cDNA Clone UBA3; APPBP1

• Synonyms: UBA3, Ubiquitin-Activating Enzyme E1 Homolog
• Plasmid: pOPC UBA3 6His, DAC APPBP1
• Parent Plasmid: pOPC
• Expressed: UBA3 6His DAC APPBP1
• Genbank: NP_003959.3 NP_003896
• Tag: DAC
• Species: Human

Sequence of Insert

DNA sequence of UBA3 insert
TCTAGAAATAATTTTGTTTAACTTTAAGAAGGAGATATACATATGGCGGA
TGGCTGTTGATGGTGGGTGTGGGGACACTGGAGACTGGGAAGGTCGCTGG
AACCATGTAAAGAAGTTCCTCGAGCGATCTGGACCCTTCACACACCCTGA
TTTCGAACCGAGCACTGAATCTCTCCAGTTCTTGTTAGATACATGTAAAG
TTCTAGTCATTGGAGCTGGCGGCTTAGGATGTGAGCTCCTGAAAAATCTG
GCCTTGTCTGGTTTTAGACAGATTCATGTTATAGATATGGACACTATAGA
TGTTTCCAATCTAAATAGGCAGTTTTTATTTAGGCCTAAAGATATTGGAA
GACCTAAGGCTGAAGTTGCTGCAGAATTTCTAAATGACAGAGTTCCTAAT
TGCAATGTAGTTCCACATTTCAACAAGATTCAAGATTTTAACGACACTTT
CTATCGACAATTTCATATTATTGTATGTGGACTGGACTCTATCATCGCCA
GAAGATGGATAAATGGCATGCTGATATCTCTTCTAAATTATGAAGATGGT
GTCTTAGATCCAAGCTCCATTGTCCCTTTGATAGATGGGGGGACAGAAGG
TTTTAAAGGAAATGCCCGGGTGATTCTGCCTGGAATGACTGCTTGTATCG
AATGCACGCTGGAACTTTATCCACCACAGGTTAATTTTCCCATGTGCACC
ATTGCATCTATGCCCAGGCTACCAGAACACTGTATTGAGTATGTAAGGAT
GTTGCAGTGGCCTAAGGAGCAGCCTTTTGGAGAAGGGGTTCCATTAGATG
GAGATGATCCTGAACATATACAATGGATTTTCCAAAAATCCCTAGAGAGA
GCATCACAATATAATATTAGGGGTGTTACGTATAGGCTCACTCAAGGGGT
AGTAAAAAGAATCATTCCTGCAGTAGCTTCCACAAATGCAGTCATTGCAG
CTGTGTGTGCCACTGAGGTTTTTAAAATAGCCACAAGTGCATACATTCCC
TTGAATAATTACTTGGTGTTTAATGATGTAGATGGGCTGTATACATACAC
ATTTGAAGCAGAAAGAAAGGAAAACTGCCCAGCTTGTAGCCAGCTTCCTC
AAAATATTCAGTTTTCTCCATCAGCTAAACTACAGGAGGTTTTGGATTAT
CTAACCAATAGTGCTTCTCTGCAAATGAAATCTCCAGCCATCACAGCCAC
CCTAGAGGGAAAAAATAGAACACTTTACTTACAGTCGGTAACCTCTATTG
AAGAACGAACAAGGCCAAATCTCTCCAAAACATTGAAAGAATTGGGGCTT
GTTGATGGACAAGAACTGGCGGTTGCTGATGTCACCACCCCACAGACTGT
ACTATTCAAACTTCATTTTACTTCTCATCATCACCATCACCATTAAGGAT
CC

