cDNA Clone AATYK NCBI AY236858

• Synonyms: AATK
• Plasmid: pCMV-FLAG AATYK D254/V+D272/AA, R1327,37AA
• Parent Plasmid: pCMV5 FLAG1
• Expressed: FLAG-AATYK D254/V+D272/AA, R1327,37AA
• Genbank: AY236858
• Tag: FLAG

Sequence of Insert

AGATCTATGTCGTCGTCCTTCTTCAACCCCAGCTTTGCCTTCAGCTCCCACTTCGACCCGGACGGTGCCCCGCTCAGTGAGCTGTCCTGGTCCTCGTCCCTCGCGGTGGTAGCCGTGTCCTTCTCTGGAATCTTCACTGTCGTCATCCTCATGCTGGCCTGCCTGTGTTGTAAGAAGGGCGGCATCGGGTTCAAGGAGTTTGAGAATGCTGAAGGGGATGAGTATGTGGCCGACTTCTCGGAGCAGGGCTCCCCGGCTGCAGCTGCACAGACCGGCCCCGATGTGTATGTCCTGCCCCTCACCGAGGTCTCCTTACCCATGGCCAAGCAGCCGGGTCGCTCCGTGCAACTTCTCAAGTCCACGGACCTGGGCCGGCACAGCCTCCTGTACTTAAAGGAGATTGGCCACGGCTGGTTTGGGAAGGTGTTTTTGGGGGAGGTACACTCGGGCGTCAGTGGCACGCAGGTGGTGGTGAAGGAGCTGAAGGTGAGCGCCAGCGTGCAGGAGCAGATGCAGTTCCTGGAGGAGGCGCAGCCCTACAGGGCCCTGCAGCACAGCAACCTGCTCCAGTGCCTGGCCCAGTGTGCTGAAGTGACCCCCTACCTGCTGGTTATGGAGTTCTGTCCACTGGGGGACCTCAAAGGTTATCTACGGAGCTGCCGGGTGACAGAGTCCATGGCGCCTGACCCACTTACCTTGCAGCGCATGGCGTGCGAGGTGGCGTGTGGCGTCTTGCATCTACATCGACACAACTATGTACACAGTGACCTGGCCCTGAGGAACTGCCTGCTTACGGCTGACCTGACAGTGAAGGTTGGTGATTATGGCCTGTCGCATTGCAAATACAGGGAAGACTATCTCGTGACCGCTGACCAGCTGTGGGTGCCGCTGCGCTGGATCGCGCCAGAGCTGGTGGACGAGGTTCACGGCAACCTGCTGGTGGTGGACCAGACCAAGAGCAGCAACGTGTGGTCCCTGGGTGTGACCATCTGGGAGCTTTTCGAGTTGGGTGCGCAGCCGTACCCCCAGCACTCGGACCGGCAGGTGCTGGCTTACGCCGTCCGGGAGCAGCAGCTTAAGTTGCCCAAGCCCCAGCTGCAGCTGGCTCTATCTGATCGATGGTACGAGGTGATGCAGTTCTGCTGGCTGCAGCCAGAGCAGAGGCCCACAGCTGAAGAGGTTCATCTACTGCTGTCCTACTTGTGTGCTAAGGGCACCACAGAATTGGAGGAGGAGTTTGAGCGGCGCTGGCGCTCCCTGCGGCCCGGGGGCAGCACGGGCCTGGGCTCGGGTTCTGCAGCCCCAGCAGCTGCCACCGCCGCCTCCGCGGAGCTCACCGCTGCCTCGTCTTTCCCGCTGCTGGAGCGGTTCACCAGCGACGGCTTTCACGTGGACAGTGATGACGTGCTGACGGTCACGGAGACAAGCCACGGCCTCAACTTTGAATACAAGTGGGAGGCTGGCTGTGGCGCTGAGGAGTACCCACCCTCGGGGGCTGCATCAAGCCCAGGCTCGGCAGCGCGCCTGCAGGAGTTGTGCGCGCCTGACAGTTCACCGCCGGGTGTGGTGCCAGTCCTCAGTGCCCACAGCCCCTCAGTGGGTAGCGAGTACTTCATCCGCCTGGAGGGGGCAGTGCCTGCTGCTGGCCACGATCCAGACTGTGCCGGCTGCGCTCCCAGCCCCCAGGCTGTGACTGACCAGGACAATAACTCTGAGGAGAGCACTGTTGCGTCCCTTGCCATGGAGCCATTGCTGGGCCATGCACCCCCAACTGAGGGTCTGTGGGGCCCCTGTGACCACCATTCACATAGGAGGCAAGGGTCACCCTGTCCCTCACGCTCACCCTCTCCTGGGACCCCGATGTTGCCAGCTGAAGACATAGACTGGGGTGTGGCTACCTTCTGCCCGCCCTTCTTTGATGACCCACTGGGTGCATCTCCCTCGGGGAGTCCTGGGGCCCAGCCCTCCCCCAGTGATGAGGAGCCAGAGGAGGGGAAGGTGGGGTTGGCCGCTCAGTGTGGACACTGGAGCTCTAACATGTCAGCTAACAACAACAGTGCCAGTCGCGACCCAGAATCCTGGGATCCTGGCTATGTTAGCAGCTTCACAGACAGCTACAGGGACGACTGCTCCAGCTTAGAGCAGACCCCTCGAGCCTCCCCTGAGGTGGGCCATCTCCTGTCCCAGGAGGATCCCAGAGATTTTCTCCCTGGGCTAGTAGCAGTCTCCCCTGGTCAGGAGCCAAGCCGTCCCTTCAACCTGCTCCCCCTGTGTCCTGCCAAAGGCCTGGCACCTGCTGCCTGCCTCATCACATCCCCTTGGACAGAGGGAGCTGTGGGTGGGGCTGAGAACCCCATTGTGGAACCCAAACTTGCCCAGGAGGCTGAGGGATCTGCTGAACCCCAGCTACCCCTTCCTTCCGTCCCCTCCCCATCCTGTGAAGGAGCCTCACTTCCCTCGGAGGAGGCAAGCGCTCCTGACATCCTACCTGCCTCACCCACACCCGCTGCTGGCAGCTGGGTGACCGTCCCTGAGCCAGCCCCCACCCTGGAGAGCAGCGGCAGTTCTCTGGGGCAAGAGGCACCCAGCAGCGAGGACGAAGACACGACCGAGGCAACATCAGGAGTCTTCACCGACCTGTCCAGTGACGGCCCACACACGGAGAAGTCAGGCATAGTACCAGCCTTGCGCAGTCTGCAGAAGCAGGTGGGGACCCCTGACTCCCTGGACTCTCTGGACATACCGTCCTCAGCCAGTGATGGTGGCTGTGAGGTCTTAAGCCCATCAGCTGCTGGTCCACCTGGCGGGCAGCCCCGTGCTGTGGACAGTGGCTATGATACAGAGAACTATGAGTCTCCTGAGTTTGTGCTCAAGGAGGCCCATGAGTCGAGTGAGCCTGAGGCCTTTGGGGAGCCAGCCTCAGAGGGTGAAAGCCCAGGGCCCGATCCTCTGCTCTCTGTCTCCCTTGGTGGCCTCAGCAAGAAGAGCCCCTACCGAGACTCTGCCTACTTTTCAGACCTGGATGCCGAGTCCGAACCCACCTTTGGCCCTGAGAAGCACAGTGGGATCCAGGACTCCCAAAAGGAGCAAGACCTGAGGAGCCCACCTAGCCCAGGGCATCAGTCTGTGCAGGCTTTTCCCAGGTCTGCAGTGTCCAGCGAGGTGCTGTCCCCTCCACAGCAGTCAGAGGAGCCCTTGCCAGAAGTCCCCAGGCCAGAGCCTCTCGGGGCTCAAGGCCCAGTTGGAGTGCAGCCTGTGCCCGGCCCAAGTCATTCCAAATGTTTCCCGCTGACCTCCGTTCCACTGATCTCAGAAGGCAGTGGCACGGAGCCCCAGGGTCCCTCAGGACAGCTGTCAGGGCGAGCCCAGCAGGGGCAGATGGGCAATCCTAGCACACCCAGATCCCCGCTCTGCCTGGCCCTGCCTGGCCACCCCGGGGCTTTGGAGGGCCGGCCGGAGGAGGATGAGGACACCGAGGACAGCGAGGAGTCTGATGAGGAGCTCCGGTGCTACAGCGTCCAGGAGCCCAGTGAGGACAGCGAGGAGGAGCCGCCAGCGGTGCCCGTGGTGGTGGCTGAGAGCCAGAGTGCCCGAAATCTACGCAGCCTGCTGAAGATGCCCAGCCTGCTGTCAGAGGCCTTCTGTGACGACCTGGAGCGCAAGAAGAAGGCTGTGTCCTTCTTCGATGATGTCACGGTCTACCTCTTTGACCAGGAAAGCCCCACCCGAGAGACTGGGGAGCCCTTTCCCAGCACAAAGGAATCACTCCCTACGTTCCTGGAGGGTGGCCCCAGCTCACCCAGTGCCACCGGCCTGCCACTGCGGGCTGGCCACTCTCCTGACAGCTCTGCTCCTGAACCAGGCAGTAGGTTCGAATGGGATGGTGATTTCCCGTTGGTGCCCGGCAAGGCTGCTTTGGTGACTGAGCTGGATCCTGCTGACCCTGTCCTGGCGGCGCCTCCCACGCCAGCTGCGCCCTTCTCACGCTTCACCGTGTCACCCACACCTGCCTCCCGCTTTTCCATCACCCACATATCTGACTCAGATGCCCAGTCCGTGGGAGGCCCAGCAGCAGGTGCTGGGGGCCGATACACAGAGGCTTGAGCGGCCGC

