagacatcgcagtaaaagatgggctttctctccagcttagaagatttagagaaactgtaccaacttggtctacgatacgagaggaagaagatgttcttgacgagctcctgcagtatttaggcactacaagtcctgagtgtttacagagaacgggaatctccctcaatgttcctgctccacagcctctgtgcatttctgaaaaacaggagaatgatgtcattaatgctatccttaagcaatacacggaagaaaaagagtttgtagagaagcactttaatgacttaaacatgaaagcctcagaacaagatgagccaacacctcagaaacctcagtcagctttttattactgcagattgcttcttagtatattgggaatgaattcctgggacaaacggaggagctttcatctcttgaagaaaaatgaaaagctgctcagagaacttaggaacttggattcaaggcaatgccgagagacacacaagattgcagtattttacgttgctgaaggacaagaagataaatactccattctcaccaatataggaggaagccaagcatatgaagattttgtagctggccttggttgggaggtaaaccttacaaaccactgtggcttcatgggaggccttcagaagaacagaagcactggactgaccactccctattttgctacctcgacagtagaagtaatatttcatgtatctacacgaatgccttctgattctgatgactctctgaccaaaaaattgagacatctaggaaatgatgaagtgcacattgtttggtcagaacatactagagactacaggagaggaattattcctactgagtttggtgatgtcctcattgtaatatacccaatgaaaaaccacatgttcagtatccagatcatgaaaaaaccagaggttcccttctttggtccactttttgatggtgctattgtgaatggaaaagttctgcccattatggttcgatccacagcaataaatgctagccgtgctctgaaatcactgattccattgtatcaaaatttctatgaggagagagcacggtacctgcagacaattgtccagcaccatttagagccaacaacatttgaagactttgcagcacaggttttctctccagctccctaccatcatttccctgctgacgcagatcattaagcggccgc

Amino Acid Sequence

MYPYDVPDYAGSMFSKKPHGDVKKSTQKVLDTKKDALTRLKHLRIVIENAESIDLKQFFDQHFSHIYYVFFENFVTIEASLKQKGHKSQREELDAILFIFEKILQLLPERIHQRWQFHSIGLILKKLLHTGNSLKIRREGVRLFLLWLQALQDNCSKEQLWMFSCLIPGFSAPQSEYGPRTLDNLINPPLSLQETQVTIEEVTPLVPPQSGDKGQEDLTSYFLEALLKYIVIQVKSLEWKNKENQERGFSFLFSHFKKFYLPYIFPNICKENSLYHPVLDIPQIRPKPHYVMIKKDAETNETIYCTKEPFIQARVIVIRWLVSFWLEPKPHSGPNIPGMEGEVLPKNIQRAAASLVSREESKNDTVDKVDKSAEPEQSHSNTSTLTEREPSSSSLCSIDEEHLTDIEIVRRVFSSKRSNVNFVTEIFRQAFLLPICEAAAMRKVVKVYQEWIQQEEKPLFMQEPEDTAITCSDIPCSETVADHDSAIEDGEKREEENGTSTSEHVRNSSWTKNGSYQEAFHVCEEATEQNIQAGTQAVLQVFIINSSNIFLLEPANEIKNLLDEHTDMCKRILNIYRYMVVQVSMDKKTWEQMLLVLLRVTESVLKMSSQAFLQFQGKKSMTLAGRLAGPLFQTLIVAWIKANLNVYISRELWDDLLSVLSSLTYWEELATEWSLTMETLTKVLARNLYSLDLSDLPLDKLSEQKQKKHKGKGVGHEFQKVSVDKSFSRGWSRDQPGQAPMRQRSATTTGSPGTEKARSIVRQKTVDIDDAQILPRSTRVRHFSQSEDTGNEVFGALHEEQPLPRSSSTSDILEPFTVERAKVNKEDTSPKLPPLNSETGGNSANVPDLMDEFIAERLRSGNASTMTRRGASPGSLEIPKDLPDILNKQNQMRPVDDPGVPSEWTSPASAGSSDLMSSDSHSDSFSAFQCEGRKFDNFGFGTDIGIPSSADVDLGSGHHQSTEEQEVASLTTLHLDSETSSLNQQAFSAEVATVTGSESASPVHSALGSRSQTPSPSTLSRAHIEQKDLQLDEKLHHSVLQTPDDLEISEFPSECCSVMAGGTLTGWHADVATVMWRRMLGILGDVNAIMDPEIHAQVFDYLCELWQNLAKIRDNLGISADNLTSPSPPVLIPPLRILTPWLFKAAMLTDKYKQGKLHAYKLICNTMKRRQDVSPNRDFLTHFYNIMHCGLLHIDQDIVNTIIKHCSPQFFSLGLPGATMLIMDFIIAAGRVASSAFLNAPRVEAQVLLGSLVCFPNLYCELPALHPNIPDIAVSQFTDVKELIIKTVLSSARDEPSGPARCVALCSLGIWICEELVHESHHPQIKEALNVICVSLKFTNKTVAHVACNMLHMLVHYVPRLQIHQPQSPLKIIQILIATITHLLPSTEASSYEMDKRLVVSLLLCLLDWIMALPLKTLLQPVHATGAENDKTEKSVLNCIYKVLHGCVYGAQSFSNPKYFPISLSDLASVDYDPFMHLESLKEPEPLHSPDSERSSKLQPVTEVKTQMQQGLISIAARTVITHLVNHLGHYPMSGGPAMLTSQVCENHDNHYSESTELSPELFESPNIQFFVLNNTTLVSCIQIRSEESMPGGGLAAGLVSANSNVRIIVRDLSGKYSWDSAILYGPPIVSGLPEPTSFILSMSDQEKPEEPPTSNECLEDIAVKDGLSLQLRRFRETVPTWSTIREEEDVLDELLQYLGTTSPECLQRTGISLNVPAPQPLCISEKQENDVINAILKQYTEEKEFVEKHFNDLNMKASEQDEPTPQKPQSAFYYCRLLLSILGMNSWDKRRSFHLLKKNEKLLRELRNLDSRQCRETHKIAVFYVAEGQEDKYSILTNIGGSQAYEDFVAGLGWEVNLTNHCGFMGGLQKNRSTGLTTPYFATSTVEVIFHVSTRMPSDSDDSLTKKLRHLGNDEVHIVWSEHTRDYRRGIIPTEFGDVLIVIYPMKNHMFSIQIMKKPEVPFFGPLFDGAIVNGKVLPIMVRSTAINASRALKSLIPLYQNFYEERARYLQTIVQHHLEPTTFEDFAAQVFSPAPYHHFPADADH*

• Vector Type: Mammalian
• Antibiotic: Amp
• DU Number: DU36975
• Unit Source: Protein Phosphorylation and Ubiquitylation Unit
• Location: PPU
• MTA: Dundee
• Notes: Plasmid requires to be grown at low temp to avoid rearrangement