DNA sequence of DAC APPBP1
CAATTGATGTCTGCAATCCCGGGTGTACCGCAGATCGATGCGGAGTCCTA
CATCCTGATTGACTATAACTCCGGCAAAGTGCTCGCCGAACAGAACGCAG
ATGTCCGCCGCGATCCTGCCAGCCTGACCAAAATGATGACCAGTTACGTT
ATCGGCCAGGCAATGAAAGCCGGTAAATTTAAAGAAACTGATTTAGTCAC
TATCGGCAACGACGCATGGGCCACCGGTAACCCGGTGTTTAAAGGTTCTT
CGCTGATGTTCCTCAAACCGGGCATGCAGGTTCCGGTTTCTCAGCTGATC
CGCGGTATTAACCTGCAATCGGGTAACGATGCTTGTGTCGCCATGGCCGA
TTTTGCCGCTGGTAGCCAGGACGCTTTTGTTGGCTTGATGAACAGCTACG
TTAACGCACTGGGCCTGAAAAATACCCACTTCCAGACGGTACATGGTCTG
GATGCTGATGGTCAGTACAGCTCCGCGCGAGATATGGCGCTGATCGGCCA
GGCATTGATCCGTGACGTACCGAATGAATACTCGATCTATAAAGAAAAAG
AATTTACGTTTAACGGTATTCGCCAGCTGAACCGTAACGGCCTGTTATGG
GATAACAGCCTGAATGTCGACGGCATCAAAACCGGACACACTGACAAAGC
AGGTTACAACCTTGTTGCTTCTGCGACTGAAGGCCAGATGCGCTTGATTT
CTGCGGTAATGGGCGGACGTACTTTTAAAGGCCGTGAAGCCGAAAGTAAA
AAACTGCTAACCTGGGGCTTCCGTTTCTTTGAAACCGTTAACCCACTGGT
GCCGCGCGGCAGCGGATCTATGGCGCAGCTGGGAAAGCTGCTCAAGGAGC
AGAAGTACGACCGGCAGCTGAGGTTGTGGGGTGATCATGGGCAAGAGGCT
TTAGAATCTGCTCATGTTTGCCTAATAAATGCAACAGCCACAGGAACTGA
AATTCTTAAAAACTTGGTACTACCAGGTATTGGTTCGTTTACAATTATTG
ATGGAAATCAGGTCAGCGGAGAAGATGCTGGAAACAATTTCTTCCTTCAA
AGAAGCAGTATCGGCAAGAACCGAGCTGAAGCTGCCATGGAATTCTTACA
AGAATTAAATAGCGATGTCTCTGGAAGTTTTGTGGAAGAGAGTCCAGAAA
ACCTTCTAGACAATGATCCCTCATTTTTCTGTAGGTTTACTGTTGTAGTT
GCAACTCAGCTTCCTGAAAGCACTTCACTACGCTTAGCAGATGTCCTCTG
GAATTCCCAGATTCCTCTTTTGATCTGTAGGACATATGGACTAGTTGGTT
ATATGAGGATCATTATAAAAGAACATCCAGTAATAGAATCTCATCCAGAT
AATGCATTAGAGGATCTACGACTAGATAAGCCATTTCCTGAACTGAGAGA
ACATTTTCAGTCCTATGATTTGGATCATATGGAAAAAAAGGACCACAGTC
ATACTCCATGGATTGTGATCATAGCTAAATATTTAGCACAGTGGTATAGT
GAAACAAATGGACGAATACCTAAAACGTATAAAGAAAAAGAGGACTTCAG
AGATTTGATTAGACAAGGAATTCTAAAAAATGAAAATGGGGCTCCAGAAG
ATGAAGAGAATTTTGAAGAAGCTATTAAAAATGTGAACACAGCACTAAAT
ACAACTCAGATCCCAAGCAGTATTGAAGATATATTTAATGATGATCGCTG
CATAAATATCACCAAACAGACTCCATCATTTTGGATTTTAGCTCGTGCCT
TAAAGGAATTTGTGGCCAAAGAGGGTCAAGGAAATTTACCTGTTCGAGGC
ACAATTCCTGATATGATTGCAGATTCAGGCAAATATATAAAACTGCAAAA
CGTTTACCGTGAAAAAGCAAAGAAAGATGCTGCCGCTGTGGGTAATCATG
TTGCCAAATTGCTGCAGTCCATTGGCCAGGCACCAGAGTCCATTTCAGAG
AAAGAATTAAAATTACTCTGCAGCAATTCTGCATTTCTTCGAGTGGTAAG
ATGTCGATCCTTAGCTGAAGAATATGGTTTGGATACAATTAACAAGGATG
AAATTATTTCTAGCATGGACAATCCAGATAATGAAATAGTGTTGTACTTA
ATGTTACGGGCTGTTGATAGATTTCATAAACAACAGGGTAGATATCCAGG
AGTATCTAACTATCAAGTTGAAGAAGATATAGGAAAGTTGAAGTCTTGTC
TCACTGGCTTCCTTCAGGAATATGGTTTATCTGTAATGGTGAAAGATGAT
TATGTCCACGAATTTTGCCGATATGGAGCTGCTGAGCCACATACCATTGC
TGCATTCTTGGGGGGAGCTGCTGCTCAAGAGGTCATCAAAATAATCACCA
AACAATTTGTAATTTTTAATAATACTTACATTTACAGTGGCATGTCACAA
ACTTCAGCAACTTTCCAGTTGTAGAAGCTT

