cDNA Clone USP19

• Plasmid: pGEX6P-3-USP19 1-1290
• Parent Plasmid: pGEX6P3
• Expressed: GST-USP19 1-1290
• Genbank: NM_006677.1
• Tag: GST
• Species: Human

Sequence of Insert

GTCGACATGTCTGGCGGGGCCAGTGCCACAGGCCCAAGGAGAGGGCCCCC
AGGACTGGAGGACACCACTAGTAAGAAGAAGCAGAAGGATCGAGCAAACC
AGGAGAGCAAGGATGGAGATCCTAGGAAAGAGACAGGGTCTCGATATGTT
GCCCAGGCTGGTCTTGAACCTCTGGCCTCAGGTGATCCTTCTGCCTCAGC
CTCCCATGCAGCTGGGATCACAGGCTCACGCCACCGTACCCGGCTGTTCT
TTCCTTCATCGTCAGGGTCAGCATCCACTCCTCAAGAGGAGCAGACCAAA
GAGGGAGCTTGTGAAGACCCTCATGATCTCTTGGCTACTCCCACTCCAGA
GTTGTTGCTCGATTGGAGGCAGAGTGCAGAAGAGGTGATTGTCAAGCTTC
GTGTGGGAGTAGGTCCCCTGCAGCTGGAGGATGTAGATGCTGCTTTCACA
GATACAGACTGTGTGGTGCGGTTTGCAGGTGGTCAGCAGTGGGGTGGTGT
CTTCTATGCTGAGATAAAAAGCTCTTGTGCTAAAGTGCAAACCCGCAAGG
GCAGTCTCCTGCACCTGACACTGCCCAAAAAGGTGCCTATGCTCACGTGG
CCCTCCCTCCTGGTTGAGGCTGATGAACAGCTTTGCATACCACCGCTGAA
CTCCCAAACCTGCCTCCTGGGCTCAGAGGAGAATTTAGCCCCTTTGGCAG
GAGAGAAAGCAGTGCCTCCCGGGAATGACCCAGTCTCTCCAGCCATGGTC
CGGAGCAGAAACCCTGGGAAAGATGACTGTGCCAAGGAGGAGATGGCAGT
GGCAGCAGATGCTGCAACCTTGGTGGATGAGCCCGAGTCGATGGTGAACC
TGGCGTTTGTCAAGAATGACTCGTATGAGAAGGGCCCGGATTCAGTGGTG
GTGCACGTGTACGTGAAGGAGATCTGCAGGGACACCTCAAGAGTACTTTT
CCGTGAGCAGGACTTCACGCTCATCTTCCAGACCAGGGATGGAAACTTCC
TGAGGCTGCACCCGGGCTGTGGGCCCCACACCACCTTCCGTTGGCAGGTG
AAGCTCAGGAATCTGATTGAGCCAGAGCAGTGCACCTTCTGTTTCACGGC
TTCTCGCATCGACATCTGCCTTCGTAAGAGGCAGAGTCAGCGCTGGGGGG
GCCTGGAGGCCCCGGCTGCACGAGTGGGTGGTGCAAAGGTTGCCGTGCCG
ACAGGTCCAACCCCTCTGGATTCAACCCCACCAGGAGGTGCTCCCCACCC
CCTGACAGGCCAGGAGGAGGCCCGGGCTGTGGAGAAGGATAAATCCAAGG
CACGATCTGAGGACACAGGGCTAGACAGTGTGGCAACCCGCACACCCATG
GAGCATGTAACCCCAAAGCCAGAGACACACCTGGCCTCGCCCAAGCCTAC
ATGCATGGTGCCTCCCATGCCCCACAGCCCAGTTAGTGGAGACAGCGTGG
AGGAGGAGGAAGAGGAAGAGAAGAAGGTGTGTCTGCCAGGCTTCACTGGC
CTTGTCAATTTAGGCAACACCTGCTTCATGAACAGCGTCATTCAGTCTCT
GTCCAACACTCGGGAACTCCGGGACTTCTTCCATGACCGCTCCTTTGAGG
CTGAGATCAACTACAACAACCCACTAGGGACTGGTGGGCGTCTGGCCATT
GGCTTTGCCGTGCTGCTTCGGGCGCTGTGGAAGGGCACCCACCATGCCTT
CCAGCCTTCCAAGTTGAAGGCCATTGTGGCGAGTAAGGCCAGCCAGTTCA
CAGGCTATGCACAGCATGATGCCCAGGAGTTCATGGCTTTCCTGCTGGAT
GGGCTGCACGAGGACCTGAATCGCATTCAGAACAAGCCCTACACAGAGAC
