cDNA Clone UBA7

• Plasmid: pEFIRES-P-eYFP C1-UBA7 t2535a, c2541a, t2547c
• Parent Plasmid: pEFIRES puro eYFPC1
• Expressed: eYFP-UBA7 t2535a, c2541a, t2547c
• Genbank: NP_003326.2
• Tag: YFP
• Species: Human

Sequence of Insert

AGATCTATGGATGCCCTGGACGCTTCGAAGCTACTGGATGAGGAGCTGTA
TTCAAGACAGCTGTATGTGCTGGGCTCACCTGCCATGCAGAGGATTCAGG
GAGCCAGGGTCCTGGTGTCAGGCCTGCAGGGCCTGGGGGCCGAGGTGGCC
AAGAACTTGGTTCTGATGGGTGTGGGCAGCCTCACTCTGCATGATCCCCA
CCCCACCTGCTGGTCCGACCTGGCTGCCCAGTTTCTCCTCTCAGAGCAGG
ACTTGGAAAGGAGCAGAGCCGAGGCCTCTCAAGAGCTCTTGGCTCAGCTC
AACAGAGCTGTCCAGGTCGTCGTGCACACGGGTGACATCACTGAGGACCT
GCTGTTGGACTTCCAGGTGGTGGTGCTGACTGCTGCAAAGCTGGAGGAGC
AGCTGAAGGTGGGCACCTTGTGTCATAAGCATGGAGTTTGCTTTCTGGCG
GCTGACACCCGGGGCCTCGTGGGGCAGTTGTTCTGTGACTTTGGTGAGGA
CTTCACTGTGCAGGACCCCACAGAGGCAGAACCCCTGACAGCTGCCATCC
AGCACATCTCCCAGGGCTCCCCTGGCATTCTCACTCTGAGGAAAGGGGCC
AATACCCACTACTTCCGTGATGGAGACTTGGTGACTTTCTCGGGAATTGA
GGGAATGGTTGAGCTCAACGACTGTGATCCCCGGTCTATCCACGTGCGGG
AGGATGGGTCCCTGGAGATTGGAGACACAACAACTTTCTCTCGGTACTTG
CGTGGTGGGGCTATCACTGAAGTCAAGAGACCCAAGACTGTGAGACATAA
GTCCCTGGACACAGCCCTGCTCCAGCCCCATGTGGTGGCCCAGAGCTCCC
AGGAAGTTCACCATGCCCACTGCCTGCATCAGGCCTTCTGTGCACTGCAC
AAGTTCCAGCACCTCCATGGCCGGCCACCCCAGCCCTGGGATCCTGTTGA
TGCAGAGACTGTGGTGGGCCTGGCCCGGGACCTGGAACCACTGAAGCGGA
CAGAGGAAGAGCCACTGGAAGAGCCACTGGATGAGGCCCTAGTGCGGACA
GTCGCCCTAAGCAGTGCAGGTGTCTTGAGCCCTATGGTGGCCATGCTGGG
TGCAGTAGCTGCCCAGGAAGTGCTGAAGGCAATCTCCAGGAAGTTCATGC
CTCTGGACCAGTGGCTTTACTTTGATGCCCTCGATTGTCTTCCGGAAGAT
GGGGAGCTCCTTCCCAGTCCTGAGGACTGTGCCCTGAGAGGCAGCCGCTA
TGATGGGCAAATTGCAGTGTTTGGGGCTGGTTTTCAGGAGAAACTGAGAC
GCCAGCACTACCTCCTGGTGGGCGCTGGTGCCATTGGTTGTGAGCTGCTC
AAAGTCTTTGCCCTAGTGGGACTGGGGGCCGGGAACAGCGGGGGCTTGAC
TGTTGTTGACATGGACCACATAGAGCGCTCCAATCTCAGCCGTCAGTTCC
TCTTCAGGTCCCAGGACGTTGGTAGACCCAAGGCAGAGGTGGCTGCAGCA
GCTGCCCGGGGCCTGAACCCAGACTTACAGGTGATCCCGCTCACCTACCC
ACTGGATCCCACCACAGAGCACATCTATGGGGATAACTTTTTCTCCCGTG
TGGATGGTGTGGCTGCTGCCCTGGACAGTTTCCAGGCCCGGCGCTATGTG
GCTGCTCGTTGCACCCACTATCTGAAGCCACTGCTGGAGGCAGGCACATC
GGGCACCTGGGGCAGTGCTACAGTATTCATGCCACATGTGACTGAGGCCT
ACAGAGCCCCTGCCTCAGCTGCAGCTTCTGAGGATGCCCCCTACCCTGTC
TGTACCGTGCGGTACTTCCCTAGCACAGCCGAGCACACCCTGCAGTGGGC
CCGGCATGAGTTTGAAGAACTCTTCCGACTGTCTGCAGAGACCATCAACC
ACCACCAACAGGCACACACTTCCCTGGCAGACATGGATGAGCCACAGACA
CTCACCTTACTGAAGCCAGTGCTTGGGGTCCTGAGAGTGCGTCCACAGAA
CTGGCAAGACTGTGTGGCGTGGGCTCTTGGCCACTGGAAACTCTGCTTTC
ATTATGGCATCAAACAGCTGCTGAGGCACTTCCCACCTAATAAAGTGCTT
GAGGATGGAACTCCCTTCTGGTCAGGTCCCAAACAGTGTCCCCAGCCCTT
GGAGTTTGACACCAACCAAGACACACACCTCCTCTACGTACTGGCAGCTG
CCAACCTGTATGCCCAGATGCATGGGCTGCCTGGCTCACAGGACTGGACT
GCACTCAGGGAGCTGCTGAAGCTGCTGCCACAGCCTGACCCCCAACAGAT
GGCCCCCATCTTTGCTAGTAATCTAGAGCTGGCTTCGGCTTCTGCTGAGT
TTGGCCCTGAGCAGCAGAAGGAACTGAACAAAGCCCTGGAAGTCTGGAGT
GTGGGCCCTCCCCTGAAGCCTCTGATGTTTGAGAAGGATGATGACAGCAA
CTTCCATGTGGACTTTGTGGTAGCGGCAGCTAGCCTGAGATGTCAGAACT
ACGGGATTCCACCGGTCAACCGTGCCCAGAGCAAGCGAATAGTGGGACAG
ATCATCCCAGCCATTGCCACCACTACAGCAGCTGTGGCAGGCCTGTTGGG
CCTGGAGCTGTATAAGGTGGTGAGTGGGCCACGGCCTCGTAGTGCCTTTC
GCCACAGCTACCTACATCTGGCTGAAAACTACCTCATCCGCTATATGCCT
TTTGCCCCAGCCATCCAGACGTTCCATCACCTGAAGTGGACCTCTTGGGA
CCGTCTGAAGGTACCAGCTGGGCAGCCTGAGAGGACCCTGGAGTCGCTGC
TGGCTCATCTTCAGGAGCAGCACGGGTTGAGGGTGAGGATCCTGCTGCAC
GGCTCAGCCCTGCTCTATGCGGCCGGATGGTCACCTGAAAAGCAGGCCCA
GCACCTGCCCCTCAGGGTGACAGAACTGGTTCAGCAGCTGACAGGCCAGG
CACCTGCTCCTGGGCAGCGGGTGTTGGTGCTAGAGCTGAGCTGTGAGGGT
GACGACGAGGACACTGCCTTCCCACCTCTGCACTATGAGCTGTGAGCGGC
CGC