Amino Acid Sequence

Protein sequence of UBA3 MAVDGGCGDTGDWEGRWNHVKKFLERSGPFTHPDFEPSTESLQFLLDTCK
VLVIGAGGLGCELLKNLALSGFRQIHVIDMDTIDVSNLNRQFLFRPKDIG
RPKAEVAAEFLNDRVPNCNVVPHFNKIQDFNDTFYRQFHIIVCGLDSIIA
RRWINGMLISLLNYEDGVLDPSSIVPLIDGGTEGFKGNARVILPGMTACI
ECTLELYPPQVNFPMCTIASMPRLPEHCIEYVRMLQWPKEQPFGEGVPLD
GDDPEHIQWIFQKSLERASQYNIRGVTYRLTQGVVKRIIPAVASTNAVIA
AVCATEVFKIATSAYIPLNNYLVFNDVDGLYTYTFEAERKENCPACSQLP
QNIQFSPSAKLQEVLDYLTNSASLQMKSPAITATLEGKNRTLYLQSVTSI
EERTRPNLSKTLKELGLVDGQELAVADVTTPQTVLFKLHFTSHHHHHH*

Protein sequence of DAC APPBP1
MSAIPGVPQIDAESYILIDYNSGKVLAEQNADVRRDPASLTKMMTSYVIG
QAMKAGKFKETDLVTIGNDAWATGNPVFKGSSLMFLKPGMQVPVSQLIRG
INLQSGNDACVAMADFAAGSQDAFVGLMNSYVNALGLKNTHFQTVHGLDA
DGQYSSARDMALIGQALIRDVPNEYSIYKEKEFTFNGIRQLNRNGLLWDN
SLNVDGIKTGHTDKAGYNLVASATEGQMRLISAVMGGRTFKGREAESKKL
LTWGFRFFETVNPLVPRGSGSMAQLGKLLKEQKYDRQLRLWGDHGQEALE
SAHVCLINATATGTEILKNLVLPGIGSFTIIDGNQVSGEDAGNNFFLQRS
SIGKNRAEAAMEFLQELNSDVSGSFVEESPENLLDNDPSFFCRFTVVVAT
QLPESTSLRLADVLWNSQIPLLICRTYGLVGYMRIIIKEHPVIESHPDNA
LEDLRLDKPFPELREHFQSYDLDHMEKKDHSHTPWIVIIAKYLAQWYSET
NGRIPKTYKEKEDFRDLIRQGILKNENGAPEDEENFEEAIKNVNTALNTT
QIPSSIEDIFNDDRCINITKQTPSFWILARALKEFVAKEGQGNLPVRGTI
PDMIADSGKYIKLQNVYREKAKKDAAAVGNHVAKLLQSIGQAPESISEKE
LKLLCSNSAFLRVVRCRSLAEEYGLDTINKDEIISSMDNPDNEIVLYLML
RAVDRFHKQQGRYPGVSNYQVEEDIGKLKSCLTGFLQEYGLSVMVKDDYV
HEFCRYGAAEPHTIAAFLGGAAAQEVIKIITKQFVIFNNTYIYSGMSQTS
ATFQL*

• Vector Type: Bacterial
• Antibiotic: Amp
• DU Number: DU20996
• Unit Source: Protein Phosphorylation and Ubiquitylation Unit
• Location: PPU
• MTA: Dundee
• Notes: Bacterial expression; Parental Plasmid DU 32429 (pOPC UBA3 6HIS); ; Calc Mol Weight of UBA3 : 50163 Daltons; Calc Mol Weight of DAC APPBP1 : 89619 Daltons