CGTGGATTCAGATGGGCGGCCCGATGAGGTGGTAGCTGAGGAAGCATGGC
AGCGGCACAAGATGAGGAATGACTCTTTCATCGTGGACCTATTTCAGGGG
CAGTACAAGTCGAAGCTGGTGTGCCCTGTGTGTGCCAAGGTCTCCATCAC
TTTTGACCCGTTTCTTTATCTGCCGGTGCCCTTGCCACAAAAGCAAAAGG
TTCTCCCTGTCTTTTATTTTGCCCGAGAGCCCCACAGCAAGCCCATCAAG
TTCCTGGTGAGCGTCAGCAAGGAGAACTCCACTGCGAGCGAAGTATTGGA
CTCCCTCTCTCAGAGTGTTCATGTGAAGCCTGAGAACCTGCGTTTGGCGG
AGGTAATTAAGAATCGTTTTCATCGTGTGTTCCTACCCTCCCACTCACTG
GACACTGTGTCCCCATCTGATACGCTCCTCTGCTTTGAGCTGCTATCCTC
AGAGTTGGCTAAGGAGCGGGTAGTGGTGCTAGAGGTGCAACAGCGCCCCC
AGGTGCCCAGCGTCCCCATCTCCAAGTGTGCAGCCTGCCAGCGGAAGCAA
CAGTCGGAGGATGAAAAGCTGAAGCGCTGTACCCGGTGCTACCGTGTGGG
CTACTGCAACCAGCTCTGCCAGAAAACCCACTGGCCTGACCACAAGGGCC
TCTGCCGACCTGAGAACATTGGCTACCCCTTCCTGGTCAGTGTACCTGCC
TCACGCCTCACTTATGCCCGCCTCGCTCAGTTGCTAGAGGGCTATGCCCG
GTACTCTGTGAGTGTATTCCAGCCACCCTTTCAGCCAGGCCGCATGGCCT
TGGAGTCTCAGAGCCCTGGCTGCACCACACTGCTCTCCACAGGTTCCCTG
GAGGCTGGGGACAGCGAGAGAGACCCCATTCAGCCACCTGAGCTCCAGCT
GGTGACCCCTATGGCTGAGGGGGACACAGGGCTTCCCCGGGTGTGGGCAG
CCCCTGACCGGGGTCCTGTGCCCAGCACCAGTGGAATTTCTTCTGAGATG
CTGGCCAGTGGGCCCATTGAGGTTGGCTCCTTGCCAGCTGGCGAGAGGGT
GTCCCGACCCGAAGCTGCTGTGCCTGGGTACCAGCATCCAAGTGAAGCTA
TGAATGCCCACACACCCCAGTTCTTCATCTATAAAATTGATTCATCCAAC
CGAGAGCAGCGGCTAGAGGACAAAGGAGACACCCCACTGGAGCTGGGTGA
CGACTGTAGCCTGGCTCTCGTCTGGCGGAACAATGAGCGCTTGCAGGAGT
TTGTGTTGGTAGCCTCCAAGGAGCTGGAATGTGCTGAGGATCCAGGCTCT
GCCGGTGAGGCTGCCCGGGCCGGCCACTTCACCCTGGACCAGTGCCTCAA
CCTCTTCACACGGCCTGAGGTGCTGGCACCCGAGGAGGCCTGGTACTGCC
CACAGTGCAAACAGCACCGTGAGGCCTCCAAGCAGCTGTTGCTATGGCGC
CTGCCAAATGTTCTCATCGTGCAGCTCAAGCGCTTCTCCTTTCGTAGTTT
TATCTGGCGTGACAAGATCAATGACTTGGTGGAGTTCCCTGTTAGGAACC
TGGACCTGAGCAAGTTCTGCATTGGTCAGAAAGAGGAGCAGCTGCCCAGC
TACGATCTATATGCTGTCATCAACCACTATGGAGGCATGATTGGTGGCCA
CTACACTGCCTGTGCACGCCTGCCCAATGATCGTAGCAGTCAGCGCAGTG
ACGTGGGCTGGCGCTTGTTTGATGACAGCACAGTGACAACGGTAGACGAG
AGCCAGGTTGTGACGCGTTATGCCTATGTACTCTTCTACCGCCGGCGGAA
CTCTCCTGTGGAGAGGCCCCCCAGGGCAGGTCACTCTGAGCACCACCCAG
ACCTAGGCCCTGCAGCTGAGGCTGCTGCCAGCCAGGCTTCCCGGATTTGG
CAGGAGCTGGAGGCTGAGGAGGAGCCGGTGCCTGAGGGGTCTGGGCCCCT
GGGTCCCTGGGGGCCCCAAGACTGGGTGGGCCCCCTACCACGTGGCCCTA
CCACACCAGATGAGGGCTGCCTCCGGTGAGCGGCCGC