Amino Acid Sequence

Protein sequence of expressed construct, D254/V+D272/A
R1327/A,R1337/A BOLD MDYKDDDDKGSMSSSFFNPSFAFSSHFDPDGAPLSELSWSSSLAVVAVSFSGIFTVVILMLACLCCKKGGIGFKEFENAEGDEYVADFSEQGSPAAAAQTGPDVYVLPLTEVSLPMAKQPGRSVQLLKSTDLGRHSLLYLKEIGHGWFGKVFLGEVHSGVSGTQVVVKELKVSASVQEQMQFLEEAQPYRALQHSNLLQCLAQCAEVTPYLLVMEFCPLGDLKGYLRSCRVTESMAPDPLTLQRMACEVACGVLHLHRHNYVHSVLALRNCLLTADLTVKVGAYGLSHCKYREDYLVTADQLWVPLRWIAPELVDEVHGNLLVVDQTKSSNVWSLGVTIWELFELGAQPYPQHSDRQVLAYAVREQQLKLPKPQLQLALSDRWYEVMQFCWLQPEQRPTAEEVHLLLSYLCAKGTTELEEEFERRWRSLRPGGSTGLGSGSAAPAAATAASAELTAASSFPLLERFTSDGFHVDSDDVLTVTETSHGLNFEYKWEAGCGAEEYPPSGAASSPGSAARLQELCAPDSSPPGVVPVLSAHSPSVGSEYFIRLEGAVPAAGHDPDCAGCAPSPQAVTDQDNNSEESTVASLAMEPLLGHAPPTEGLWGPCDHHSHRRQGSPCPSRSPSPGTPMLPAEDIDWGVATFCPPFFDDPLGASPSGSPGAQPSPSDEEPEEGKVGLAAQCGHWSSNMSANNNSASRDPESWDPGYVSSFTDSYRDDCSSLEQTPRASPEVGHLLSQEDPRDFLPGLVAVSPGQEPSRPFNLLPLCPAKGLAPAACLITSPWTEGAVGGAENPIVEPKLAQEAEGSAEPQLPLPSVPSPSCEGASLPSEEASAPDILPASPTPAAGSWVTVPEPAPTLESSGSSLGQEAPSSEDEDTTEATSGVFTDLSSDGPHTEKSGIVPALRSLQKQVGTPDSLDSLDIPSSASDGGCEVLSPSAAGPPGGQPRAVDSGYDTENYESPEFVLKEAHESSEPEAFGEPASEGESPGPDPLLSVSLGGLSKKSPYRDSAYFSDLDAESEPTFGPEKHSGIQDSQKEQDLRSPPSPGHQSVQAFPRSAVSSEVLSPPQQSEEPLPEVPRPEPLGAQGPVGVQPVPGPSHSKCFPLTSVPLISEGSGTEPQGPSGQLSGRAQQGQMGNPSTPRSPLCLALPGHPGALEGRPEEDEDTEDSEESDEELRCYSVQEPSEDSEEEPPAVPVVVAESQSARNLRSLLKMPSLLSEAFCDDLERKKKAVSFFDDVTVYLFDQESPTRETGEPFPSTKESLPTFLEGGPSSPSATGLPLRAGHSPDSSAPEPGSRFEWDGDFPLVPGKAALVTELDPADPVLAAPPTPAAPFSAFTVSPTPASAFSITHISDSDAQSVGGPAAGAGGRYTEA*

• Vector Type: Mammalian
• Antibiotic: Amp
• DU Number: DU10161
• Unit Source: Protein Phosphorylation and Ubiquitylation Unit
• Location: PPU
• MTA: Dundee