Amino Acid Sequence

MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTLKFICT
TGKLPVPWPTLVTTFGYGLQCFARYPDHMKQHDFFKSAMPEGYVQERTIF
FKDDGNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHN
VYIMADKQKNGIKVNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNH
YLSYQSALSKDPNEKRDHMVLLEFVTAAGITLGMDELYKSGLRSMDALDA
SKLLDEELYSRQLYVLGSPAMQRIQGARVLVSGLQGLGAEVAKNLVLMGV
GSLTLHDPHPTCWSDLAAQFLLSEQDLERSRAEASQELLAQLNRAVQVVV
HTGDITEDLLLDFQVVVLTAAKLEEQLKVGTLCHKHGVCFLAADTRGLVG
QLFCDFGEDFTVQDPTEAEPLTAAIQHISQGSPGILTLRKGANTHYFRDG
DLVTFSGIEGMVELNDCDPRSIHVREDGSLEIGDTTTFSRYLRGGAITEV
KRPKTVRHKSLDTALLQPHVVAQSSQEVHHAHCLHQAFCALHKFQHLHGR
PPQPWDPVDAETVVGLARDLEPLKRTEEEPLEEPLDEALVRTVALSSAGV
LSPMVAMLGAVAAQEVLKAISRKFMPLDQWLYFDALDCLPEDGELLPSPE
DCALRGSRYDGQIAVFGAGFQEKLRRQHYLLVGAGAIGCELLKVFALVGL
GAGNSGGLTVVDMDHIERSNLSRQFLFRSQDVGRPKAEVAAAAARGLNPD
LQVIPLTYPLDPTTEHIYGDNFFSRVDGVAAALDSFQARRYVAARCTHYL
KPLLEAGTSGTWGSATVFMPHVTEAYRAPASAAASEDAPYPVCTVRYFPS
TAEHTLQWARHEFEELFRLSAETINHHQQAHTSLADMDEPQTLTLLKPVL
GVLRVRPQNWQDCVAWALGHWKLCFHYGIKQLLRHFPPNKVLEDGTPFWS
GPKQCPQPLEFDTNQDTHLLYVLAAANLYAQMHGLPGSQDWTALRELLKL
LPQPDPQQMAPIFASNLELASASAEFGPEQQKELNKALEVWSVGPPLKPL
MFEKDDDSNFHVDFVVAAASLRCQNYGIPPVNRAQSKRIVGQIIPAIATT
TAAVAGLLGLELYKVVSGPRPRSAFRHSYLHLAENYLIRYMPFAPAIQTF
HHLKWTSWDRLKVPAGQPERTLESLLAHLQEQHGLRVRILLHGSALLYAA
GWSPEKQAQHLPLRVTELVQQLTGQAPAPGQRVLVLELSCEGDDEDTAFP
PLHYEL*

• Vector Type: Mammalian
• Antibiotic: Amp
• DU Number: DU20626
• Unit Source: Protein Phosphorylation and Ubiquitylation Unit
• Location: PPU
• MTA: Dundee
• Notes: Mammalian expression vector for eYFP- UBA7 t2535a, c2541a, t2547c. EF1a promoter, IRES and puromycin selection cassette; Parental Plasmid SC20522; Estimated MW of expressed protein : 139170 Daltons