Amino Acid Sequence

MSPILGYWKIKGLVQPTRLLLEYLEEKYEEHLYERDEGDKWRNKKFELGL
EFPNLPYYIDGDVKLTQSMAIIRYIADKHNMLGGCPKERAEISMLEGAVL
DIRYGVSRIAYSKDFETLKVDFLSKLPEMLKMFEDRLCHKTYLNGDHVTH
PDFMLYDALDVVLYMDPMCLDAFPKLVCFKKRIEAIPQIDKYLKSSKYIA
WPLQGWQATFGGGDHPPKSDLEVLFQGPLGSPNSRVDMSGGASATGPRRG
PPGLEDTTSKKKQKDRANQESKDGDPRKETGSRYVAQAGLEPLASGDPSA
SASHAAGITGSRHRTRLFFPSSSGSASTPQEEQTKEGACEDPHDLLATPT
PELLLDWRQSAEEVIVKLRVGVGPLQLEDVDAAFTDTDCVVRFAGGQQWG
GVFYAEIKSSCAKVQTRKGSLLHLTLPKKVPMLTWPSLLVEADEQLCIPP
LNSQTCLLGSEENLAPLAGEKAVPPGNDPVSPAMVRSRNPGKDDCAKEEM
AVAADAATLVDEPESMVNLAFVKNDSYEKGPDSVVVHVYVKEICRDTSRV
LFREQDFTLIFQTRDGNFLRLHPGCGPHTTFRWQVKLRNLIEPEQCTFCF
TASRIDICLRKRQSQRWGGLEAPAARVGGAKVAVPTGPTPLDSTPPGGAP
HPLTGQEEARAVEKDKSKARSEDTGLDSVATRTPMEHVTPKPETHLASPK
PTCMVPPMPHSPVSGDSVEEEEEEEKKVCLPGFTGLVNLGNTCFMNSVIQ
SLSNTRELRDFFHDRSFEAEINYNNPLGTGGRLAIGFAVLLRALWKGTHH
AFQPSKLKAIVASKASQFTGYAQHDAQEFMAFLLDGLHEDLNRIQNKPYT
ETVDSDGRPDEVVAEEAWQRHKMRNDSFIVDLFQGQYKSKLVCPVCAKVS
ITFDPFLYLPVPLPQKQKVLPVFYFAREPHSKPIKFLVSVSKENSTASEV
LDSLSQSVHVKPENLRLAEVIKNRFHRVFLPSHSLDTVSPSDTLLCFELL
SSELAKERVVVLEVQQRPQVPSVPISKCAACQRKQQSEDEKLKRCTRCYR
VGYCNQLCQKTHWPDHKGLCRPENIGYPFLVSVPASRLTYARLAQLLEGY
ARYSVSVFQPPFQPGRMALESQSPGCTTLLSTGSLEAGDSERDPIQPPEL
QLVTPMAEGDTGLPRVWAAPDRGPVPSTSGISSEMLASGPIEVGSLPAGE
RVSRPEAAVPGYQHPSEAMNAHTPQFFIYKIDSSNREQRLEDKGDTPLEL
GDDCSLALVWRNNERLQEFVLVASKELECAEDPGSAGEAARAGHFTLDQC
LNLFTRPEVLAPEEAWYCPQCKQHREASKQLLLWRLPNVLIVQLKRFSFR
SFIWRDKINDLVEFPVRNLDLSKFCIGQKEEQLPSYDLYAVINHYGGMIG
GHYTACARLPNDRSSQRSDVGWRLFDDSTVTTVDESQVVTRYAYVLFYRR
RNSPVERPPRAGHSEHHPDLGPAAEAAASQASRIWQELEAEEEPVPEGSG
PLGPWGPQDWVGPLPRGPTTPDEGCLR*

• Vector Type: Bacterial
• Antibiotic: Amp
• DU Number: DU20876
• Unit Source: Protein Phosphorylation and Ubiquitylation Unit
• Location: PPU
• MTA: Dundee
• Notes: Bacterial expression construct for GST- USP19 aa1-1290 W103A C163A; ; ; Estimated MW of expressed protein : 169980 Daltons